6LCR


Conserved Protein Domain Family
CDC50

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pfam03381: CDC50 
LEM3 (ligand-effect modulator 3) family / CDC50 family
Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.
Statistics
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PSSM-Id: 460906
Aligned: 290 rows
Threshold Bit Score: 151.213
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6LCR_B        68 LTYLAHTVRDLKIDYT--------------------------DCLTSAPKddfetipqnhitahfsakdstfdpykaqwk 121 Chaetomium ther...
XP_648055     52 VFVVTNNCQEYSVKYVgeg--------------------salTCKQgat------------------------------- 80 
CBZ12968      53 LFRANASAVDITVRYDhq-----------------------qQCSFGYNNtgafry------------------------ 85 
Q38DJ5        42 VEFCNRNAKEASFRYDne----------------------ptKCTEETFSlig--------------------------- 72  Trypanosoma brucei
Q4DU99        47 VTLMNKQAKEVTVRYDhi-----------------------hRCTITHNTgafiy------------------------- 78  Trypanosoma cruzi
EGT57938      84 LAAANQQVILIRFQYDnit------------------------------------------------------------- 102
Q4CMM3        52 VIKASDSVFELSIRYD-------------------------------ETNnyqyrvgpddk------------------- 81  Trypanosoma cruzi
CAM65930     166 VLVKGREYHIVEQEYShihqyqyvpsnssvninhgilqftadGVTHAQGT------------------------------ 215
Q382L6        78 LFITCNGHYEVRGNYQhihkyqyipsdpkvninegirsfyvgNEVHRQGT------------------------------ 127 Trypanosoma brucei
XP_001562722 170 VLVKGRQQHIIEQEYShihqyqyvpsntsvninqgilqftadGVTHAQGT------------------------------ 219
6LCR_B       122 tterevqvanyTDNrqf--ciVRFNIPEDL---------------QPTISFFYYLENFYQNHRRYVNSFNAKQLLGDAvd 184 Chaetomium ther...
XP_648055     81 -------------------ceFQFNIPKPM---------------KTPVYVYYQLTNFYQNHREYLRSRSNKQLKGDPis 126
CBZ12968      86 ------easpgDVWqtgcvtdVPFRVDKHL---------------KAPVYVYYGLENFYQNHRRFSNSKSDAQLAGQRv- 143
Q38DJ5        73 --------gqgTGKrtvcethFEFVLAETL---------------RQPVYFYYGLTKMYQNHRRYTNSRSGKQLMGADv- 128 Trypanosoma brucei
Q4DU99        79 -------egnnMTFktgcmteVSFDITEKL---------------KAPVYLYYELTRFYQNHRRYSISRNDEQLAGKAv- 135 Trypanosoma cruzi
EGT57938     103 ------------------ngyIDINITNFIpvsrflncsfsiffaQAPVYFYYELRNTFQMHRSLNQAYCKRQLVf---- 160
Q4CMM3        82 yphkfrfnnsnFSTgah--vsRNFTLLKSL---------------ASPIYLQYALVGFYQNYRRYAFSRDLAQREGNAy- 143 Trypanosoma cruzi
CAM65930     216 -----------RTW-------LEINISHRM---------------KAPVYMYYKIANMHQNHRDFHNGRSNSQLRGKSk- 261
Q382L6       128 -----------RTR-------VSFKLEKPM---------------KKPVYLYYTLGNFHQNFRAFHEGRSLDMLRGHRs- 173 Trypanosoma brucei
XP_001562722 220 -----------RTW-------LEINISRGI---------------LAPVYMYYKISNMHQNHRDFHRGRSNSQLRGKSt- 265
6LCR_B       185 g-ktiN-DSt--CDPI--------THDpk--------------gTGKIVYPCGLVANSIFNDTF--------------SS 224 Chaetomium ther...
