Conserved Protein Domain Family
MAD

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pfam05557: MAD 
Mitotic checkpoint protein
This family consists of several eukaryotic mitotic checkpoint (Mitotic arrest deficient or MAD) proteins. The mitotic spindle checkpoint monitors proper attachment of the bipolar spindle to the kinetochores of aligned sister chromatids and causes a cell cycle arrest in prometaphase when failures occur. Multiple components of the mitotic spindle checkpoint have been identified in yeast and higher eukaryotes. In S.cerevisiae, the existence of a Mad1-dependent complex containing Mad2, Mad3, Bub3 and Cdc20 has been demonstrated.
Statistics
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PSSM-Id: 461677
Aligned: 13 rows
Threshold Bit Score: 525.846
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
ADK12653             53 AAERVRSHTSLIQLTQEKQQMELSHKRARIELEKEahsssrdLQREIDRNRDLQMKIRRLEERE-EKANQALNEQMENNK 131
P87169               54 NPKLASLEFQLENLKNDLKRKELEFEREQIELQRK-------LAEEHEQKNSLQLRLTLVEKQL-EEQSTSYQKEIEEVR 125 Schizosa...
XP_013025869         41 SSRVQSLEFALENAKNDLKQKEIESDRERNDLLAK-------LTEEREQSKAFQIRVSLLEKQE-HEREATNHETIERLR 112
WGS:AAQB:GK15834-PA  83 RAELIESKAIVSQLQRDMEREAKEYRSELAISESKaaalkeqCDYTSAKMQELEKHLQVVRKREqVASESASRANVELAQ 162
XP_002050330         85 RAELIESKAIVSQLQDQLAQQAKEQRSALALAESKatalkqqCDYTSAKLLEQDKQLQVVRKREhNARQEANRAMAEMAQ 164
Q7KND8               80 RTELIETKAIVIQLRNEIEKKSREHKEAILLAENKstalkdqCDITSKKNLELQDDLKALRKRElVLKNEASRATAELNQ 159 fruit fly
XP_001959874         79 RADIIELKARNSQLQHEVERQIKEHRRAMAVAESKstalkqqCDITSKKMIEMERNVQIMRKREvALKEEACSARAELAQ 158
EDW36037             82 RAELIETKARLSQVQDQLKQNETKRRMELSLAESKvsalktqCDFTSQKLLDLTKDMENRRGLEnSYKEEARRAKAELAG 161
XP_005054501         54 QAGQIHSKSQLLQVEREKMQMELSHKRARIELEKAantnarnYEREADRNQELLTRIKQYQERE-AEAENKLKEQMEMNK 132
Q9WTX8               54 RAEQIRSKSYLIQVEREKMQMELSHKRARVELERAastnarnYEREVDRNQELLARIRQLQECE-ATAEEKMREQLERHR 132 house mouse
NP_001095514         54 RAEQIRSKSHLIQVEREKMQMELSHKRARVELERAastsarnYEREVDRNQELLTRIRQLQERE-AEAQEKMKEQLERHR 132
XP_006637049         53 EAQKIFSKTHLLQMAQEKSQMEFSHKRARIELEKAahtsardLEREVDRNQDLLARIRKLEEKE-SEAGKALKEQTEISR 131
XP_018558114         52 AAEQVYSKNRYLQLDQEKKQMEMSHKRARFELEKAasdsardLEHEIDRNQELLGRIKKLEERE-TEAAKNLSEQVEANR 130 barramun...
ADK12653            132 alkrnleelhkkaNEKDSKLTEAEQIISELKDETRRLGQQIQIQESKFSTQNLEIQALQEQLDVQRKKFQDISQKY---- 207
P87169              126 -------------NEKEATQVKIHELLDAKWKEIAELKTQIEKNDQALSEKNHEVMVSNQALQMKDTNLTNLEKLFadsr 192 Schizosa...
