Conserved Protein Domain Family
Mid1

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pfam12929: Mid1 
Stretch-activated Ca2+-permeable channel component
MID1 is a yeast Saccharomyces cerevisiae gene encoding a plasma membrane protein required for Ca2+ influx induced by the mating pheromone, alpha-factor. Mid1 protein plays a crucial role in supplying Ca2+ during the mating process. Mid1 is composed of 548-amino-acid residues with four hydrophobic regions named H1, H2, H3 and H4, and two cysteine-rich regions (C1 and C2) at the C-terminal. This family contains the H3, H4, C1 and C2 regions. suggesting that H1 is a signal sequence responsible for the alpha-factor-induced Mid1 delivery to the plasma membrane. The region from H1 to H3 is required for the localization of Mid1 in the plasma and ER membranes. Trafficking of Mid1-GFP to the plasma membrane is dependent on the N-glycosylation of Mid1 and the transporter protein Sec12. This findings suggests that the trafficking of Mid1-GFP to the plasma membrane requires a Sec12-dependent pathway from the ER to the Golgi, and that Mid1 is recruited via a Sec6- and Sec7-independent pathway from the Golgi to the plasma membrane.
Statistics
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PSSM-Id: 432882
Aligned: 83 rows
Threshold Bit Score: 261.786
Created: 24-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EME85774     163 IYISANTCTQPvw---nGSEPQTEAPPQLTLYVSTSSGN-TDLGPNA-GNESQsv-----ypFVNGFVNAS-VDnASG-- 229
EAU91143      52 LVLSVAVCSE---------------NSNLRFIVSNSSST-SNPNSDGgAlv----------nLVNGVGTYVsGY-PNG-- 102
CAR26813     100 ILLSGNICKQP----------SNTNGTSLGVFYSFNETlfGD------LGVGKfq------sFGNGYLQALaIS-PLEan 156
XP_002551528  83 VFISGNLHSES-------ARLSNSTELQLAVWYSFDQEvtSD------TSKGSra------lFSAGYMEALaAT-PLEdq 142
Q6CNE5        86 IFLSANICERP--------SK-VDNDTSLVLYYTFNSNdtGT------FTTAKss------tFDDGYLEALaLR-PYYtd 143 Kluyveromyces l...
Q758F9        99 L-LSGNICWRP----------LNTTGQVLRVYYSFNKTtlDD------LSGAEaa------dFAEGYMEALaVR-PYQvd 154 Eremothecium go...
XP_003645827 101 IFLSGNICSQP----------ANVTGQSLRVYYSFDESmvDN------LSIAEaa------dFSLGYMEALaIR-PYEve 157
XP_001644330  85 VFMSGNICSMD--------NITTDGSPSLRIYYGFNESilSD------LTTASyg------dYTEGYFSSYaIS-PFAnd 143
XP_003688713  75 lFISGNLLNIP-----------NTNGSVLRLYYSFSDTifAN------LSDTVye------dFYDGYVSAM-VS-TFSdt 129
XP_004179180  69 LFISGTIESMPlryynlTDNTQFQNDTALQVTYYFSPS----------DPSNStkigynnitFENGYIMALgTS-PITyn 137
EME85774     230 -----------------------DWYMAVSGPSLPDGf---------------SG--SWTYRLAVSIDD-YFYAAeMGDP 268
EAU91143     103 ---------------------------GVLQIEGSGS-----------------------FEVALSDTGqPIHAH--FPP 130
CAR26813     157 nnna--------------tskynNLYVVVTPVNETTGqplps-------spekVNgeRWEYKMSISEND-LVYQW-DSRS 213
XP_002551528 143 dgn---------------lteysNLYLVIEAYNVTSNsplq--------ltstTQdlSWNYTIGVSQYD-LVFQW-DVRS 197
Q6CNE5       144 dssl-------------nngtysTLYLMVQLVNSTTGqpfesd-----gstiadDdeRWSYQISISQRD-LVFQW-DKRP 203 Kluyveromyces l...
Q758F9       155 dnq---------------tdpysTLYLVSLLVDSETGkplp---------aaaANpePWHYRLSISQND-LGFQW-DSRP 208 Eremothecium go...
XP_003645827 158 sns---------------tetysTLYLLVELVDTASGkslp---------asqGSaePWSYRISISQND-LAFQY-DAKT 211
XP_001644330 144 ss-----------------ilysKLYIVAKLFDYDTQmple--------rtpeNEnqFWNYTLSVSLNH-LTYQW-DSRS 196
XP_003688713 130 atdl--------------nttysTLYLALQVVDQETGvalvv-------ngdsNSddTWDYQIAVSEKD-LYYQW-DRKT 186
XP_004179180 138 plfaleeqettnitgqslvleytTLHLEVSLINPQTGeiiqpsdnddyydyedDDdnLWTYHLGVAEDD-QLFQY-DERS 215
EME85774     269 FLFQVDTDSDSALLVTDNLTQADASDplytew-----------mdlgapFIMFAVNNNLSG-MMG--LESSFCGLQTFAs 334
EAU91143     131 LPLLGDTTSNQAILFSPPFFVPEPIQprypnytmlpvdipqdsppqtpdYTLSLFPTREDD------YPRSSCFLSAES- 203
CAR26813     214 WLDVLDTDHTSALLLTGNVS---ADAenhynys----------iynpslYDLYVYSDEESAkLDKs-TNISLCAMIQGN- 278
XP_002551528 198 WVELVDSDADSALLVTGNVT----SLtttsans---------slydvglYELYLYSYDELIaFDKa-LPHSLRAVQDGP- 262
Q6CNE5       204 WLDVVDTDYNSALLVTGNVT---AMSkttsnys----------iydvslYDIYIYGFEYAEyFDNlnLSHSLCAIQNGP- 269 Kluyveromyces l...
