Soares_Dieckgraefe_colon_NHUC

Warning: The NCBI web site requires JavaScript to function. more...

Result Filters

Organism: Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo

Attributes

tissue	colonic mucosa from 5 ulcerative colitis patients
lab host	DH10B (phage-resistant)
tissue	colon
vector	pT7T3D-PacI
v_type	phagemid (ampicillin resistant)
re_1	Not I
re_2	Eco RI

Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTAGCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from mild cryptitis to severe ulceration, fibrosis, and degeneration. Library constructed by Bento Soares and M. Fatima Bonaldo.