In study related to this BioProject we examined genomic, phylogenetic, and physiologic features of Eubacterium limosum and 11 closely related strains. The genomes of the following eubacterial strains FD (DSM 3662T), Marburg (DSM 3468), 2A (DSM 2593), 11A (DSM 2594), G14 (DSM 107592), and 32 (DSM 20517) were sequenced and subsequently compared with previously available genomes of the E. limosum type strain, as well as the strains B2, KIST612, YI (DSM 105863T), and SA11. This comparison revealed a close relationship of all eleven eubacterial strains, forming three distinct clades: E. limosum, E. callanderi, and E. maltosivorans.