Q758F9       209 WIEVIDTDYNSALLSTGNLGPASDDKgkrky--------------dpdlYDLYLYSVEHIDtFNG--LSRSYCAVSGGP- 271 Eremothecium go...
XP_003645827 212 WIDVVDTDHNSALLTTGNMStfspnhgi--------------adidlslYDLLLYSSDHIDqFNN--LSRSICAIANGP- 274
XP_001644330 197 WLQVLDTDYNSALLSIGSSS--VANStkstgv-----------ssalkyYDVYIYDTKEADiIDEm-FGMSVCAIKNGP- 261
XP_003688713 187 WIEVMDTDYNSVLLSIGQFV----TAtsaksns----------tssdeeYVLYIYTPEEAEkITAn-RNCSISAVKNGP- 250
XP_004179180 216 WIHVLDTDHNSALVTSGNDS--TRLTtylktn-----------ntlespYELFLYSLKDSEiIENn-LNCSLSAILNGP- 280
EME85774     335 dnhQIQAKQDdmggnt--------------------tnvqmGMITR-GlgHKPKEQFLVNALNSSSSYHAFLAMDGnsta 393
EAU91143     204 ---QLESGTItse-------------------------etpWLRDEdG----WRLQYLIRGLEPSTNYTAYVL------- 244
CAR26813     279 ---YLVSSAQsppkdvhpl-------------ertelavsrSMADRsG--S-VREQFYVTGLNASTDYVAYLTKKIgkkg 339
XP_002551528 263 ---YLASSVNnsavkiels-------------nsstpeivkSLTVRgG--S-VKEQFYITGLNHSTTYGVYLTKKIsdnt 323
Q6CNE5       270 ---YLISSANgslngtnngntae-----naslssttlsvhkSITVRgG--S-AKEQFHITGLNSSTTYVAYVTKKLatst 338 Kluyveromyces l...
Q758F9       272 ---RLIRSSSsdyterpnst-----------lsnpslkitkSLVEQvG--Q-VREQFHITGLNSSTTYVAYLKMRVssa- 333 Eremothecium go...
XP_003645827 275 ---ILASSLNtvthfenstl------------sstelqitkSITTRgG--Q-IKEQFHITGLNSSTTYLAYLTKKVsnva 336
XP_001644330 262 ---YILSSRDfatlninttml----------nndtlaaqkiSLGTFyG--S-STGQFYITGLNSSTTYSAYLVKKIvdtn 325
XP_003688713 251 ---HIISSENvtesdvdlsll----------dredfqifklNVDAYtS--N-NHGQYYITGLNSSTSYVGFLTQTItgsn 314
XP_004179180 281 ---YMATSISndsnstpstnykkislnvhslqiqkqlvgddQL-------SNVQDMLYISGLAPNTTYALYLIRKIgtrm 350
EME85774     394 tgdgvvgGGGKVWAPVPFSTKs-DGNCQLLFNLTFCDEVAYAVPANPS-Nee-vsSFEALQNFYEDYTTRWYAPFNRTLA 470
EAU91143     245 -------DDTRVFGPIYFTTKsaAFPCHLVSNLPYCPRVAYAMPLAPPpDgadtyTGDTLPQELQDPLKSYLTNFTISLG 317
CAR26813     340 ---nlspDGGVLFSRQPFRTKs-SNNCSLVFGLDFCSGVAYSVPSSSIs-----dNKTMLAQTFDHYSQSLFQNFSKALQ 410
XP_002551528 324 e--vlsdSGGILFSKATFTTMa-DDSCSLIFGLDFCDGVAYSVPTAKSaGt----NKTVTSLIYDGIASSLYSNFSKALQ 396
Q6CNE5       339 k--eledSGGVLFSKQLFETMd-DSSCSLIFGLGFCDGVAYSVPSSSLlEy---nDKDELAAIYDSIAESYYSNFSLALQ 412 Kluyveromyces l...
Q758F9       334 ----ttgIAGTIFQKTVFSTMe-NDACSLIFGLDFCKGVAYSVPTSSLqNk----DKTLMAGAYDAIASSLYANFSLALQ 404 Eremothecium go...
