Conserved Protein Domain Family
WASH-4_N

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pfam14745: WASH-4_N 
WASH complex subunit 4, N-terminal
This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.
Statistics
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PSSM-Id: 434178
Aligned: 17 rows
Threshold Bit Score: 506.473
Created: 25-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_015795193   23 GSFLNEFDSKLEELHKV----------------NLQYTYSSIVYGSYfnpgl-detvhERTEFVDILRTcGNMELNRVLL 85  
XP_001602448   28 GQFFENLAEKCWQESSSl--------------eYVIEGPIRLIYKVY-----------EDISILSLAET-ENKTFSKLLA 81  
XP_624277      28 GQFFENLAKQTWKKDSTl--------------eYFMEGPLRLVYKPV-----------EDISLISLIEM-ENKFLSKLLA 81  
XP_019883503   28 GEFFENLMEKSWRGDSMl--------------eYIIEGPLRLVYKAK-----------ESISILSLVEM-ENKALSKLLA 81  
XP_002431643   28 GKFIEKHTNKLRSIEESfte----------snkNFGYDPINVKLNGW-----------EQIELINLINT-DNKILNKVVL 85  
ESO00575        8 eSFVNDFTANLHNLQIStdvfs-------htvwDIVLNPIALTFHPN-----------ELASIDELIET-DNIIFDKILR 68  
EFA80426       43 NQFAVDYAQQIWQIEEAldetl-------sessDLNVDPVSLYIRPY-----------EQQDIIELIKT-EHKIFNKVML 103 
XP_001020690   46 sqqfnNYIQKTKDLDQKy--------------------qIPLGDTPFnilhlnvnqqvQKQGLFDVNQT-DNPFYDKVSA 104 
XP_002067587   11 GAFIEEHDAHLDKLKTAispggndkltaatvslSLVAPPVEMNYGN------------ANFMISEMNAEaAELLANKPLT 78  
XP_002055003   18 GTFMEQHDEKMQLLQKQmlnnnsldeiggcqllTSVAPAIEINCIES-----------HLISASNLKFSeAELLANKPLT 86  
XP_015795193   86 VLSSLCHEMNNLSQIGLKdFIHIIALYGEdkkkadwkndttkakeSNDEAGdeLFSMSKLLPTLFKLLCFIKRCREVVHN 165 
XP_001602448   82 SVGGTCREIRLLKAEANK-FYEKLFSHGE----------------RCADQE--GYKMENLLSDLQDLSIYTNRIYTVVHL 142 
XP_624277      82 AVAGTCREIRLLVMEAKT-FYKKLFTHGE----------------KGTENN--QNNITSLLSDLQDLFVFVNRIWTVTHL 142 
XP_019883503   82 AVAGTCREIRLLKVEAAG-FYTKLFTHGE----------------RCADDDfpITNVTKLLSDLQDLSIFANRMSTVVHL 144 
XP_002431643   86 VLASLCMEVKERREEGYSkFCNALLKYGEe--------------cSNDDEKilHITISNYLPLFQDLYCFVNRCHQIVIE 151 
ESO00575       69 VFASLCIEIQTLEREAVEnFYLQLIFYGEgd------------ehAEKQDGdiHLQIGRMLPILVGLYKYVGRCTDVLRN 136 
EFA80426      104 VFASLTNQIRILKETAELkFYQPLIVFGEit--------------geASEGdvQVEVGRLLPFMIDLSAFVNRCYAITRN 169 
XP_001020690  105 TFTYLDTEIGKITEVFESkILNVISMYGEralddf-----qdeeeFPLLDGqaEFEISKLSPIFKQSLDLIKKIMGILHN 179 
