This manual documents the BLAST (Basic Local Alignment Search Tool) command line applications developed at the National Center for Biotechnology Information (NCBI).

Contents

• Introduction to BLAST
  • BLAST Help Manual Overview
    Tom Madden.
    Created: January 28, 2011.
• User Manuals
  • Standalone BLAST Setup for Windows PC
    Tao Tao.
    Created: May 31, 2010; Last Update: August 31, 2020.
  • Standalone BLAST Setup for Unix
    Tao Tao.
    Created: May 31, 2010; Last Update: August 31, 2020.
  • BLAST+ Release Notes
    Christiam Camacho and Tom Madden.
    Created: March 12, 2013; Last Update: October 24, 2023.
  • BLAST FTP Site
    Tao Tao, Tom Madden, and Camacho Christiam.
    Created: May 29, 2011; Last Update: August 30, 2020.
• BLAST Glossary
  Jan Fassler and Peter Cooper.
  Created: July 14, 2011.