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Plasmid DNA from the normalized library NCI_CGAP_Kid5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1323912-1325831, 1471368-1472903 and 1492104-1493255). Subtraction by Bento Soares and M. Fatima Bonaldo.
Nucleotide
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