NCI_CGAP_FT2 is a subtracted cDNA library constructed from a pool of 81 RNA samples from Alveolar Macrophages challenged with different treatments. The mRNA samples were a mixture of these conditions (times refer to incubations following isolation by bronchoalveolar lavage) (some normal donor macrophages were cultured in some of the conditions, other donor macrophages in different conditions). The mRNA samples were pooled for library construction. Control 0 hours; control 3 hours; control 24 hours; LPS 100 ng/ml, 3 hours; LPS 100 ng/ml, 24 hours; PMA 10 ng/ml, 3 hours; PMA 10 ng/ml, 24 hours; Klebsiella moi 10, 3 hours; Klebsiella moi 10, 24 hours; Staph aureus moi 10, 3 hours; Staph aureus moi 10, 24 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 3 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 24 hours; wt adenovirus moi 500, 3 hours; wt adenovirus moi 500, 24 hours; Ad vector + LPS 3 hours; Ad vector + LPS 24 hours; wt adenovirus + LPS 3 hours; wt adenovirus + LPS 24 hours. The library was subtracted according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The tissue was provided by Dr. Gary W. Hunninghake of the University of Iowa.