3CKX,3GGF,2XIK,3A7H


Conserved Protein Domain Family
STKc_MST3_like

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cd06609: STKc_MST3_like 
Click on image for an interactive view with Cn3D
Catalytic domain of Mammalian Ste20-like protein kinase 3-like Serine/Threonine Kinases
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily is composed of MST3, MST4, STK25, Schizosaccharomyces pombe Nak1 and Sid1, Saccharomyces cerevisiae sporulation-specific protein 1 (SPS1), and related proteins. Nak1 is required by fission yeast for polarizing the tips of actin cytoskeleton and is involved in cell growth, cell separation, cell morphology and cell-cycle progression. Sid1 is a component in the septation initiation network (SIN) signaling pathway, and plays a role in cytokinesis. SPS1 plays a role in regulating proteins required for spore wall formation. MST4 plays a role in mitogen-activated protein kinase (MAPK) signaling during cytoskeletal rearrangement, morphogenesis, and apoptosis. MST3 phosphorylates the STK NDR and may play a role in cell cycle progression and cell morphology. STK25 may play a role in the regulation of cell migration and polarization. The MST3-like subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270786
Aligned: 24 rows
Threshold Bit Score: 438.602
Created: 2-Aug-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:3A7H; Human MST3 binds ATP; contacts at 4A.
  • Structure:3CKX; Human MST3 binds the inhibitor staurosporine; defined at 4A contacts.
  • Structure:3GGF; Human MST4 binds Cd ion and quinazolin; contacts at 4A.
  • Comment:Also based on the binding of ATP analogs or inhibitors to other STE family kinases

