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Normalized endosperm cDNA Library of Maize hybrid YUYU22

Identifiers
BioSample: SAMN00154069; EST: LIBEST_015370
Organism
Zea mays
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
Attributes
cultivarYUYU22
sexhermaphrodite
tissuesingle tissues
development stagemultiple stages
lab hostE.coliDH10B
tissuesingle organs
vectorpSPORT1
v_typePlasmid
re_1Not1
re_2Sal1
Description

mRNA was isolated from endosperm 4 days after pollinating and 8 DAP and 14 DAP and 18 DAP and 26 DAP and 40 DAP. Individual libraries were made according to the SuperScript cDNA Synthesis and Plasmid Cloning Kit (Invitrogen). Normalized cDNA library was made by saturation hybridization with genomic DNA.

Submission
Huazhong Agricultural University of China, PI Yuan-jiang; ZHANG Fang-dong ; ZHANG Zu-xin;ZHENG Yong-lian;xu shang zhong.; 2004-02-10
Accession:
SAMN00154069
ID:
154069

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