XP_013025869        113 -------------SDNESLVLQLSELKSNLTQETAARATELEKTQQELREKIYKVEDFQQRNKLLEENASQMEIRNsslq 179
WGS:AAQB:GK15834-PA 163 qktkyeaiinqleKTKMQQEGDARDVHLCIGNELNEYRRLAQRANLELQSTRNELERIQRRHDEYKARISNYEELK---- 238
XP_002050330        165 qklkyetalsraeQEKTQLEEDAREVQHCINNELSEYKRISERAELELEATQSELERIRERHDEYKATAANYEQLQ---- 240
Q7KND8              160 lrlkfdestlklqKEKYLQKEDARDVHLCINNELSEYRRIAQRADLELQSTRNELERLRQLNEELQARASGFEQLR---- 235 fruit fly
XP_001959874        159 lkmkfddsidslqKEKSQQEEDARDVQLCINNDLAEYRRMAHRADLELQATRTELERFRRRHDEFKARVSGHEDLR---- 234
EDW36037            162 lklkydetvnklkNEKSQQEQDARDVQLCINNELGDYRRQAQRAELELQSTLKELECIRQRHDEYKARMSGYEELR---- 237
XP_005054501        133 sykksmetmskkiQEKESKLAEANETITVLKGKISELQWNIMNQEMQMTSQDSEKQELMEQLDIEKKKWQEASQQI---- 208
Q9WTX8              133 lckqnldavsqqlREQEDSLASAREMISSLKGRVSELQLSAMDQKVQVKRLESEKQELKEQLELQQRKWQEANQKI---- 208 house mouse
NP_001095514        133 lcqqsldaagrklREKEDGLAEAGETITTLKGRLSDLQWSMMNQEMQVKRLESEKQELKEQLDLQHQKWQEANQKV---- 208
XP_006637049        132 slkktiegqnkklEEKDSKLAEANETSRALRDEIRELHHKIKNQEIQLTTQTTENQGLQEQLELLHKKCQEATQKI---- 207
XP_018558114        131 alrknleglnkklEERDSRLNTANQTVSSLKDEIRELKQKIQNKDSTISTQTLENQELQEQLDLQRRKYQEVSQVC---- 206 barramun...
ADK12653            208 QNLQSSHSTSTESELKMKELERRLAL------QEQDSVIVKNMKSEV-ARLPEMEREIKRLREENSFLRETKENNSLLKE 280
P87169              193 EQLETKCKELAAAEQQLQELSVHNQQleesikQVSSSIELEKINAEQrLQISELEKLKAAQEERIEKLSSNNRNVEILKE 272 Schizosa...
XP_013025869        180 EQLNSKLKESLDKEQQVQTLIFEIEKlkesvnQASDSLELKDANQKLlERIAELEKLQEKHLYMIERYNTNSRNIEIVNE 259
WGS:AAQB:GK15834-PA 239 SNYEKQQHSLKTANERIKELEYEIQS------YNDWKEVTKASQDRL-ASIPDMQAELDRLRGHNKHLNSLIGDKLLLEE 311
XP_002050330        241 MSYEMQQQTLATANARIKELEYEIQS------YADWKEITKTSQARL-LSVPELQSELERLRAHNKQLNSLIGDKLLLEE 313
Q7KND8              236 ANHEKQTQSLKVANDRIQELEFEIQS------YSDWKEVVKTSRERL-ASVPDLLAEVEHLRSHNKHLNTLIGDKLLLEE 308 fruit fly
XP_001959874        235 SDFEKQSQSLKAANERIKELEYEIQS------YNDWKEVTKASQERL-ASVPDLQSEIERLRHHNKHLNTLIGDKLLLEE 307
EDW36037            238 ANFEKQQQSLKVADERIKELEFEIQS------YTDWKQVTKVSQERL-ASIPEMDSELQRLRVHNKQLNKIIGDKLLLEE 310
XP_005054501        209 QTLQASQSLLAEYEQKIKDLEQKLSQ------QEHDALIVKNMKAEL-ARFPKMERELRQLKEENAYFREMKENNGLLKE 281
Q9WTX8              209 QELQASQDERAEHEQKIKDLEQKLCL------QEQDAAVVKSMKSEL-MRMPRMERELKRLHEENTHLREMKETNGLLTE 281 house mouse
NP_001095514        209 QELQASQEVRADQEQRIKDLEQKLSL------QEQDAAIVKNMRSEL-VRLPTMERELRQLREENACLRETQDTNGLLRE 281
XP_006637049        208 QALQMYPSLNSENELKIKELERRLAM------QEQDSVIVRNMKSEL-GRIPDMEKELQQLREENAYLRETKENNNLLKE 280
XP_018558114        207 QNLQAAQSSCSEHIIKIKELERRLAL------QEQDIAIVKTVKSEV-ARVPDLEKELKRLRDDNAFLRESRENCSLLKE 279 barramun...
