Conserved Protein Domain Family
SARAF

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pfam06682: SARAF 
SOCE-associated regulatory factor of calcium homoeostasis
SARAF is as family of eukaryotic proteins embedded in the ER. SARAF is SOCE-associated regulatory factor, where SOCE is store operated calcium entry. ie a mechanism governing calcium homoeostasis in the cell and the mitochondria. SOCE involves the enabling of Ca2+ release-activated Ca2+ (CRAC) channels. SARAF is a single pass ER membrane protein whose systolic-facing domain is responsible for activity and whose luminary-facing domain carries out a regulatory function in conjunction with another membrane protein STIM, an ER single pass membrane protein that detects changes in ER Ca2+ levels through its EF-hand, conserved Ca2+ binding domain. STIM is the major target for SARAF regulation, and thus SARAF negatively regulates the SOCE entry of calcium into cells protecting them from overfilling.
Statistics
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PSSM-Id: 461985
Aligned: 45 rows
Threshold Bit Score: 235.698
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EGF82781      18 LLFLVCILAPTATAAShKKVRLTNIQALTLHAGRMTTARRSSPVKQLSCVnsgnsgffSsskSACSDtallPQTVQCINK 97 
4_pfamImport   1 LQFLL--VGHIKSWDD-GSVLLRDLQALTLYKNRYTTARRASPVPQLQCVg-------GsagCQAFV----PEVVQCQNK 66 
XP_015199412   7 IFVLFIYTGRTESWNDgGSVLLRELQVITLYKGQYTNSRRTSPVPQLQCIg-------GtagCGSFI----PEVVQCHNK 75 
XP_006010005   8 LFVLSLMLGPADGWNShESVLLQEVQVLTLYKNRFTTARRTSPVPQLQCNg-------GtagCSAFV----SEVVQCQNK 76 
F7C1E2        14 PLLALLQIPGAWCWNHqERVLLRDIQAITLYADRYTNARRSAPVPQLKCIg-------GsagCHTMV----PQVVQCHNR 82  tropical clawed...
XP_003772841  24 VLLLLPRPSPALAWDQsEKILLRDIQALTLHRDRFTTARRTAPIPQLQCLg-------GsagCPAHI----PEIVQCRNK 92 
XP_003797715  18 LLLLLLIAGPALGRNDpDRILLRDVKALTLHYDRYTTSRRLDPIPQLKCVg-------GtagCDAYT----PKVIQCQNK 86 
Q6AYN2        19 LLSFLLVAGPALCWKNpDRILLRDVEALTLYSDRYTTSRRLDPIPQLKCVg-------GtagCDAYT----PKVVQCQNK 87  Norway rat
Q08E24        18 LLLLLLVVGTAQCWDErDRILLRDIKALTLYYDRYTTSRRLEPIPQLKCVg-------GtagCDSYT----PKVIQCQNR 86  cattle
XP_014309083  17 GLLLLLIAGPALGWNDpNRILLRDIQALTLHYDRYTTSRRLNPIPQLRCIg-------GtagCDTYT----PKVIQCQNK 85  little brown bat
EGF82781      98 GTDGVDVQWACTAELDSTVKFGSeMVVACEGWAYPEDPFVLAGSCGLEYTLVRTALYDRTRPSKEHHRFGEKWkpswsfw 177
4_pfamImport  67 GWDGVDVQWECRTDMDNAYRFGR-IEVSCEGYSHPADALILKGSCGLEYTLELTEHGRSTSHGGTKSFGGRRid------ 139
XP_015199412  76 GWDGFDVQWECKTDMDYSYRFGK-IEVSCEGYNYPDDPYVLKGSCGLEYTLELTEEGKQKGRNSRSfgsgffqd------ 148
XP_006010005  77 GWDGFDVQWECKTDMDNNYRFGT-IQVNCEGYNSPDDPYVLKGSCGLEYTLELTEAGRMKRNKGYGSGGfssnffkd--- 152
F7C1E2        83 GWDGFDVQWECKVDMDNSYRFGK-VEVSCEGFDYPEDPYVLRGSCGLEYTLELTEEGRKRSQDGYRSGGfgsgyfqs--- 158 tropical clawed...
XP_003772841  93 GWDGFDVQWECKAELDTSYRFGK-TAVSCEGYDYPDDPYVLRGSCGLEFNLELTEQGRKKYKDAGSSNTNSHGntygsny 171
XP_003797715  87 GWDGYDVQWECKTDLDVAYKFGK-TVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLKKFKESGKHHsfaafsdy---- 161
Q6AYN2        88 GWDGYDVQWECKTDLDIAYKFGK-TVVSCEGYDSSEDQYILRGSCGLEYNLDYTELGLSKLKESGKHQsfsdyy------ 160 Norway rat
Q08E24        87 GWDGYDVQWECKTDLDVAYKFGK-TVVSCEGYESSEDQYVLRGSCGLEYQLDYTELGLKKLRESGKEHgfnsfsny---- 161 cattle
XP_014309083  86 GWDGYDVQWECKTDLDVAYKFGK-TVVSCEGYESSEDQYVLRGSCGLEYDLDYTELGLKKLRESGKNNgfnpfs------ 158 little brown bat
EGF82781     178 kskehyytentDGSSWIGFIIWTAVFAFIAYRISLACCskprrqgnhrtteTRGTFHSTAHAGtsf--------dpnSGP 249
4_pfamImport 140 ---------daKSR-----LLVVAVLLLVAYGVYKLFL-------------SGEPRPPWGRtvdrqvtpgiiahgstAAP 192
XP_015199412 149 ---sssnvnyqGSGGDVSGLMVVAVLLLLAYGVYKMFLcgp--------hgQGHQRFPDAdfsnantd-ghftdgntMGP 216
XP_006010005 153 ---fsnnvnspVSSDSSGVL-VVVILLVIAYGIYKIFLss----------pQSEQPFPGSTGDfntdrqnyyssshsMGP 218
F7C1E2       159 ---nsnswdtkTNGSSA---IVLIVIVIIAYGVYKLFLsg---------psVQEHPRPDEYGYdfqs------qtraSAP 217 tropical clawed...
