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SIRO red - SIRO green/red suppression subtractive cDNA library of Hot pepper. The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.
Nucleotide
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