Figure 5. Representative images showing the expansion and screening of hESC lines during the genome editing process.

Figure 5Representative images showing the expansion and screening of hESC lines during the genome editing process.

(A) A hESC colony 12 days after clonal plating following FACS. (B) hESCs in a 96-well 1 day after manual picking. (C) hESCs in a 96-well 4 days after picking when they are ready to be passaged. (D) PCR screening the targeted genomic locus using genomic DNA from individual hESC clones. Clones that likely harbor TALEN-generated mutations are indicated (arrows).

From: Genome editing in human pluripotent stem cells

Cover of StemBook
StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2013 Derek T. Peters, Chad A. Cowan and Kiran Musunuru.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.