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  #####   #            # #                #             #                
3CKX_A          7 ELFTKL-EKIGKGSFGEVFKGIDnrtqKVVAIKIIDLEeaeDEIEDIQQEITVLSQCd-SPYVTKYYGSYLkd--tkLWI 82   human
1174431        16 KLYSIQ-SCIGRGNFGDVYKAVDrvtqEIVAIKVVNLEhsdEDIELLAQEIFFLAELk-SPLITNYIATMLed--vsMWI 91   baker's yeast
729897         21 SLFKRT-EVIGRGKFGVVYKGYNvktgRVYAIKVLNLDsdsDEVEDVQREIQFLASLkqISNITRYYGSYLkd--tsLWI 97   baker's yeast
XP_001324427    8 SDFQRC-QMIGKGNFGLVFKAIYkktgQTVAIKEIDLEesdDDLTEIQREIDMLRACe-SPFVIRYEGCVLvg--skLWI 83   Trichomonas v...
XP_503033      56 DQFELL-EELGSGSFGVVYKAIDkvsgQIVAVKKIDLEsseDDIEEIQKEIAILSGCq-DEHITTYYGCFVrg--ykLWI 131  Yarrowia lipo...
O14305          7 NSYTLL-RKLGSGSFGVVWKAREnvsgDIIAIKQIDLEtgiDDITDIEQEVFMLSNCn-SSNVIQYYGCFVdg--ytLWI 82   Schizosacchar...
CAB16374        4 HQYRDL-QLIGQGSFGSVFRAQDvessKIVALKVVDLDatkDQIETLTQEINFLIDLn-SVHITKYYASFVdg--frLWI 79   fission yeast
XP_502534      80 DEVVALdVVLGKGSFGKVYKGRIrktnQVCAVKQVNLDe-eDDIDEIALEVNFLASLr-CPFITEYYGSVVdnrdytLWI 157  Yarrowia lipo...
XP_712526      10 EELEVY-EEVGRGGFGVVYRGIIkstnQEVAIKQIDLEkdsTDLIEINKEIQIISECh-CQQITSYMGSFVkn--ykLWV 85   Candida albic...
XP_001484294    3 NKYCLE-KCIGRGNFGDVYKAACkrsgKVVAVKVVNLDeslEDMFFMVQEIQFLSLMr-SPYIVQYLESFVen--snMWI 78   Pichia guilli...
Feature 1          ####  ##  #                                         ## #           #           
3CKX_A         83 IMEYLgGGSALDLLEpg---pLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSe-hGEVKLADFGVAGQLTDTq 158  human
1174431        92 VMEYCgGGSCSDLLKrsyvngLPEEKVSFIIHEVTLGLKYLHEQRKIHRDIKAANILLNe-eGMVKLGDFGVSGHIRST- 169  baker's yeast
729897         98 IMEHCaGGSLRSLLRpg---kIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITn-eGNVKLCDFGVAAQVNQTs 173  baker's yeast
XP_001324427   84 VMEYMgAGSVRDLILir---kMPETAIAIVLNQILQGLDFLHRGRKVHRDIKAANILLSn-eGDVKLADFGVASSLESR- 158  Trichomonas v...
XP_503033     132 IMEYLaGGSGLDLLKpg---iFHEPEIAVMCRELLEGLIYLHDNGKIHRDVKAANVLVSs-eGSVKLADFGVATQLSNNm 207  Yarrowia lipo...
O14305         83 LMEHMdGGSVSGLLKmg---rLNEQVISIILREVLYGLNYLHGQNKIHRDIKAANILLSsstGNVKLADFGVAAQLSNAa 159  Schizosacchar...
CAB16374       80 TMEYCdGGSCLDLLKls--gtFSERVIAEVMRQVLEALVYLHGQGKMHRDIKAANILTMk-dGLVKLADFGVSGQLESLr 156  fission yeast
XP_502534     158 LMEYCgGGSCADLIKsq---pFREDHIAVVMRDTLRGLEYLHKQRKVHRDIKAANILLTt-eGDVKLADFGVSGEMGLSi 233  Yarrowia lipo...
XP_712526      86 IMEFIdGGSIFELLKpg--piDDEKIVSFILYEILLALNYLHNQGKIHRDLKSQNILISk-tGDIKLTDFGVSTQLSSNf 162  Candida albic...
XP_001484294   79 IMEYCgGGSVADVLRyc--kkFDENIAAYIIKYVLLGLSYLHKQKTVHRDVKLANVLVTt-dGTIKLADFGVSGEMSFTt 155  Pichia guilli...
Feature 1                                                                                         
3CKX_A        159 IKRNXFVGTPFWMAPEVIKQSa--YDSKADIWSLGITAIELArGEPPHSELh-PMKVLFLIPKn-----NPPTLEGN--- 227  human
1174431       170 LKRDTFVGTPYWMAPEVVCCEvdgYNEKADIWSLGITTYELLkGLPPLSKYd-PMKVMTNLPKr-----KPPKLQGP--- 240  baker's yeast
729897        174 LRRQTMAGTPYWMAPEVIMEGv-yYDTKVDIWSLGITTYEIAtGNPPYCDVe-ALRAMQLIIKs-----KPPRLEDRs-- 244  baker's yeast
XP_001324427  159 CRAFTFVGTPFWMAPEVITEGg--YDEKCDLWSLGITAIEIAtGSPPLAELh-PQRVLILIPQn-----APPQLQGD--- 227  Trichomonas v...
XP_503033     208 SRRNTFVGTPFWMAPEVIRQEd--YDTKADIWSLGITAIEFAkGEPPLSEYh-PMKVLFLIPKa-----EPPTVPAGg-n 278  Yarrowia lipo...
O14305        160 SRRHTFVGTPFWMAPEVIQQTs--YGLAADIWSLGITAIEMAnGIPPRATMh-PMRVIFEIPQs-----EPPKLDDH--- 228  Schizosacchar...
CAB16374      157 DKNDDFVGTPFWMAPEVVKQTg--YNYKADIWSLGITAYELAtGEPPYSGIh-PMKVLLLIPKh-----SPPSLERSk-- 226  fission yeast
XP_502534     234 KKMRSFVGTPYWMAPEVISKAg--YDYKADIWSLGITALELAdGSPPLSGLs-PMKALSVIPDr-----ECPSLPHGn-q 304  Yarrowia lipo...
XP_712526     163 SKRNTTVGTPYWMAPEVIVNNnggHSYKADIWSLGCCCYEMFtGKPPLQSKypPMRALRLISKcqknqdFIKLIELNeld 242  Candida albic...
XP_001484294  156 NKRSTFVGTPYWMAPEVIVHSklgYNEKADIWSTGITTIELIkGDPPLADYd-PMKILFKIPKq-----EPPLLTGDk-- 227  Pichia guilli...
Feature 1                                                              
3CKX_A        228 YSKPLKEFVEACLNKePSFRPTAKELLKHKFILRnakktsyltELIDRYKRWK 280  human
1174431       241 FSDAAKDFVAGCLVKtPADRPSAYNLLSFEFVKNitit--nlkSDVDLIKQKK 291  baker's yeast
729897        245 YSTSLKEFIALCLDEdPKERLSADDLLKSKFIRAhkat--ptsILKELISRYL 295  baker's yeast
XP_001324427  228 FSPQFKDFVEKCLIKdPAIRPSARDLLNHPFVKNakk----rdALIQYIDNVR 276  Trichomonas vaginalis G3
XP_503033     279 WSADFRDFVACCLRKnPAERPSGRQLLKHRFIRKagkt-sqlkLLVDRRERWM 330  Yarrowia lipolytica CLIB122
O14305        229 FSPTFRDFVSCCLDLnPNMRWSAKELLQHPFIKSagtvkdiipLLVQKENKLF 281  Schizosaccharomyces pombe
CAB16374      227 FSRAFCDFVSNCLKKnPKDRATAEYLSKHKFIKKycpn-tsvkEVVASYAKWK 278  fission yeast
XP_502534     305 FTELFREFIDACMCKdPDSRSSATQLLTLKFVRTtkrp-silvPLIQQHHKFV 356  Yarrowia lipolytica CLIB122
XP_712526     243 ISWEFKDFLHQCFIEdPKLRASASKLLKHKFITKhihdkdkikLLKKLITKKQ 295  Candida albicans SC5314
XP_001484294  228 YSDELKGFLEKCLVKnPRKRSSSTELLDHPFIGIaaeqqdfckLLRRRRTSFL 280  Pichia guilliermondii ATCC 6260

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