XP_003772841 172 fpgyvekmdspVPS-STRGLLAVVILLVLALGVYKLFLkaqned--lpppyTEHPQDhqrff----------qasppPPP 238
XP_003797715 162 ----ynkwysaDAC-STGGLITIVVLLTIAFAVYKLFLsdgqc---spppySEYPPFshhyq----------rftnsAGP 223
Q6AYN2       161 -----hklssvDSC----GLVTVAVLFVLAFVVYKLFLsdgqg---spppySEHPPYsqhsq----------rfagtAGA 218 Norway rat
Q08E24       162 ----ysklyspESS-SLGGVVTIVVLLAIAFGVYKFFLsdghe---spppySEDPPYshryq----------rfsssAGP 223 cattle
XP_014309083 159 ------dyyksNSS-GISGLITIVVLLAIAFGVYKLFLgggqd---spppySESPPYspgfq----------gftnsAGP 218 little brown bat
EGF82781     250 PPPYndgwesqdnth---qhasaphpgaYpssgggnSQNSdGSRSNNDGFWSGFTSGSILGSLFGNRSYWYPSTNYFGYG 326
4_pfamImport 193 PPPGfkpdftgnpgsnp------gygfhsdytdrqqYPGGhATPGTGGGFWTGMGTGGMLGYLFGHNRSVKRERERRIHD 266
XP_015199412 217 PPPGfksyytgespgyesa-------rdsygfkntftrphqKPASG-PGFWTGMGTGGVLGYLFGSQR------------ 276
XP_006010005 219 SPPGfkpdfaghstdgegfsggfnfgnyD-------TRHRhNGNSG-PGFWTGLGTGGILGYMFGNQRSN--QSPY---- 284
F7C1E2       218 PPPGfksdftdphlckifg----------asksfssgygtshsNQG-PGFWTGLGTGGVLGYLFGNRRAQPYQAPY---- 282 tropical clawed...
XP_003772841 239 PPPGfksyftasgtpg-------------wgfgnsftgpqaFGQSG-PGFWTGLGTGGLLGYLFGNNRA----TPL---- 296
XP_003797715 224 PPAGfkseftgpqntgrg---------atsgfgsaftgqqgHENSG-PGFWTGLGTGGILGYLFGSNRA---ATPF---- 286
Q6AYN2       219 PPPGfksdftgpqstsyg----------assgfgsafggqsyASSG-PGFWSGLGAGGLLGYLFGSNRA---ATPF---- 280 Norway rat
Q08E24       224 PPAGfkseftgphgat-------------agfgsaftgqqgHEHSG-PGFWTGLGTGGILGYLFGSNRA---ATPF---- 282 cattle
XP_014309083 219 PPPGfkpgftgtpgat-------------ygfgsaftgqqgNANSG-PGFWTGLGTGGILGYLFGSNRAT--TTPF---- 278 little brown bat
EGF82781     327 pqaypsytfypprptswwswGSGWPQSTy----T------YSnpmfrrtssssgsrsqspaPSTRTSTTYGGTRRR 392
4_pfamImport 267 ynle---------------------------------knrANnydhspysrhppaagytssPGTRTASGFGGTKRR 309
XP_015199412 277 ----------------------------------------SQpryftrntgnsnigptpvnSGTRTVSGFGGTKRR 312
XP_006010005 285 --------------------TNIWGNPAypppmHgypsan---------------rgvsesTGTRTASGFGGTKRR 325
F7C1E2       283 --------------------FNTWTGPSn----Sdsmhqn---------------yrppqsSGVRTASGFGGTKRR 319 tropical clawed frog
XP_003772841 297 --------------------SNNWTHPAypppyFntwnnpVppsapvnsr--ssgsssgsdTGTRTTSGFAATKRR 350
XP_003797715 287 --------------------SDSWYYPAcpppySgtwngrASsplrgssgg--ysacanaeTKTRTASGYGGTRRR 340
Q6AYN2       281 --------------------SGSWYHPSypssyAgawnshAYsplgggagr--ysassnteSRTRTTSGYGGTRRR 334 Norway rat
Q08E24       283 --------------------SDSWY-PSyppsyTstwnsrAYsplrggpgs--ssacsgsePRTRTASGYGGTRRR 335 cattle
XP_014309083 279 --------------------SDSWYRPSyp-syFgtgnsrASsphcgdsgn--ysacsssqTKTRTASGYGGTKRR 331 little brown bat
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