XP_003645827 337 k--nttdTGGVIFERVIFKTMe-NNSCSLIFGLEFCNGVAYSVPTSSKtDa---nDKILMAGMYDDIAKSLYFNFSLALE 410
XP_001644330 326 ---aildIGGVLFRRIDFTTKp-DNTCSLIFGLNFCSGVAYSVPTSSKfPs----DKDAIAKMYDSIAESLYANFSNALQ 397
XP_003688713 315 gssdensSGGLLYGNFTFSTKp-DNTCSLVFGLNFCSGVAYSVPTPSElQg----NKSAIVQIYDSIAESLYTNFTKALQ 389
XP_004179180 351 s-nndnsITGVVFSRQLITTKv-KSTCSIIFNLDICSSVAYAAPTPLKyYv----NKTAMANDYDSLVRSLYENFTTALQ 424
EME85774     471 QVACNTTSDAQYSLARNCTECAAAYKEWLCAVSIPRCEDFSNQ---APYLQPRNVGqkfynndsmldsdfllsnyvpmtk 547
EAU91143     318 RFACGRD---LYSPLVDCIDCQREYRKWLCAISLPRCSEPSPSnpnAFTALAPDPTatglsatkneqqqv---------- 384
CAR26813     411 IVPCEMELDARYSPVRTCQDCSDSYRDWLCAVTIPRCTLDNQE-----YYIRRKKDd----------------------- 462
XP_002551528 397 MIPCDTEKDARFSPLRTCNDCANSYRNWLCAVTIPRCTTENSS-----YYIHRDKTq----------------------- 448
Q6CNE5       413 LIPCDTEQDAVYSPLRKCSNCAASYKNWLCSVSIPRCTTDKTK-----YYVHRSAEe----------------------- 464 Kluyveromyces l...
Q758F9       405 LIPCDESTESRYSPLRHCEDCEQSYKSWLCAVTIPRCTSRPKE-----NYVARDSDd----------------------- 456 Eremothecium go...
XP_003645827 411 LIPCHSDADSIYSPLRRCEDCASSYKNWLCAVSIPRCTTDESD-----YYVFRKSSd----------------------- 462
XP_001644330 398 IIPCDTELDARYSPFRTCDDCAASYMNWLCAVSIPRCTTDESP-----LFIRRQKSq----------------------- 449
XP_003688713 390 IIPCDTELDARYSPLRTCDDCAASYLDWLCAVSIPRCTTTETP-----HFIYRNKNv----------------------- 441
XP_004179180 425 LIPCDAELDQRYSPLRTCEDCAQSYLNWLCSIMIPRCSSPGNF-----GSIFRKKFk----------------------- 476
EME85774     548 aptlegsiayqqtynwsfatnrsRNQMIDEVIRP-GPYKEVLPCEDLCYSLMQSCPASIQFACPYPGR---GLEVSYGKR 623
EAU91143     385 ----------lsalvplqtstppRNSNLPPFDSP---YQMLLPCLETCTAVDRACPPFLGFKCPTSQF---NGAASYGIG 448
CAR26813     463 ----------------------nRNQFINEEIKPmSDYYEVPPCIEMCYHMVRDCPSDFGFACPDINNqkdLFHRSYSYF 520
XP_002551528 449 ----------------------nRNDFLNQQIQPiHDYYEILPCIDMCYNLAADCPPDFQFSCPSKTShseLLYLSYSVW 506
Q6CNE5       465 ----------------------sRNEMLNELIKPsSDYYEILPCVDTCVAMLRDCPPDFGFTCPSYTSytdLMLNSYNFY 522 Kluyveromyces l...
Q758F9       457 ----------------------nRNALINDKIKPvSNYYEILPCIDMCHQMVRDCPSSFGFSCPSRSKfpqLAHRSYQSY 514 Eremothecium go...
XP_003645827 463 ----------------------nRNDLINDSIEPtSDYYEILPCINMCYAMVRDCPSEFNFACPNKSKypnLLYSSYREY 520
XP_001644330 450 ----------------------nRNSYINNDIAPiNDYYEVLPCIDMCYSMVRDCPSDFGFACPSITSnydLLSLSYNFL 507
XP_003688713 442 ----------------------nRNSYINEDIKPiRDYYEVLPCIDMCHSIVRDCPADFGFACPEPSNsnnLLFSSYNYY 499
XP_004179180 477 ----------------------sRNSLIDTFIQPtDNYYELMPCIDSCYEMVRDCPAQFGFSCPNIKSsdeLFRHSYSYF 534
EME85774     624 STS---G-LTCSYLGA 635
EAU91143     449 YIDgkeGdEKEGLTGQ 464
CAR26813     521 DTNsm--eDTCNYIGN 534
XP_002551528 507 EENd---fHTCNYVGN 519
Q6CNE5       523 ETDqs--yLTCNFVGN 536 Kluyveromyces lactis
Q758F9       515 SNVse--aLNCNYIGN 528 Eremothecium gossypii
XP_003645827 521 IEsa---vIPCNFVGN 533
XP_001644330 508 LENfd--fPTCNYIGD 521
XP_003688713 500 YEDfd--yITCNFIGN 513
XP_004179180 535 NSSes--sETCNFLGN 548
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