XP_648055    127 tysqlSd-----CTPLislnnsknPH--------------------MFYEPCGLVAASFFNDSFeit--------mqpEK 173
CBZ12968     144 ----sAaAIasaTSPLtypgelrhTADqginll-----gtflhySDFVYVPAGLIPWSMFNDTFtly--------ritHH 206
Q38DJ5       129 -------RSetdANPFvipgdtmdNTNkpiefg-----grnhtyKDFVYVPVGLVAWSMFNDTFtlf--------rkeKN 188 Trypanosoma brucei
Q4DU99       136 -------RYlpdTSPLaipgdiygISGtpikyvd----gsdlryKDFLYVPAGLIAWSIFNDTFtly--------teaTN 196 Trypanosoma cruzi
EGT57938     161 -------GDstgCDSFknskyaceNPSpsstflag--fstfcanGQKFYAPVGGISSIMFNDYF--------------K- 216
Q4CMM3       144 -------LVsaaCEPFrfpgeyqnRKI------------------LGIYFPCGSIAWSLFNDSFsly--------rlsDA 190 Trypanosoma cruzi
CAM65930     262 -----IdKAy-lCKPYtypgfrsdEGDtpititdvtgtqvtrpaRYFTYNPCGIAPWSKFNDTFilyrkltpaevvqaNI 335
Q382L6       174 -----IiGSypeCQPYerpgtinkAEKtevkvvvd-genvtlkyEEFLYNPCGIAPWSMFNDTFvlyrsrdvssaqndSV 247 Trypanosoma brucei
XP_001562722 266 -----IdKTy-tCQPYtypgfrnnKGEtpititdatgrqvtrpaRYFTYNPCGIAPWSKFNDTFvlyrkltpaevlqaSI 339
6LCR_B       225 PLALAVRn---------------sSDss--------rPYNM-TTKGIAW----------------PGLK-D--------- 254 Chaetomium ther...
XP_648055    174 ESSSVL------------------L--------------EL-NKENINW----------------KSDK-K--------- 194
CBZ12968     207 EAAAVTApslrli------cngsaFSrftn---epldGAGRcHKKGIAW----------------TSDVeF--------- 252
Q38DJ5       189 GSNPGEVlicng-------tdfsrHTnkpl---hrsvSHNHcDKNGIAW----------------ESDIkK--------- 233 Trypanosoma brucei
Q4DU99       197 GGTSRKLicnat-------dfskgNNlpln----gseSKNMcVKKGIAW----------------DTDVeY--------- 240 Trypanosoma cruzi
EGT57938     217 --L------------------------------------TL-NNTEISWteegvivdkrretffqPEDSdN--------- 248
Q4CMM3       191 SKSTINSqdfena-----klicdgGAfdaag--kslnEKNLcIKKGIAL----------------SSDVrLfhpltdgkk 247 Trypanosoma cruzi
CAM65930     336 SGVPVLHggvdsttpvtlicngtdFGlrgeplsgsvtKNHC-SKMGISW----------------KADReI--------- 389
Q382L6       248 KLDEGAElicnt-------sdfgpTGeply---qsktPNKC-KKKGISW----------------PADEkI--------- 291 Trypanosoma brucei
XP_001562722 340 SGVPVLHggvdgttpvelicngtdFGlkgepldgsvaVNRC-SKMGISW----------------KADRnI--------- 393
6LCR_B       255 -----LYG-K---------------------T--SY--SL-DQIVPPPnwerrykygyq------ennpppdlktDELFQ 296 Chaetomium ther...
