Tissues were collected from a single immature (~ 1-year-old) male double-haploid fish and frozen in liquid nitrogen and stored at -80C until total RNA isolation. Total RNA was isolated using TRIzol™ (Invitrogen, Carlsbad, CA) from fourteen tissues; white muscle, red muscle, testis, spleen, kidney, head kidney, pituitary, stomach, brain, heart, intestine, gill, skin, liver. Equal masses of total RNAs from various tissues were pooled and used to construct the 454 library.