ADK12653            281 ETEGLRRKLERLEKVMEEKIKVELEKEKMERELQAWENIGQAtg--lNIRKPEDLSREVIQIQQRELNFKQQNYSLNSSV 358
P87169              273 EKNDLESKLYRFEEYRDKVATLELENEKIQTELNSWKSLITN-----ELPTPEAVSNKLVFLQNTNANLGERVSSLESQL 347 Schizosa...
XP_013025869        260 EKADLEAKLYQYEDYKEKVAVLGVDNEKLQAELNSWKKTFM------DMNVPELAAEKFKTLQSENKTLSERVSDLTQQL 333
WGS:AAQB:GK15834-PA 312 QVHHYKTRLEKEEGARAEAASLQVKLSHVEQELKEWVKIAQDhclanTLVSPIALRARIEQLLQEDIVHVAEKTSSESDS 391
XP_002050330        314 QVHDYKTRLDREEGARAEAATLQVKLTHVEQELKEWVKVAQDhclpnTLVSPMALRTRIEQLLQDDIVRMAEKGSADSDS 393
Q7KND8              309 QVYDYKTRLEREEGARAEAASLQVKLLHMEQELKEWVKVAQDhclanTLVSPMALRSRIEQLLKEDIIHVAEKTSSASDT 388 fruit fly
XP_001959874        308 KVHDYKTRLDREEGARAEAVSLQVKLSHVEQELKEWVKVAQDhclpnTLVSPMALRSRIEQLLQEDIVHVAEKTTSESDT 387
EDW36037            311 QVHEYKARLDKEEGSRAEAAALQVKLSHTERELKEWVKVAQDhclanTLVSPVTLRSRIEQLLQGDIVHVSEKYASESES 390
XP_005054501        282 EVEGLQRKLERYEKVQAQLVTVELENEKLLGKLQSWEKLDQStg--lNIRTPDDLSRQIVALQQRELALKEQNSTFMNSA 359
Q9WTX8              282 ELEGLQRKLSRQEKMQEALVDLELEKEKLLAKLQSWENLDQTmg--lNLRTPEDLSRFVVELQQRELTLKEKNNSITSSA 359 house mouse
NP_001095514        282 ELEGLQRRLGRQEKMQETLVDLELEKERLLTKLQSWERLDQTtg--lSIRTPEDLSRFIVELQQRELALKDKNSAITSSA 359
XP_006637049        281 EAEGMRKKLERVEKMKEDLVKLELEKEKLSLKLQAWQNLEQMtg--lNIRRPEDLSGEIIQFQQREITLKQQNYSVTSSA 358
XP_018558114        280 EVEGLRRKLERMEKTKEELVNMELDKERLAEKLQAWENLGQStg--lNIRKPEDLSREVIQIQQREIALKEQNYTLNSRV 357 barramun...
ADK12653            359 RSVEKARSELLTEIAQLraKAQEEQKKRENQDSLVRRLQKRVLLLTKERDGMRAILESYDSE---LATSEYSPQLTLRVK 435
P87169              348 SNKPANQPLGANEKDAA--HITELETKLKELHEQNRRLQRQKSLATQEIDLLRENLKSYDDEeaiLSEKNTDMKKLERIE 425 Schizosa...
