Conserved Protein Domain Family
TRM1

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TIGR00308: TRM1 
tRNA(guanine-26,N2-N2) methyltransferase
This enzyme is responsible for two methylations of a characteristic guanine of most tRNA molecules. The activity has been demonstrated for eukaryotic and archaeal proteins, which are active when expressed in E. coli, a species that lacks this enzyme. At least one Eubacterium, Aquifex aeolicus, has an ortholog, as do all completed archaeal genomes. [Protein synthesis, tRNA and rRNA base modification]
Statistics
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PSSM-Id: 273006
Aligned: 4 rows
Threshold Bit Score: 528.643
Created: 7-Oct-2014
Updated: 25-Oct-2021
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
O27258   4 VNEGSVTIRVPdfskvsARAPVFYNPAMEFNRDVSVVALQVFQRLLGGE------------------------------- 52  Methanothermobacter t...
Q58356   3 LKEGEVVFEVPdkltvtKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRd------------------------------ 52  Methanocaldococcus ja...
O29443   3 VEEGRARVKV---------EGVFYNPRMRFCRDLDML---VFATMDSK-------------------------------- 38  Archaeoglobus fulgidu...
P15565  45 VKEGKAEILFP------KKETVFYNPIQQFNRDLSVTCIKAWDNLYGEEcgqkrnnkkskkkrcaetnddsskrqkmgng 118 Saccharomyces cerevis...
O27258  53 ---------------ISVADTFSGSGIRAIRYLVEVE--GVSEAFANDINPLAVECIKNNSVINSVS-PEVSREDASIFL 114 Methanothermobacter t...
Q58356  53 --------------kFYIADALAGSGIRGLRYAKELEfnGELKVFLNDINPKAYEKIINNAKLNEIEnIDVFNEDANTFL 118 Methanocaldococcus ja...
O29443  39 ----------------EYFDALSASGIRGIRAALEAG----KKAVFNDVSPKAVKVIEENLRENGVS-GEVINGDAAAVM 97  Archaeoglobus fulgidu...
P15565 119 spkeavgnsnrnepyINILEALSATGLRAIRYAHEIP--HVREVIANDLLPEAVESIKRNVEYNSVEnIVKPNLDDANVL 196 Saccharomyces cerevis...
O27258 115 RSN----HGRFDVIDIDPFGTPAPFMDSAA-ASARNnSLLAVTATDTSSLCGT-YIKPCLRKYS-SRPLKTEYCHETGLR 187 Methanothermobacter t...
Q58356 119 SKH----FRFFNVVDIDPFGSPAPYVEQAIrALVTRnGLLCLTATDTAALCGR-SKKSCLRKYL-AYPLFGRDCHEFALR 192 Methanocaldococcus ja...
O29443  98 RQ------RAFEHIDIDPFGSPAPFMDSAC-FSAKR--YLSVTATDTAALCGS-ATNSGLKKYG-AFAVKTDVYHEVGLR 166 Archaeoglobus fulgidu...
P15565 197 MYRnkatNNKFHVIDLDPYGTVTPFVDAAIqSIEEG-GLMLVTCTDLSVLAGNgYPEKCFALYGgANMVSHESTHESALR 275 Saccharomyces cerevis...
O27258 188 ILAGFTAMNLARYRKAASVLLSHSSQHYMRLYIRVRRGARRADESIRNIGFMlhcfr--clhhEHVNGFAPLKRe----- 260 Methanothermobacter t...
Q58356 193 VLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEMLGYV-----------KDVNGIKIIKK------ 255 Methanocaldococcus ja...
O29443 167 MLIGFVVREATKYEKALFPLISWVREHYYRVHFKIKKSTAMSAKVYEKMGYLaycs---tclrKKVLGMGEGAEr----- 238 Archaeoglobus fulgidu...
P15565 276 LVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTMTTyhcsrcgsyhnQPLGRISQREGrnnktf 355 Saccharomyces cerevis...
O27258 261 --------------cPHCGAEMDVAGPLWVGDIQDSKFIGEMIGEVENKE------LNTAgdVLKLLKGCLDEAGMPP-G 319 Methanothermobacter t...
Q58356 256 ---------------FEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKRE------FSER--VLKILNAIKGESAINQvG 312 Methanocaldococcus ja...
O29443 239 ---------------CECGGKFSLIGPIWLGELKQRDFAEKVAEKAEGK-------------LRAFLEKILAEIDAPT-- 288 Archaeoglobus fulgidu...
P15565 356 tkysvaqgppvdtkcKFCEGTYHLAGPMYAGPLHNKEFIEEVLRINKEEHrdqddtYGTRkrIEGMLSLAKNELSDSP-F 434 Saccharomyces cerevis...
O27258 320 FYDIHEVCSKLGRSAPPLRDVMDGLEAAGFRVSRTHIRPTGIRTDAGIGEIEEVLAGLMPE 380 Methanothermobacter thermautotrophicus s...
Q58356 313 CYDTHQIGKMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATLKNVIEAIYQCTKI 373 Methanocaldococcus jannaschii DSM 2661
O29443 289 AYSLPALAKIHSLTLPPTDVVVEELKKLGYEASRTHYCGFCVKTDADRDVVVKILRSRKII 349 Archaeoglobus fulgidus DSM 4304
P15565 435 YFSPNHIASVIKLQVPPLKKVVAGLGSLGFECSLTHAQPSSLKTNAPWDAIWYVMQKCDDE 495 Saccharomyces cerevisiae S288c
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