1KZP,1FT2,1QBQ,1TN8


Conserved Protein Domain Family
FTase

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cd02893: FTase 
Click on image for an interactive view with Cn3D
Protein farnesyltransferase (FTase)_like proteins containing the protein prenyltransferase (PTase) domain, beta subunit (alpha 6 - alpha 6 barrel fold). FTases are a subgroup of PTase family of lipid-modifying enzymes. PTases catalyze the carboxyl-terminal lipidation of Ras, Rab, and several other cellular signal transduction proteins, facilitating membrane associations and specific protein-protein interactions. These proteins are heterodimers of alpha and beta subunits. Both subunits are required for catalytic activity. Prenyltransferases employ a Zn2+ ion to alkylate a thiol group catalyzing the formation of thioether linkages between cysteine residues at or near the C-terminus of protein acceptors and the C1 atom of isoprenoid lipids. Ftase attaches a 15-carbon farnesyl group to the cysteine within the C-terminal CaaX motif of substrate proteins when X is Ala, Met, Ser, Cys or Gln. Protein farnesylation has been shown to play critical roles in a variety of cellular processes including Ras/mitogen activated protein kinase signaling pathways in mammals and, abscisic acid signal transduction in Arabidopsis.
Statistics
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PSSM-Id: 239223
Aligned: 29 rows
Threshold Bit Score: 338.826
Created: 26-Aug-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:active site cavity [active site]
Evidence:
  • Structure:1KZP; Protein farnesyltransferase (FTase) complexed with a farnesylated Ras peptide product, contacts at 4A
  • Citation:PMID 9065406
  • Comment:The Zn2+-activated thiolate of Cys acts as a nucleophile to attack the ionized farnesyl group.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                            #  ##  #   #                                             
1KZP_B     75 REKHFHYLKRglrql---tdayECLDASRPWLCYWILHSlellde---pipqivATDVCQFLElcq--------spdGGF 140 Norway rat
XP_710548 166 IDAHLKYILSslidp--mpsgyQVLDVNHSWMIYWLLNSyyliqnptmeinqsiLDLIVNKITkcinygdslsgvpfDGI 243 Candida albicans S...
O13782     31 LQKHLKYLTKmldpl---pspfTVLDASRAWMVYWELSSlailgk----ldssvCERAISSVRqlk--------gpsGGF 95  fission yeast
CAG81022   67 TEKHVNFLNMflspdaplpdkfTGLDASRAWLLYWSANAirvlgg---elsdeqKKGIPLTLEsfk---------edGVF 134 Yarrowia lipolytic...
AAN87033   63 KQEHTQFLAStffql---pgkfVSLDASRPWLIFWTVHSldllgv---aldqgtKDRVVSTLLhfl--------spmGGF 128 Cryptococcus neofo...
P22007     80 KEFHKMYLDVafeis--lppqmTALDASQPWMLYWIANSlkvmdrd--wlsddtKRKIVDKLFtis--------psgGPF 147 baker's yeast
CAG98113   79 KDSHKMFLEYwlnnp--lpsgfKSLDASQPWLLYWIGNAfktmnpt--wltndyQKRILDKLWyis--------ptgGPF 146 Kluyveromyces lact...
CAG90775  100 ITEHNKYIMKsltdp--lpyyyKSLDANHGWLLFWLINSysiingr--lkdakiRDLVSNKICrnvi------degsGGI 169 Debaryomyces hanse...
EAA55636  110 RKKHVRFLRNmlrql---papfIAADASRPWFLYWSLNAmailge---nvkedyAESLADTARsmq--------nesGGF 175 Magnaporthe grisea...
XP_761495  88 RQAHISFLTKllepl---pgayTAFDTNRSWLLYWILHSydllsv---sldpkgRARAIATLLsfqn-------kgsGGF 154 Ustilago maydis 521
Feature 1               # ## ##                                                               
1KZP_B    141 GGG--PGQYPHLAPTYAAVNALciigtee-------------------aynvinREKLLQYLYSL-----KQPDGSFLMH 194 Norway rat
XP_710548 244 GGG--NNQLGHLASTYAAILTLiltdqy--------------------elldnlRELIRDWLLTLkkrssCGSGASFIMH 301 Candida albicans S...
O13782     96 CGG--NGQDEHLLSTYASILSIclcdstd-------------------ayslieRDRLYDWLFSL-----KNPDGSFRVN 149 fission yeast
CAG81022  135 GGG--SGQDAHAASTYAAFLALadsdded------------------awgrlinPEEVLKHNLKL-----KSPDGGFASN 189 Yarrowia lipolytic...
