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zebrafish fin day3 regeneration

Identifiers
BioSample: SAMN00155616; EST: LIBEST_001028
Organism
Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio
Attributes
sexmixed male and female
tissue3 day fin regenerates
lab hostE. coli XLOLR
vectorpBK-CMV
v_typephagemid
re_1EcoRI
re_2XhoI
Description

1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag- 3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBK-CMV phagemid.

Submission
Washington University School of Medicine, Stephen L. Johnson; 1997-11-05
Accession:
SAMN00155616
ID:
155616

Nucleotide

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