Conserved Protein Domain Family
CYP734

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cd20639: CYP734 
cytochrome P450 family 734
Cytochrome P450 family 734 (CYP734) belongs to the plant CYP72 clan, which is generally associated with the metabolism of a diversity of fairly hydrophobic compounds including fatty acids and isoprenoids, with the catabolism of hormones (brassinosteroids and gibberellin, GA) and with the biosynthesis of cytokinins. CYP734As function as multisubstrate and multifunctional enzymes in brassinosteroid (BRs) catabolism and regulation of BRs homeostasis. Arabidopsis thaliana CYP734A1/BAS1 (formerly CYP72B1) inactivates bioactive brassinosteroids such as castasterone (CS) and brassinolide (BL) by C-26 hydroxylation. Rice CYP734As can catalyze C-22 hydroxylation as well as second and third oxidations to produce aldehyde and carboxylate groups at C-26. CYP734 belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410732
Aligned: 9 rows
Threshold Bit Score: 724.625
Created: 31-Jul-2019
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
heme bindingputative
Feature 1:heme binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                            #   #    
O48786     85 LSFYHHWRKIyGATFLVWFGptFRLTVADPDLIREIFSKSEFyek----neaHPLVKQLEGDGLLSLKGEKWAHHRKIIS 160 thale cress
NP_177649  79 APHYHEWSRVyGKTFLYWFGskPVVATSDPRLIREALTTGGSfdr----ighNPLSKLLYAQGLPGLRGDQWAFHRRIAK 154 thale cress
ACF85368   64 TPQYAVWLARyGRPFVYWFGprPRLVISDPELVKAVMTDSTGgfdkaasggnNPLARQLIGEGLVGLSGETWARHRRVIS 143 maize
ABK25130   80 LPYYHHWTKTyGKDFIYWVGskPRLNVPHPELIKEILSNKFGhyen---issNPLGRQLVGQGLVGLRGEKWVQHRRIIN 156 Sitka spruce
B9X287     82 LAFYHHWKKIyGSTFLIWFGptPRLAIADPELIREVLLARADrfdr---yesHPMVRQLEGEGLVSLRGDKWAHHRRVLT 158 Japanese rice
Q69XM6     81 LAFYHYWRKIyGPTFLIWFGptPRLTVAEPEMVREIFLTRAEafdr---yeaHPVVRQLEGDGLVSLHGDKWAHHRRVLT 157 Japanese rice
Q6Z6D6     97 LAFYHYWRKIyGHRFLIWFGptPRLTVAEPELIREIFLTRADafdr---yeaHPVVRQLEGDGLVSLHGDKWALHRRVLT 173 Japanese rice
AAQ65187   85 LSFYHHWRKIyGATFLVWFGptFRLTVADPDLIREIFSKSEFyek----neaHPLVKQLEGDGLLSLKGEKWAHHRKIIS 160 thale cress
Q8LIF2     95 LPFYHHWRKLyGPMHLIWFGrtPRLVVSEPELIREVLLTRADhfdr---yeaHPMICQFEGYGLSNLHGERWARRRRVLT 171 Japanese rice
Feature 1       #                                                  #                          
O48786    161 PTFhmenlkllVPVVLKSVTDMVDKWSDKlsengev--evdvyeWFQILTEDVISRTAFGs-sYEDGRAVFRLQAQQMLL 237 thale cress
NP_177649 155 QAFtmeklkrwVPQMVTSTMMLMEKWEDMrnggeei--elevhkEMHNLSAEMLSRTAFGn-sVEEGKGIFELQERMMRL 231 thale cress
ACF85368  144 PAFnmervkswIPEIAAAASSVLDKWEAEggsrtef--eidvhkAFHTLSADVISCVAFGs-sYEEGKRIFQLQEEQMQL 220 maize
