3PUF,3P5J


Conserved Protein Domain Family
RNase_H2_suC
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pfam08615: RNase_H2_suC 
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Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
This entry represents the non-catalytic subunit of RNase H2, which in S. cerevisiae is Ylr154p/Rnh203p. Whereas bacterial and archaeal RNases H2 are active as single polypeptides, the Saccharomyces cerevisiae homolog, Rnh2Ap, when expressed in Escherichia coli, fails to produce an active RNase H2. For RNase H2 activity three proteins are required [Rnh2Ap (Rnh201p), Ydr279p (Rnh202p) and Ylr154p (Rnh203p)]. Deletion of any one of the proteins or mutations in the catalytic site in Rnh2A leads to loss of RNase H2 activity. RNase H2 ia an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication.
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Statistics
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PSSM-Id: 400782
Aligned: 19 rows
Threshold Bit Score: 88.1923
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
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Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
3PUF_L        31 TLHLLPCEVAVDGPAP-VGRFFTP-------------------------------------AIRQGPEG----------- 61  human
EDK40448      14 TVSLLPLHIQYSGPAN-TKEYFSDtk----------------------------------sTEKVGNEEr---------- 48 
CDS46262     114 NTNIFPFHIKKNGKIN-SDIFFIPyisednkdvitkytynydyydiypqnveltnknfhanKRKDIDSSrhgnlvkckke 192
Q8I300       109 DTNIFSFHIKKNGKIN-SDIFFIPynsiddedviteytysynyydiypvnieqvnksfhsnKKRKIIKNknintnnqlli 187 Plasmodium falc...
Q6CE25        43 VCHVLPCHIHYTGPAN-VAEYFTIeggdnkngqqvevtkde----kadenpevakkaeendAAKEQGEEdekefglnnrd 117 Yarrowia lipoly...
XP_002881655  91 qVHQLPCCIRFDGPAD-VSHYFKPqssvrlm-----------------------wravefvDVEIDGVRt---------- 136
XP_001646912  16 LGHLVPCKIRYNGPSDeFTDNFNMdneem---------------------------evlnnDVADESKQv---------- 58 
EDO06576       6 NVHVLPCLIKQPGDADiQGGFTDNiisldsvspe-----------------tplgglsisdICGSVSRDvnfgvsgterl 68 
Q4D5R7        14 VVHSLPFKTTFRGTTDiEENFSRH-------------------------------------IEHTENGG----------- 45  Trypanosoma cruzi
Q4GYA5        23 VIHSLPVKTNFRGTTDvTVNFTQH-------------------------------------TVRGEGGE----------- 54  Trypanosoma brucei

3PUF_L        62 -------------------------------LEVSFRGRCLRGEEVAVPP-GLVGYVMVTEEKKVsmgkp---dplrDSG 106 human
EDK40448      49 -------------------------------EIAYFRGCRLVGHKTNWDG-KYCGYIMNKSETIAh----------aNPA 86 
CDS46262     193 hid---------------kdeneneksnfqkFLVHFRGRLFIGSNINYSFfNIQTFLATLSN----------------DD 241
Q8I300       188 kkeldednndinneknenyndiknkrsnfqkLLVHFRGRLFIGCHINYSYfKIKTFLAMIKN---------------NED 252 Plasmodium falc...
Q6CE25       118 s------------------kdpkdksassapLSTHFRGRKLHGVEQNLPE-TYRGYRFTEGTNFFensnpvsqlgdiTQD 178 Yarrowia lipoly...
XP_002881655 137 -------------------------------EEAHFRGRKLQGATISLPS-GYSGFVLGQASNLN------------ANG 172
XP_001646912  59 -------------------------------YVNYIRGRKIIGQDV-LPIfGEGSKAFLTENVIS------------SDA 94 
EDO06576      69 scdv------------yssqygsdpflgkerLCTFLRGRCLRGSKLNFDAiGCRMSMVEVCNSTRq-----------RNA 125
Q4D5R7        46 -------------------------------LRNRLRGRTLIGREVVLPP-SYVLAVTSFMPRQQhplg-----tgaAGC 88  Trypanosoma cruzi
Q4GYA5        55 -------------------------------LRNALRGRTLMGREVILPS-SYVIACASFTNKPPiaeaggrksggaAAC 102 Trypanosoma brucei

3PUF_L       107 TDDQEEEPLERDFDRF-------IGATANFSRFTLWGLETIP--GPDAKVRGAlTWPSLAAAI 160 human
EDK40448      87 DTEGIANGTAEIKNVKv------YNPIAKFDSMTLYGHDNVI--ESTSQWRMVsEWSEISDAI 141
CDS46262     242 HTTSETQNKLCYVNKK-------IKTYNHIPNATYWKQDEYPd-IFDSNIQKL-QMFFISQSI 295
Q8I300       253 IDDTENLPLFNYVNKN-------IQTYNLIENATYWKQDEYPd-YTDQNIQKL-FFFHI---L 303 Plasmodium falciparum 3D7
Q6CE25       179 FDQDGFERNPDDYRNNepiemrsWNAEGSFDHLVIWGHERVPdlASDQWACGVrDWHQVATAM 241 Yarrowia lipolytica
XP_002881655 173 KRKASMPAEEN---QC-------WEVKAKFDNLTYWNHDSLPs-KDDTFLRSF-HWFSIAEAI 223
XP_001646912  95 TMEEEFDNTESIN----------AKPIATLNKLINYERDGNEs-RLSSELQKFqEYIELNDII 146
EDO06576     126 SSRSGLTPSYT------------LGELGPVESFTVWDRDSDIs-PLSPCLEGY-KHIAVARAL 174
Q4D5R7        89 VTSSAPSLAQVEAVASd------VRITAIEDRFCVWEHDKQP-----ERVDALaHWLELSRTL 140 Trypanosoma cruzi
Q4GYA5       103 FDTTQPSSAQVEAAAGd------MRITAVAESFCIWEHDKKP-----ERSEMVtQWLRLSNDL 154 Trypanosoma brucei
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