Figure 4. Representative FACS plots illustrating isolation of TALEN expressing hESCs.

Figure 4Representative FACS plots illustrating isolation of TALEN expressing hESCs.

(A) Mock electroporated hESCs (cells electroporated without TALEN plasmid DNA). (B) TALEN-electroporated hESCs. Percentage of P2-gated cells expressing GFP and/or RFP based on gates shown: GFP only 12%, GFP + RFP 0.7%, RFP only < 0.1%. For the electroporated sample, cells from the GFP, RFP, and GFP + RFP gates are sorted into a single 1.5 mL collection tube.

From: Genome editing in human pluripotent stem cells

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Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2013 Derek T. Peters, Chad A. Cowan and Kiran Musunuru.

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