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mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between Sal1 and NotI sites of pSPORT1. The Mlu1-Sal1 fragement come from the adaptator used for the cloning. The 3' end is at the NotI site. cDNA corresponding to abundant species were eliminated from this library.
Nucleotide
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