XP_013025869        334 LQERIQGPPMVIELKN---KVTDLKNENKETSEQIRRLQRQVFLANQEINLLRANLKSYDDEeaiMNNEKSGQKKLERIE 410
WGS:AAQB:GK15834-PA 392 KHLHSTIKDLEQKCAAYlkNIEDLNIGLKRHKNFKERLQRKLIIVSKERDFYKQLLENFDKD---QTLNNTS-------- 460
XP_002050330        394 KQLQSSLRELEQKCAVYlkNIEDMNVVLKRHKSFRERLQRKLLIVCKERDFYKQLLENFDKD---LNMTNAS-------- 462
Q7KND8              389 KHLNTTIRDLEHKCAIYlkNIEDLNIGLKRHKNFKERLQRKLITVSKERDFYKQLVENFDKD---TTLSNAS-------- 457 fruit fly
XP_001959874        388 KHLQATVRELEQKCSVYlkNIEDLNIGLKRHKNFKERLQRKLITVSKERDFYKQMVENFDKD---LTMSNTS-------- 456
EDW36037            391 KQMRNLVRDLEQKIRVYlkNIEDLNMSLKRHKNFKDRLQRKLRTVSRERDFYKQMVDNFDKD---LTMSNAS-------- 459
XP_005054501        360 RMLEKARQQLQEEILSVqsQLLDEKKKREHQEALVRRLQKRVVLLTKERDGMRAILESYDSE---LSPAEHSPQLSRRMR 436
Q9WTX8              360 RGLEKVQQQLQDEVRQAnaQLLEERKKRETHEALARRLQKRNALLTKERDGMRAILGSYDSE---LTQTEYSTQLTQRLW 436 house mouse
NP_001095514        360 RELEKARLQLQDEVRQLggQLLEERKRRETHEALARRLQKRVLLLTKERDGMRAILGSYDSE---LTPAEHSPQLTRRMR 436
XP_006637049        359 RALEKVRLELQNELLQLraKLLEEQKRREQQDALVRRLQKRVLLLTKERDGVRAILESYDNE---LTASEYSPQLSQRVK 435
XP_018558114        358 RSVERSQTELRAELSQQrsKTLEEQKKRESQDALVRRLQKRVLLLTKERDGMRAILESYDSE---LAPTEYSPQLSKRLR 434 barramun...
ADK12653            436 EAEDMLQKVQAHNAEMETQLSKAQeeagsfklqaQMVAAELEALKeQQVSNAERSSsvtaeeISSLRQKIEELEAERQR- 514
P87169              426 GLVKLVDEYKLKLESMPVSLDVDE----------TSDEVSLQKRR-RKNEHKDAGY------VTELYRKNQHLLFQVKEk 488 Schizosa...
XP_013025869        411 SLTQLIDEYKVLFENSKIynq-----------gaSADEQSLTLKRpRNEDQEDVGY------VTELYRENQHIQLQLQEk 473
WGS:AAQB:GK15834-PA 461 -AADMTQDMH-----VRHRIEVLErtvtgykdicATLEREIQAMR-QQEQLHEPtn----egYDSVKKELDTLRLENER- 528
XP_002050330        463 -TAEMTNDMQ-----VRYRVEVLErtltgykemcSTLEREMQSMR-QQESLNDPPise--ngYDSVKRELDTLRMENER- 532
Q7KND8              458 -VADMTQDMQ-----VRVRMEVLErtvtgykdmcATLEREIQSLR-QQELVNEPag----egYDSVKKELDTLRMENDR- 525 fruit fly
XP_001959874        457 -MADMTLDMQ-----VRYRMEVLErtvtgykdmcATLEREIQAMR-QQEQLAEPsg----egYDSVKKELDTLRLENDR- 524
EDW36037            460 -VAEMTQDMQ-----VRYRVDVLErtltgykdlcTTLDREIQAMR-EQEQLSDPss----edYENVKKELDTLRLENDR- 527
XP_005054501        437 EAEDMVQKLHAHNAELEAQLSQVLeevgshkqraEMLEVEMKVLK-SQQCTAEQSTvvtkeeVDTLRLKIEELEAERSK- 514
Q9WTX8              437 EAEDMVQKVHAHSSEMEAQLSQALeelgvqkqraDTLEMELKMLK-AQTSSAESSFsfckeeVDALRLKVEELEGERSR- 514 house mouse
NP_001095514        437 EAEDMMQKVQAHSSEVEAQLSQALeelggqkqraDMLEMELKILK-SQAAPAEQSFlfsreeVSSLRLKVEELEAERSR- 514
XP_006637049        436 EAEEMLQKVQTHNSEMEAQLSKAQeeagayklqaQVAEAEIDILK-KQAATADNSSfvsteeVNSLRQKIEELEAERRR- 513
XP_018558114        435 EAEDVLQKTQNHNAEMEAQLTKAQeetgtlrlqlQTVELELDSLKkQQASAADSSSlatkeeVSILRQKIEDLEAERQR- 513 barramun...