AAN87033  129 AGGpaNSQIPHLLPTYASVCSLaitgndgpt--------------ggwkdladaRQSLYEFFMRC-----KRPDGGFVVC 189 Cryptococcus neofo...
P22007    148 GGG--PGQLSHLASTYAAINALslcdnid------------------gcwdridRKGIYQWLISL-----KEPNGGFKTC 202 baker's yeast
CAG98113  147 SGG--KHQLPHLAATYAAINSIalchnld-------------------dnreinKKAIYDWLISL-----KTPSGAFMTA 200 Kluyveromyces lact...
CAG90775  170 AGG--ANQIGHAAATYASILALvlvedy--------------------ellnkiRPNLYKWFLSL-----KMPNGSFIMH 222 Debaryomyces hanse...
EAA55636  176 SGG--HGQTSHLATTYAVVLALavvgdee-------------------glslidRRALWKWLCDL-----KEADGGFRMS 229 Magnaporthe grisea...
XP_761495 155 GGG--PDQIAHLMATYAAVSALaiiggpgpaptaehvadgksvevghggwdaidRTTMYNWISSL-----KQPDGSFLVH 227 Ustilago maydis 521
Feature 1             #  ##                                                                   
1KZP_B    195 -VGGEVDVRSAYCAASVASLTnii--------------tpdLFEGTAEWIARCQNWEGGIG------------------- 240 Norway rat
XP_710548 302 -ENGEMDARSTYCALIIINLLnltnyeens---sspeeldpLIDGVENWLNSCQTYEGGFS------------------- 358 Candida albicans S...
O13782    150 -NEGESDARSVYAAVCVSSLVgism-------------ddpLFEGTLQWLCKCQTYEGGLS------------------- 196 fission yeast
CAG81022  190 -VGGETDVRGTYCRLVVASLTntl--------------tteLTDGVIKYIASCQSYEGGFG------------------- 235 Yarrowia lipolytic...
AAN87033  190 -EGGEVDVRGTYCLLVVATLLdii--------------tpeLLHNVDKFVSACQTYEGGFAcasfpfpsvvpstsalpts 254 Cryptococcus neofo...
P22007    203 lEVGEVDTRGIYCALSIATLLnil--------------teeLTEGVLNYLKNCQNYEGGFGs------------------ 250 baker's yeast
CAG98113  201 rPVGEQDVRGVYTALSIASLLgiv--------------dskLTSNVTEFLTRCQSYEGGFG------------------- 247 Kluyveromyces lact...
CAG90775  223 -QNGESDTRSTYCVLVVASLLnil--------------ttdLCEGTLQWLNLCQTFEGGFA------------------- 268 Debaryomyces hanse...
EAA55636  230 -LGGEEDVRGAYCAAVIISLLnlpldlckdseayirdptanLFTGLGDYVRKCQTFEGGIS------------------- 289 Magnaporthe grisea...
XP_761495 228 -VNGEVDVRAGYCVICITTLLgis--------------tpkLFDGMAPFIASCQTYEGGIAaasqptyqasadddilrvs 292 Ustilago maydis 521
Feature 1                # #   #                                                              
1KZP_B    241 ----GVPGMEAHGGYTFCGLAALVILkker---------------------------slnLKSLLQWVTSRQMrf--eGG 287 Norway rat
XP_710548 359 ----NIPNTEAHGGYTYCALASYFLLyenrkqfssgst-----------ssssrsrsnidWEKLLEWSVHRQHel--kGG 421 Candida albicans S...
O13782    197 ----GVPYAEAHGGYTFCALAAIALLggld---------------------------nlnEIKLSTWLVQRQDpa--lYG 243 fission yeast
CAG81022  236 ----GSPGNEAHAGYTYCALAALAILvpirem-----------------------dqyvnVESCLAWLSARQYqp--eGG 286 Yarrowia lipolytic...
AAN87033  255 epscRVSMAEAHGGYTSCSLNSHFLLtsvplps---------------------fpssidASAALRWTVLQQGeaiegGG 313 Cryptococcus neofo...
P22007    251 ----CPHVDEAHGGYTFCATASLAILrsmd---------------------------qinVEKLLEWSSARQLqe--eRG 297 baker's yeast
CAG98113  248 ----GCPNDEAHGGYTFCAVASLAMLnald---------------------------kvnIDALLSWCSSRQTke--eKG 294 Kluyveromyces lact...
CAG90775  269 ----GVPNTEAHGGYTFCGMASYFLLlnpmngdfl-----------------qqinnqidVHLLIRWCVMRQYql--eGG 325 Debaryomyces hanse...