XP_002067587   79 TLANLCNQCHDLTTALKQ--YQLAFSFGDfqi-------------deGGIEqtLHRMSSAIDFYCQVYFVLKRSIVILQN 143 
XP_002055003   87 TLANLCNQCNDLSKSADQ--LQLKFLNAGtqienlq---islgisPNMGLEhiLCKMSSAIDFFCQIYFLLKRIIVVLQN 161 
XP_015795193  166 LAIQMQELFTrnngkkvnsrsgIEVNDVRFDALFQHLGQLAVTIVNIEEILCSQTSLRKDFINFKRTFEIVIAKYDDFQL 245 
XP_001602448  143 TVRQLSSLAN------------SSHREIYLPLLIEHLMDLFVILVTLDELISNQSALAEQWKVYRVKVRSVLHNAAQLNI 210 
XP_624277     143 TTEQLSNLAS-------------DSNDVYLPVLIEHFIDLFIIVLTLDEIVESQPSLQEQWKKYRMNVHSIMHNPSQFGI 209 
XP_019883503  145 TTQQLASLSG-------------AVHEIYLPTLIEGFIDLFVIFMILDEIIDAQPTIVDQWKKYRMHVRSVMHNPSQFGI 211 
XP_002431643  152 IIKQLAAIYTtsdk-----nltINSMNVHFEDMMDHLGEILTILVTLDRLIESIDNISEGWSLYKRLIRTV-----QLNV 221 
ESO00575      137 CLLQLCIFYTnesa----sirsMDISGSNLTVVYEHIGQLMHHLAVLDHVIDNQTLLKEHWKMYKRMIKSVYSSPESVKV 212 
EFA80426      170 VVSQLASCYQnqtta---ftkfFK--NVHLQTVFYSLADLFTVLITLDQVVTQNQALSSSWGRFQRMVKSVRAEPGKYGV 244 
XP_001020690  180 LINQLNIIYYkkndl-----hknifKNVELNSVFQTIGKGLSVVYVLDCAINQNKKIRDHWNVYKRMIKIIQTEPSKFGS 254 
XP_002067587  144 LWNQISASVCl----------pVDIGEVHLFTTFDSICELLEQIVIFNEI-SMQSNMSEKWTLYKKWLKNLDKSKANDLT 212 
XP_002055003  162 IWTQITSYVAi----------aVDVNEVHLFNVFDAMAELMEHIVIFNEL-TEQSNLSNKWSFYKKWLQTLAKNNEIFQH 230 
XP_015795193  246 QayDhQKMKVLHSLTVQIEKEIIDGNLFSQIIDA--------------------IKSTSSESSpdqliicdHLALYIKNA 305 
XP_001602448  211 P--E-ARILTYEKLLKDLHVQLLSKNLFLKAIEHvm-----------------dVNKSTTMCD--------HMLCFLKNT 262 
XP_624277     210 N--E-SKLHVFNKLLKDLQENLLKKVLFSKTIESvm-----------------dVNKGPIMSE--------HITNYLKNL 261 
XP_019883503  212 A--E-AKLETFDHLLKDLQEKLLRRNMFLKAIEHvm-----------------dVNKGLVMSE--------QIVSYLKNT 263 
XP_002431643  222 I--P-EKLSMLENITKTLESNLLSGKIFHNMLEKlsq--------------isqIVRQSKLGD--------EFLLYLKNM 276 
ESO00575      213 N--S-DQMQQFIVMLNRVDNVVMQGSMFQTCINQkfeep----------efsinVSTNKHFSD--------ECMAAVKEL 271 
EFA80426      245 A--EeERLFQLEKLLLSLKGQLLEGYIFQSCVQQefdfp-----------gvvdVRNNKQLKA--------EFSLNVKSI 303 
XP_001020690  255 D--P-KTVKRFEKNIARLDKTVLSGACVQAVLEQnydanksftsdqlkqqnlclLAKNKDLIE--------LFENYFKQQ 323 
XP_002067587  213 GlyTkLEIGGLYASLDDLE-TVISGNIFRILLDSll-----------------eLKKQFNLKDvnh--ltqHSNAYTRKL 272 
XP_002055003  231 C--TkFEIDGLETSLDDIE-TVITGNIFRIFLQSll-----------------dTKRQLNLKDanvnyitqHSNAYIRKL 290 
XP_015795193  306 VQFHE---GNTNPIk---dDKKWLAINALLAIYVRTSK-REDRKLIKMVFDSQKKINAlyIHLKGNCTIFPDKFLNKQLL 378 