ADK12653            515 ------LEEQNNILEMRLE--RHNLQGDYDPVKTKVVHLQMNPTSMAKQQRADEVEQLRVECQRLRDRLRkievag---- 582
P87169              489 tnieafLREQIITLESSIAtlRQELAQVTEINSCRVLQHRSNPTLKYERIKAAQLEMLNAENSALKALLE---------- 558 Schizosa...
XP_013025869        474 lntegfLRDQINGLEVIIAelRQEIIQMSELKSSKVLQHRSNPTLKHERVKAEQLHLLEKENGGYKAILE---------- 543
WGS:AAQB:GK15834-PA 529 ------LRRRKDELELEMV--NRSLRGT-DTANHKVVHLSQNPAAEAYECSKNMMEKLQAEIERLKRRIKkme------- 592
XP_002050330        533 ------LRRRKDELELELE--HRCLRGDFNMGNYKVLHLSENPAADAYEATKNVVEKLQAEIERLKRRNKkle------- 597
Q7KND8              526 ------LRRRKEELEMEMM--HRCLRGDFNMKDFKVVHFSENPAAEAYESTKNMMEKLQAEIERLKRRNKkle------- 590 fruit fly
XP_001959874        525 ------LRRRKEEMELELM--HRCLRGDFNMKEFKVVHLSENPAAEAYESSKNVMEKLQAEIERLKRRNKkle------- 589
EDW36037            528 ------LRRRKEEQELEIM--QRCLRQDISPPISKVVHLVDNPAAEAYESSKNMLEKLQAEIERLKRHNKkle------- 592
XP_005054501        515 ------LAEENRSLEMKLE--KLTLQGDYDPSRTKVLHLSMNPMSLAKQQRKEEQQQLQEECERLRELVRvlkggg---- 582
Q9WTX8              515 ------LEQEKQVLEMQME--KLTLQGDYNQSRTKVLHMSLNPISMARQRQHEDHDRLQEECERLRGLVHalergg---- 582 house mouse
NP_001095514        515 ------LEEEKKTLEAQLE--RLTLQGDYDQSKTKVLHLSQNPAGAARQRLREDQQQLQEECARLRELVRaleagg---- 582
XP_006637049        514 ------LEEQNNMLEMRLE--RHNLQGDYDPTKVKVLHFRMNPASQARQQRAQDAEQLRQELQRLKEHIQllqagg---- 581
XP_018558114        514 ------LEEQNNVLEMRLE--RHNLQGDYDPVKTRVLHLKMNPTAVAKQQRQQEVEALREEVTHLRELVRslqdggslvh 585 barramun...
ADK12653            583 ---------------gmttdD-TTLIIPPS--------------QEILDLRKQMESAELKNQRLKEVFQKKIQEFRTACY 632
P87169              559 --------------------DkKVDCLPIQsfk--------iaeRKALDLKKEVAEREKRIQRLKEIFSVKSLEFREAVF 610 Schizosa...
XP_013025869        544 --------------------EkDIQTVPMEsle--------lanRRTLELKKELLDRDKRIQRLKEIFSVKSLEFREAIY 595
WGS:AAQB:GK15834-PA 593 -----------------edsEq-----ATTtditsasagmtmnfKELNQMRADLESANAKMRKMKDCFKAAGKEFRDVVY 650
XP_002050330        598 -----------------edqEqTQARFNETvgsssagggmtmnfKEFNQLRAELESANGKMKKMKDCFKAASKEFRDVCY 660
Q7KND8              591 -----------------ddnEq---RLNETtstgg----mtlnfKEFNQLQAELESANGKMRKMRDCFKAAREEFRDVCY 646 fruit fly
XP_001959874        590 -----------------ddqEq---RLNETtstgg----mtmnfKEFNQLRAELESANGKMRKMKECFKAATTEFRDVCY 645
EDW36037            593 -----------------danEq---HLNETtststg--gmtmnfKELNKLRAELESANAKLGKTKEYFMAARKEFRDVVY 650
XP_005054501        583 --------------slsgelE-GVGAFQSP--------------QEVAELKKQVESAELKNQRLKEVFQTKIQEFRKVCY 633
Q9WTX8              583 --------------pipadlE-AASSLPSS--------------KEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCY 633 house mouse
NP_001095514        583 --------------pvpahlE-AGAGLPSS--------------REVAELKKQVESAELKNQRLKEVFQTKIQEFRKVCY 633
XP_006637049        582 ---------------avaaeE-GPLSLPPS--------------QEVLDLRKQVESAELKNQRLKEIFQKKIQEFRKVCY 631
XP_018558114        586 sqddssmhssslglslppskE-IL------------------------DLRKQMESSELRNQRLKEVFQRKIQEFRTVCY 640 barramun...