ABK25130  157 PAFhldflkgmVPTIVESTANMLEKWGKLvlsgae---evevlkEFCNLTADVISRTALGs-sYAEAKHIFNLQDQQMLL 232 Sitka spruce
B9X287    159 PAFhmdnlrllLPCVGMTVLDMADKWRAMaeadksgeveidvsdWFQVVTEDAITRTAFGr-sYEDGKVVFKLQAQLMAF 237 Japanese rice
Q69XM6    158 PGFypdnlnrlVPHVGRSVAALAERWRAMacaggge-vevdvaeWFQAVAEEAITRATFGr-sYDSGRVVFRLQARLMAF 235 Japanese rice
Q6Z6D6    174 DAFypdnlnrlIPHVGKSVAALAAKWGAMaeaggsgevevdvaeWFQAVTEEAITRATFGr-sYDDGRVVFAMQGQLMAF 252 Japanese rice
AAQ65187  161 PTFhmenlkllVPVVLKSVTDMVDKWSDKlsengev--evdvyeWFQILTEDVISRTAFGs-sYEDGRAVFRLQAQQMLL 237 thale cress
Q8LIF2    172 PAFhtenlrmiAPFVAGTVTRMLDELAERaraggageaevdvaeWFQRVPQEAITFAAFGrrnYDDGAAVFRLQDELAGY 251 Japanese rice
Feature 1                                                                                     
O48786    238 caeafqkvfiPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRrqnaidgeg------eeckepaakDLLGLMIQAKnv-- 309 thale cress
NP_177649 232 fylvrwsvyiPGFRFFPSKTNREIWRIEKQIRVSILKLIENNktav----------------eksgTLLQAFMSPYtnqn 295 thale cress
ACF85368  221 allamrtvyiPGFRFVPTKKNRKRQRLNQEIQCSLRKLIEINgtkc----------------edskNLLGLMLSASkags 284 maize
ABK25130  233 taelvlsvyvPGFRFLPTRKNRQRWKLEKEIRKCMRQVIEARertadie----------qsgsygtDLLGLMMSAKnkrv 302 Sitka spruce
B9X287    238 aseafrkvfiPGYRFLPTKKNTSSWKLDKEIRKNLVTLIGRRqeagdde----------kldgcakDLLGLMINAAassn 307 Japanese rice
Q69XM6    236 aseafrkvlvPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRsggdggeedetttelkdkqdsgfnDLLGLMINAGvdr- 314 Japanese rice
Q6Z6D6    253 aseafrkvlvPGYRFLPTKKNRLSWRLDREIRRSLMRLIGRRsdeaeqge--------kaddgsfrDLLGLMINAGaaaa 324 Japanese rice
AAQ65187  238 caeafqkvfiPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRrqnaidgeg------eeckepaakDLLGLMIQAKnv-- 309 thale cress
Q8LIF2    252 ateahskvyiPGYRFLPTRKNRRVWQLDREIRSHLAKFVTGLqscssshg---------ddaddggDGGGGMREFMsfma 322 Japanese rice
Feature 1                               ##  ##  ##  #                                         
O48786    310 ----------------tVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGsrdvpt--kdhvvk 371 thale cress
NP_177649 296 gq----------eeklgIEEVTDECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVICVLGqtglpt--ldilqd 363 thale cress
ACF85368  285 e------------ykmgIEEIIHECKTFYFAGKETTANLLTWATLLLALHQEWQVKARDEVLKVCGkhehpn--aenlsd 350 maize
ABK25130  303 ggk-------lqdvrmtTEEIIDECKTFYFAGHETTSILLTWTIILLGMHQDWQDRGRKEVLEVCGknvvpd--adsvnh 373 Sitka spruce
B9X287    308 ggkrs----alpvspitVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCGadgvps--reqlak 381 Japanese rice
Q69XM6    315 --------------tmpVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQDRARREVLAVCGdaagelptkdhlpk 380 Japanese rice
