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Betacoronavirus single-stranded poly(A) binding domain This domain identifies non-structural protein NSP3, the product of ORF1a in group 2 coronavirus. It is found in human SARS coronavirus polyprotein 1a and 1ab, and in related coronavirus polyproteins. NSP3 binds to viral RNA, nucleocapsid protein, as well as other viral proteins, and participates in polyprotein processing. The domain exhibits a macrodomain fold containing the nsp3 residues 528 to 648, with a flexibly extended N-terminal tail from residues 513 to 527 and a C-terminal flexible tail of residues 649 to 651. SUD-M(527-651) binds single-stranded poly(A); the contact area with this RNA on the protein surface, and the electrophoretic mobility shift assays confirm that SUD-M has higher affinity for purine bases than for pyrimidine bases.
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Jiyao W et al.(2023). "The conserved domain database in 2023.",