ADK12653            633 VLTGYQID-ITVENQYRLTSVYAEHMEDSLLFkstgPVGsGSMQLLETDFSRTLTGLVDLHLFHQKSIPVFLSAVTIELF 711
P87169              611 SLFGYKLD-FMPNGSVRVTSTYSREDNTAFIF----DGEsSTMKLVGNPSGPEFERLIRFWCDERKTIPGMLAALTLELL 685 Schizosa...
XP_013025869        596 SLFGYKIE-FLPNGSVRVTSMYSHENNTAFVF----DGEtSTMKLVGKPSSPDFERLVRFWCDERKTIPGMLAALTLELI 670
WGS:AAQB:GK15834-PA 651 MLLGYRVDrMGANSNYRISSMYAENPTDYFDItlseSNC---LALLESPYSQTLKPAIDQQLAANNSFPAFFSSLTLELF 727
XP_002050330        661 MLLGYRIDrVGANSHYRISSMYAESPDDYLSIslneSNC---LALLESPYSQTLKPAIDQQLAANNSFPAFFAGLTLELF 737
Q7KND8              647 MLLGYRIDrIGANSNYRISSMFAEGPDDYLDIslneSNC---LALLESPYSHTFNPPIDQQLAASN-FPAFFSALTLELF 722 fruit fly
XP_001959874        646 MLLGYRIDrIGANSNYRISSMYAESPEDYLDItlneSNC---LALLESPYSRTLKPAIDQQLAASNSFPAFFSALTLELF 722
EDW36037            651 MLLGYRIDrVGYKSNYRVTSMYAESPDDYFSIalseSND---LALLETPYSETLQPALDQQLAANNSFPPFFSSLTLDLF 727
XP_005054501        634 TLTGYQID-ITTENQYRLSSIYAEHQGDCLLFkassSSG-GKMQLLETEFSRTVRELIELHLLRQDSIPAFLSALTLDLF 711
Q9WTX8              634 TLTGYQID-VTTESQYRLTSRYAEHQTDCLIFkatgPSG-SKMQLLETEFSRSVPELIELHLLQQDSIPAFLSALTIELF 711 house mouse
NP_001095514        634 TLTGYQVD-ITTESQYRLTSMYAEQKADCLIFkaagPSG-TKMQLLETAFSRTVPGLIELHLLQQDSIPAFLSALTLDLF 711
XP_006637049        632 MLTGYQID-ITTENQYRLTSVYTERVEDSLLFkasgPTG-GNMQLLETDFSRTLHDLVELHLFHQKSIPAFLSAVTLELF 709
XP_018558114        641 VLTGYQID-ITTENQYRLTSVYAEHMDDSLLFkk-gSNG-S-MQLMETEFSKTLGEMVALHLHHQKSIPAFLSAVTLDLF 716 barramun...
ADK12653            712 SRQT 715
P87169              686 DKND 689 Schizosaccharomyces pombe 972h-
XP_013025869        671 DKND 674
WGS:AAQB:GK15834-PA 728 QRST 731
XP_002050330        738 QRAT 741
Q7KND8              723 QKAT 726 fruit fly
XP_001959874        723 QRAT 726
EDW36037            728 QRAT 731
XP_005054501        712 SRQT 715
Q9WTX8              712 SRQT 715 house mouse
NP_001095514        712 SRQT 715
XP_006637049        710 SRQT 713
XP_018558114        717 SRQT 720 barramundi perch
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