Overview of the Trim-NHL protein family. The mammalian NHLrc1-3 proteins are not considered due to space constraints and because of the lack of Trim motifs in these proteins (with the exception of the RING in NHLrc1). Protein motifs were identified using the SMART (http://smart.embl-heidelberg.de), InterProScan (http://www.ebi.ac.uk/Tools/InterProScan/) and ProSitescan (http://expasy.org/tools/scanprosite/) tools. A phylogenetic tree was generated after ClustalW alignment of the C-terminal sequences. The relationships may vary from published phylogenies depending on the sequences chosen for alignment and precise methodologies used. For display, the x-axis was compressed six-fold. C. elegans proteins are in light gray (green), D. melanogaster in medium gray (red), M. musculus in black (blue) type. A color version of this figure is available at www.landesbioscience.com/curie.