XP_001602448  263 ITDVEskiNDNAAI-----NKNWIQVNVGIVVLIKLFG-TCDKKLLKRIVEVNKKLYA--VTLVGNIVWLPNDFLVDCLP 334 
XP_624277     262 IIDIEnkpNDYVSL-----NKNWIEINIGIVIITKLFG-TCDKRLIKRVLENNKKFYA--VNLIGNIIWIPSKFLSDFLP 333 
XP_019883503  264 ITDVEtksNDYAAL-----NKNWIRANVGIVVVIKLFG-ACDRRLIKRVLETNKRFYA--VTLVGNVIWVPSRFLSNIVP 335 
XP_002431643  277 LTRLEgkdLDMNQMmmnhqEKLWSKVICLFVLNTYLFG-LHDKKLLKQIWEVSKKNVC--INLFKNIIWFPDDFLFQHLS 353 
ESO00575      272 WSRVDqkiGEVSEMn---eRARLIGVLCLFVLHQSVFQ-SQDKKMFKTLFDVHKKIPC--LVLTGSSVFYPSEFLARMLP 345 
EFA80426      304 FGLLSakiMDSSELy---lREKFPGMLGLYTFYISIFKdITDKSFFKQVWELTKRVPM--VSIYNNVIWYPADFIATLMP 378 
XP_001020690  324 VVFYNdilDTTSEVq---eRKDLLYFLCTYAFFRKVFPeEQDKKIYKSLWTLQKKSPV--LEGHTHIFCYVYKFL-RYVC 397 
XP_002067587  273 QLEIEs-rHQANEYrnyeePKHIIRLTAFVAILYQLGI-QIEGKLMKHLLETLSKYHR--LPINQNVHWSPNVFLMQHAK 348 
XP_002055003  291 MSSIEv--nQANEYknyeePKHLLRLTAFICVLHKLGI-LLDGKLMKNALDTLNRYQR--VPLSRNVYWSPHAFICKHAK 365 
XP_015795193  379 KSrv----dkKMIEYFKQTRESTLK---IDLTEKVKSLNIKVNLWMLKFD------------DLLSEiEETTn------- 432 
XP_001602448  335 RE--------GITAIPTQVGEKLLTgriQRLPLVVSNLIQRAMMWGIEMQ------------TLLIK-TGLQ-------- 385 
XP_624277     334 KE--------AGNTINQQVGEKILCsriQKLSQTVNSLIQKAVTWCIDMQ------------SILMK-TSL--------- 383 
XP_019883503  336 KE--------TGTAASPQIGEKILQartQRLPTIVNNLIHRATTWCVEMQ------------SILTR-TNL--------- 385 
XP_002431643  354 HIfka--ddkKQKQSIVNLRNEHLNqkdANLFNQVQKYLQETCLWVIQLN------------SVLKK-DK-Gn------- 410 
ESO00575      346 KLnqmv-vdkKTLQAMESAKLLNLQkmvQCLNLQLPAFEAGLAQWIVKLEgylmslpssltsSSVQL-SDp--------- 414 
EFA80426      379 GMiki--vgnPD---IQGARREYLKivdKEFAQRVKSYYLQVSRWMVRME------------SGSLN-NRg--------- 431 
XP_001020690  398 PLsksttldpKEPEQFTKL---YVQdldIQIPKKINTFYEDFCYWISRME------------SILTSnTALAtgdkskrd 462 
XP_002067587  349 SLirtp-gnkVPTSL--QPNAVSLEkikQNDQRACRQLGTQIAIWSLNMQ------------------RVFAa------- 400 
XP_002055003  366 TLvka--qdkVQDCL--KQYYASMEkinANDLRNCRQLGNQIALWSINMQ------------------RVFAa------- 416 
XP_015795193  433 ----dFIEILHKQIKVIEEGLDLALQCSSIGKNHIYQHNYAKKSLCKSDLIAVCKLTAILKGIELSFLRRKGEILQILAR 508 
XP_001602448  386 -----IFSEIERKRNLLIEILLLMRQIRENVSFVTNLHAFLLKPMSRSTVQLICRLIEVQKSLETTFYTLASTVVLSQSQ 460 
XP_624277     384 -----QISDIGQKQSLLVDLVALLSQIKEIISFIINMHAILIKPMNRNTVQLICRLIEVQKSLQTTFYILGPVIVQSQSQ 458 
