Sanger sequencing

A method of DNA sequencing that uses DNA polymerase to copy single-stranded DNA templates by adding nucleotides to form a complementary strand. Its use is limited to sequence analysis of a single region of DNA (maximum ~1000 bp) - in contrast to massively parallel sequencing, in which millions of fragments of DNA can be sequenced simultaneously.

Related terms: deletion/duplication analysis; molecular genetic testing; PCR; targeted analysis for pathogenic variants