XP_648055    195 -----LFGe-------------------------------------PAerngik---------------vvnsytDPDFI 217
CBZ12968     253 -----KYKkPhfpppss-------prpvwsaPkwAYeaADgDVNPNPPsrmpsdnayfnegwyadepghripvttDEDLM 320
Q38DJ5       234 -----KFLePkwdgsg---------pvwtapRs-EY--------GKPSiesndtyfnngwy--ageeghmipvvtDEDFM 288 Trypanosoma brucei
Q4DU99       241 -----KFKaPdleakn---------rfwtaaKe-LYt----GKVPTPElsnddffnkgwy---agelghaipvttDEDFM 298 Trypanosoma cruzi
EGT57938     249 -----LCDa-----------------------------KEfRNTVKPIgwnqhic--------------emggyrNISFI 280
Q4CMM3       248 dsavwRFGg------------------------------------DPAandpyqkegyyy----eepghpipsnvDEDYI 287 Trypanosoma cruzi
CAM65930     390 -----RFRnItlredwwslyypypttneylrNg-WYlhEPgHALPDPS---------------------------DYDLH 436
Q382L6       292 -----RFRpLerdkklwslryphkndnvyltNg-WYadEPgHRLTDPE---------------------------DYDMQ 338 Trypanosoma brucei
XP_001562722 394 -----RFHnMtlredwwslyypypttneylrNg-WYldEPgHSLPDPS---------------------------DYDLQ 440
6LCR_B       297 NWMMLAAAPNFYKLYQK-Ndt--hPMLAGQYEIEIES-----NFDVTVYKGRKAFVITTLSTMGSRNIWPGIIFLIVGGI 368 Chaetomium ther...
XP_648055    218 NWMRPAVSSTFRKLTGIiEn---vEEVKGNVTVKVVN-----NFPVESFKGTKTIILATTSVFGSKNPALGIIYMATGGV 289
CBZ12968     321 VWARVASLPKFRKLYRViDe----DLVPGTYLMRIQE-----HFNAASYGGTKSFSLATLSWLGGRNTFMAWMYFTIGAV 391
Q38DJ5       289 VWMRASPLPNVRKLYRIiRt----DLRAGKYVMKIRQ-----NYNTKPYGGEKSFIFLQPSMLGGKLTSLSITYFAVGGL 359 Trypanosoma brucei
Q4DU99       299 VWMRPASLPSFRKLHRViNv----DLPPGKYVMVIGE-----HFDVSSFGGTKSFALATLSFLGGKNVWLEALYFSLGGF 369 Trypanosoma cruzi
EGT57938     281 KWLEPSTNKNFKKLYRIlDsskhdGLRRGVYRLYIDNvynpgEFKSKEFKLEKYFWILHPSWFGTEQKFLEIMYLIVGAG 360
Q4CMM3       288 VWSSLSYMKDFTKKYRIiTt----DLVPGNYLIDIVE-----NFDVFSFSGEKYVSLVTRSWIGGKNYVLGILFLLMGCI 358 Trypanosoma cruzi
CAM65930     437 VWMRASFTSNFRKLYRIiHv----PLHPGTYLVDISE-----FYDVVSFRGRKSVVLQHANWVGGRNIVLGVVFIIMGCA 507
Q382L6       339 VWIRAAFLSNFSKLFRIiDe----DLREGNYFLDIEE-----FFDVTTFHGTKGYLLRTSSMFGRSGTLFAATFLIVGSV 409 Trypanosoma brucei
XP_001562722 441 VWMRASFTSNFRKLYRIiDm----PLYPGTYLVDISE-----FYDVVSFRGRKSVVLQHTNWIGGPNIGLGVIFILMGCL 511
6LCR_B       369 CLVLDIYFILSFFIwRPRK 387 Chaetomium thermophilum var. thermophilum DSM 1495
XP_648055    290 FVIIAILLFILTRV-SPRK 307
CBZ12968     392 SAVSGASFLCIHHWyGDRA 410
Q38DJ5       360 ALIFTVLVLFASHVwGHHS 378 Trypanosoma brucei
Q4DU99       370 SVVFAVVLLLVHRFsGDRA 388 Trypanosoma cruzi
EGT57938     361 LLAFSCFLVGFQIFlMDRR 379
Q4CMM3       359 SFVLSLSFIIVQYLhSKRC 377 Trypanosoma cruzi
CAM65930     508 SFVLGVTFTVECMLqLNGV 526
Q382L6       410 AFVVGVAFAIQYCM-AKKG 427 Trypanosoma brucei
XP_001562722 512 SFILGVTFTVECLLqLNGV 530
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