EAA55636  290 ----GQPDAEAHGAYAFCALGCLSLLgtpset----------------------ipkylnIERLISWLSSRQYap--eGG 341 Magnaporthe grisea...
XP_761495 293 qdvaRPPLGEAHGGYTYCAAASSLSLslldsslggsttaasssadfkvvsssheptaqldRDALIRWATAQQGipfegCG 372 Ustilago maydis 521
Feature 1              # ##  #                                                                
1KZP_B    288 FQGRCNKLVDGCYSFWQAGLLPLlhralhaqgdp---------------------------------------------- 321 Norway rat
XP_710548 422 VDGRTNKLVDACYGFWMGGLFSLlqliimdfsqg---------------------------------------------- 455 Candida albicans S...
O13782    244 FSGRSNKLVDGCYSWWVGASHVIvasgygsash----------------------------------------------- 276 fission yeast
CAG81022  287 FSGRTNKLVDACYAYWVGASLVLingavha-------------------------------------------------- 316 Yarrowia lipolytic...
AAN87033  314 FRGRTNKLVDGCYSWWVGGGAPVaeelvrrekskkvkksrveli------------------------------------ 357 Cryptococcus neofo...
P22007    298 FCGRSNKLVDGCYSFWVGGSAAIleafgy--------------------------------------------------- 326 baker's yeast
CAG98113  295 LNGRSNKLTDGCYSFWVGGTAAIleaygy--------------------------------------------------- 323 Kluyveromyces lact...
CAG90775  326 LSGRTNKLVDACYSFWIGAIYPMielitn--------------------------------------------------- 354 Debaryomyces hanse...
EAA55636  342 FSGRTNKLVDGCYSHWVGGCWPLieaclngpvkvs--------------------------------------------- 376 Magnaporthe grisea...
XP_761495 373 FRGRTNKLVDGCYGWFSGGGLFTilsamieadlieqarpsssaatssavevhdwngmltvpatpvilapsssssnssgsw 452 Ustilago maydis 521
Feature 1                                                                #        ##          
1KZP_B    322 --------------------------alsmshwmfhQQALQEYILm---CCQCPAGGLLDKPGKSRDFYHTCYCLSGLSI 372 Norway rat
XP_710548 456 -------------------------qgqqqevkvfdEEKLRQYLLi---IAQDESGGFKDKPGKQVDYYHTNYSLSGLSI 507 Candida albicans S...
O13782    277 ---------------------------kslpnlfynPEKLLGYILq---CCQSTSGGLRDKPPKRPDQYHTCYCLLGLSS 326 fission yeast
CAG81022  317 ------------------------------gpslwdRKQLAQYVLn---CCQQSGGGLRDKPGCKADAYHTNYAACGIAM 363 Yarrowia lipolytic...
AAN87033  358 ----------------eeeekegdwedvppipptfnRVALQEFTLvaaqQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSI 421 Cryptococcus neofo...
P22007    327 -------------------------------gqcfnKHALRDYILy--cCQEKEQPGLRDKPGAHSDFYHTNYCLLGLAV 373 baker's yeast
CAG98113  324 -------------------------------gvcidKDALKQYILk--cCQSEESPGLRDKPGTQADFYHTNYVLAGLSI 370 Kluyveromyces lact...
CAG90775  355 ------------------------------tktifdRDALKCYILn--cCQNIETGGFKDKPGKSVDFYHTNYTICGLSI 402 Debaryomyces hanse...
EAA55636  377 -------------------------sldvepqplfsREGLMRYILcc-cQEQGKRGGLRDKPGKPSDAYHSCYVLSGLSS 430 Magnaporthe grisea...
XP_761495 453 rtepsldagvandcgydsndatavdiedlspltlfdRVGLQEYILvaaqRTASDGGGLRDKPGKRPDAYHTCYNLSGLSL 532 Ustilago maydis 521
Feature 1      
1KZP_B    373 A 373 Norway rat
XP_710548 508 L 508 Candida albicans SC5314
O13782    327 I 327 fission yeast
CAG81022  364 S 364 Yarrowia lipolytica CLIB99
AAN87033  422 A 422 Cryptococcus neoformans var. neoformans
P22007    374 A 374 baker's yeast
CAG98113  371 C 371 Kluyveromyces lactis NRRL Y-1140
CAG90775  403 T 403 Debaryomyces hansenii CBS767
EAA55636  431 A 431 Magnaporthe grisea 70-15
XP_761495 533 S 533 Ustilago maydis 521

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