2AHM


Conserved Protein Domain Family
CoV_NSP8
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pfam08717: CoV_NSP8 
Coronavirus replicase NSP8
Viral NSP8 (non structural protein 8) forms a hexadecameric supercomplex with NSP7 that adopts a hollow cylinder-like structure. The dimensions of the central channel and positive electrostatic properties of the cylinder imply that it confers processivity on RNA-dependent RNA polymerase. NSP7 and NSP8 heterodimers play a role in the stabilization of NSP12 regions involved in RNA binding and are essential for a highly active NSP12 polymerase complex. It has been demonstrated that NSP8 acts as an oligo(U)-templated polyadenylyltransferase but also has robust (mono/oligo) adenylate transferase activities. NSP8 has N- and C-terminal D/ExD/E conserved motifs, being the N-terminal motif critical for RNA polymerase activity as these residues are part of the Mg2-binding active site.
Links
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Statistics
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PSSM-Id: 400866
Aligned: 6 rows
Threshold Bit Score: 332.58
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
2AHM_E    6 AIASEFSSLPSYAAYATAQEAYEQA---VANGDS-EVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARS 81  
P0C6V0 4011 ALQSEFVNMASFVEYELAKKNLDEA---KASGSAnQQQIKQLEKACNIAKSAYERDRAVARKLERMADLALTNMYKEARI 4087 Murine hepatitis vi...
P0C6U8 3920 AIASEFSSLPSYAAYATAQEAYEQA---VANGDS-EVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARS 3995 Severe acute respir...
Q98VG9 3583 SVASAYAALPSWIAYEKARADLEEA---KKNDVS-PQLLKQLTKACNIAKSEFEREASVQKKLDKMAEQAAASMYKEARA 3658 Feline infectious p...
P0C6U2 3630 SVASSFVGMPSFVAYETARQEYENA---VANGSS-PQIIKQLKKAMNVAKAEFDRESSVQKKINRMAEQAAAAMYKEARA 3705 Human coronavirus 229E
P0C6V3 3463 SVTQEFSHIPSYAEYERAKNLYEKVlvdSKNGGVtQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARV 3542 Avian infectious br...

2AHM_E   82 EDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVVPDYGTYKNTCDGNTFTYASALWEI 161 
P0C6V0 4088 NDKKSKVVSALQTMLFSMVRKLDNQALNSILDNAVKGCVPLNAIPSLTSNTLTIIVPDKQVFDQVVDNVYVTYAGNVWHI 4167 Murine hepatitis vi...
P0C6U8 3996 EDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVVPDYGTYKNTCDGNTFTYASALWEI 4075 Severe acute respir...
Q98VG9 3659 VDRKSKIVSAMHSLLFGMLKKLDMSSVNTIIEQARNGVLPLSIIPAASATRLIVVTPNLEVLSKVRQENNVHYAGAIWSI 3738 Feline infectious p...
P0C6U2 3706 VNRKSKVVSAMHSLLFGMLRRLDMSSVDTILNMARNGVVPLSVIPATSAARLVVVVPDHDSFVKMMVDGFVHYAGVVWTL 3785 Human coronavirus 229E
P0C6V3 3543 TDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVIPDPETWVKCVEGVHVTYSTVVWNI 3622 Avian infectious br...

2AHM_E  162 QQVVDADSKIVQLSEINMD-----NspnlAWPLIVTALRANSA---VKL 202 
P0C6V0 4168 QFIQDADGAVKQLNEIDVN-----S----TWPLVIAANRHNEVs-tVVL 4206 Murine hepatitis virus strain A59
P0C6U8 4076 QQVVDADSKIVQLSEINMD-----NspnlAWPLIVTALRANSA---VKL 4116 Severe acute respiratory syndrome-related coronavirus
Q98VG9 3739 VEVKDANGAQVHLKEVTAA-----NelniTWPLSITCERT------TKL 3776 Feline infectious peritonitis virus (strain 79-1146)
P0C6U2 3786 QEVKDNDGKNVHLKDVTKE-----NqeilVWPLILTCERV------VKL 3823 Human coronavirus 229E
P0C6V3 3623 DTVIDADGTELHPTSTGSGltyciSganiAWPLKVNLTRNGHNkvdVVL 3671 Avian infectious bronchitis virus (strain Beaudette)
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