BioSample

Approximately 50 adult tarsi mouthparts were dissected from female adults and stored immediately in RNAlater. Total RNA was purified using RNeasy (Qiagen, USA) or RNAqueous (Ambion, USA) kits according to the manufacturer's protocol. The purified RNA was then treated with DNaseI (Ambion, USA) at 37 degrees C for 30 min along with the manufacturer's protocol, quantified and qualified by NanoDrop ND-2000 (Thermo Scientific, USA) as well as 2100 Bioanalyzer (Agilent, USA). The cDNA templates were prepared from purified RNA samples using SuperScript III Reverse Transcriptase (Invitrogen, USA), according to the manufacturer's manual. Briefly, the first-strand cDNA was synthesized with Oligo(dT)20 primer at 65 degrees C for 5 min, and then at 50 degrees C for 60 min. The reaction was stopped at 70 degrees C for 15 min. The templates were stored at -20 degrees C until use. Sequencing was performed at BGI-Tech with Illumina HiSeq 2000.