XP_019883503  386 -----QVSDIGQKRTLLMDLLALLSQMRETVAFVTNLHASLSKPMSRNTVQFICRIIELQKSLQTTFYALGPIVVQSQAQ 460 
XP_002431643  411 ----lILDELKEKCKLFLAGSKLASDMSRVLKTVLNLHTALSKPMTKATVLYLCKLIELLKSIEMIYQNYYFFMCEPLNH 486 
ESO00575      415 ----rFNEELIEKCKLLQQGLIQSFYLRNTSATLLTTHATLNRPMTKSLVNNICHVVEMLKVIEGSWYRFSIQVVNTASY 490 
EFA80426      432 ----gFWDANLSRIGQIIQGILLSYHISHLFKTMIGLHMSLTAPLKPSDVRRLFQCAELLKSIQGTFHRRTAMISAHISL 507 
XP_001020690  463 rsikiVEEYTSYRVRLLIKALQMASQIKQMITTNLMLHINEGIGFTPQLIKYVVKGIVMLKAIESVYISKIEAFQPHM-- 540 
XP_002067587  401 ----gIFGHLKSFSELILSGQSYADQVAGHIEGLVNRHVALMVPLTKTDFYTICKLMQYLKVIHHTFASNQISFIRFLSS 476 
XP_002055003  417 ----gTFGQLKSFSKLLLLGQGYAAQVNGLIDSLIKRHLALGLSISKVTLFTICKLMQYLQILQKTFANNQILCIRFLSS 492 
XP_015795193  509 STQYYSYLILGALTSCKKRLiSEVKKYSEKKL---DVLSAIILGSNCIHGPIlTLERQILASLCFSIA-----NQSMNEN 580 
XP_001602448  461 VLQQLTYQILTILETVRKSLtQKDKSYSRERL---DALSLIGSSMRLINGPA-TADRRLILRCALVCAsq--lTESFKEE 534 
XP_624277     459 VLQYLSYYILITLETARKSLiQKDKGYSKEKL---DSLSLVGLSMRLLNGPP-SADRRLIIRCALACAnq--lTDTFKEE 532 
XP_019883503  461 VLQYLAYYILAMLETARKGLvQKDKGYSRERL---DALSLVGLGMRLISGPA-SADRRLIVRCTLACAsq--lTDTFREE 534 
XP_002431643  487 IRQYLAYLALNTIKNAKSRI-LSDKSYTKKHL---DTLSALVLSEKVLLGPV-TVERCLIARLGLS-IargnaSKALKDE 560 
ESO00575      491 LVQYFSLLMAVVISNANKRV-RGDKKV---RP---EVLSAFELMLQCLAGPM-TNERILAMKLALG-Fcv--kNKAFKDD 559 
EFA80426      508 MTQQLTTIITNKLDVVRARY-TGKSNYSDTEL---DVIMALTLTSDLLTGVV-TPERLIIIKLCLN-Viy--qANLLKDP 579 
XP_001020690  541 LLKTLVKQLLLILGATYEKL--TKADYKQKKPlynEFRAAIQNVIKNLRGPI-TSLKISTMEFALNFIi---sNYITEKK 614 
XP_002067587  477 LSQWQKQKVLHLLLTTKKKI--VDLKLLQRKM---NILSSLKLTEKSLRSHP-VRRRLTVINLALSEFle--kDRSLPAD 548 
XP_002055003  493 LLQWQKQKMQHLLLLTKKSI--VDLKLMQRKM---NILYTLKLSEKSIKGYP-TKRRLTIVNLALSEYlg--kDRILPAD 564 
XP_015795193  581 ELAKVYSGFKRIRSYCLIFKRLQDW 605 
XP_001602448  535 EVVKLRYYLDTYDTIVELYDLVLET 559 
XP_624277     533 DMVKLRFSLNNYDIIAELHNIIIDL 557 
XP_019883503  535 DVVKLRFLLDSYDAVAELHELVDEL 559 
XP_002431643  561 EVEQIYLNLQLLNKICLLQKTVTES 585 
ESO00575      560 ELVSIRVYLEDIDRFTNFKTLIQES 584 
EFA80426      580 DMEELRLHVKRLEFVSDIHASIRRV 604 
XP_001020690  615 EGQDIKFIFWKIKTVSQFQYNISKA 639 
XP_002067587  549 KQKLFKSIYQRANNLGDIQRNISGQ 573 
XP_002055003  565 KQKLLKSIALRANNLCRLQQNMLGQ 589 
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