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vector: http://www.rzpd.de/info/vectors/pQE80LSN_cloned_pic.shtml ; 1st strand cDNA was prepared from mRNA obtained from human T-Lymphocytes with a NotI - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to SalI adaptors, digested with NotI and cloned into the NotI and SalI sites of the pQE80LSN_cloned vector
Nucleotide
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