Q6Z6D6    325 trgnaggeknspaaaipVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDVCGagelps--kehlpk 402 Japanese rice
AAQ65187  310 ----------------tVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGsrdvpt--kdhvvk 371 thale cress
Q8LIF2    323 -------------pamtAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVCGrgdlpt--kdhlpk 387 Japanese rice
Feature 1                #        # #                              #                          
O48786    372 LKTLSMILNESLRLYPPiVATIRRAKSDVKLGg--------YKIPCGTELLIPIIAVHHDqaiWGNDVNEFNPARFADGv 443 thale cress
NP_177649 364 LKTLSMIINETLRLYPPaMTLNRDTLKRAKLGd--------LDIPAGTQLYLSVVAMHHDketWGDDAEEFNPRRFEDPk 435 thale cress
ACF85368  351 LKIVTMVLKETLRLYPPaTFINRTATRDIKLGk--------LDIPAGTRLDFPIIHIHRDhevWGMDAEEFNPSRFADGs 422 maize
ABK25130  374 LKIVGMILNEALRLYPPaVFLQRQAVKPMQLGr--------LSIPAGTQLLLPILAIHHDqclWGNDANEFNPARFSEGi 445 Sitka spruce
B9X287    382 LKTLGMILNETLRLYPPaVATVRRAKADVELGg-------yLRIPRDTELLIPIMAVHHDarlWGPDAAQFNPARFAGGv 454 Japanese rice
Q69XM6    381 LKTLGMILNETLRLYPPaVATIRRAKFDVTLGgggdgdaggIHIPRDTELLVPIMAIHHDarlWGPDAAQFNPARFASGa 460 Japanese rice
Q6Z6D6    403 LKTLGMIMNETLRLYPPaVATIRRAKVDVQLSd-------gCMIPRDMELLVPIMAIHHDtryWGPDASQFNPARFANGa 475 Japanese rice
AAQ65187  372 LKTLSMILNESLRLYPPiVATIRRAKSDVKLGg--------YKIPCGTELLIPIIAVHHDqaiWGNDVNEFNPARFADGv 443 thale cress
Q8LIF2    388 LKTLGMILNETLRLYPPaVAMIRTAKEDVELGg--------CVVPAGTEVMIPIMAVHHDaaaWGDDAAEFNPARFAADd 459 Japanese rice
Feature 1                  ###   #####  ##                                        
O48786    444 pr-aakHPVGF-IPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTfhlAPTYQHAPTvlmLLYPQHGAP 509 thale cress
NP_177649 436 ------KQSALlVPFGLGPRTCVGQNLAVNEAKTVLATILKYYSfrlSPSYAHAPVlfvTLQPQNGAH 497 thale cress
ACF85368  423 s----yHLGAY-FPFGIGPTICVGQNLAMVEAKVALAMTLQRFAftvSASYAHAPMlvfTLQPQFGAQ 485 maize
ABK25130  446 ak-avkHPLAF-MPFGFGPRICVGQNFALLEAKVVLAMILQRFSfvtSPSYAHAPVmvvTVRPQHGAQ 511 Sitka spruce
B9X287    455 ar-aarHPAAF-IPFGLGARMCIGQNLAILEAKLTVAVILHRFEfrlSARYVHAPTvlmLLHPQYGAP 520 Japanese rice
Q69XM6    461 ar-aakHPLAF-IPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDlalSPTYVHAPTvlmLLHPQYGAP 526 Japanese rice
Q6Z6D6    476 sk-aakHPLAF-IPFGLGSRMCVGQNLARLEAKLTMAILLQRFEirtSPNYVHAPTvlmLLYPQYGAP 541 Japanese rice
AAQ65187  444 pr-aakHPVGF-IPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTfhlAPTYQHAPTvlmLLYPQHGAP 509 thale cress
Q8LIF2    460 dggrrrHPMAF-MPFGGGARVCIGQNMALMEAKVALAVVLRRFEfrlSPAYVHAPRvlmILSPQFGAP 526 Japanese rice

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