1YLV,1QNV,1H7O,1E51,1PV8,1EB3


Conserved Protein Domain Family
eu_ALAD_PBGS_cysteine_rich

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cd04824: eu_ALAD_PBGS_cysteine_rich 
Click on image for an interactive view with Cn3D
Porphobilinogen synthase (PBGS), which is also called delta-aminolevulinic acid dehydratase (ALAD), catalyzes the condensation of two 5-aminolevulinic acid (ALA) molecules to form the pyrrole porphobilinogen (PBG), which is the second step in the biosynthesis of tetrapyrroles, such as heme, vitamin B12 and chlorophyll. This reaction involves the formation of a Schiff base link between the substrate and the enzyme. PBGSs are metalloenzymes, some of which have a second, allosteric metal binding site, beside the metal ion binding site in their active site. Although PBGS is a family of homologous enzymes, its metal ion utilization at catalytic site varies between zinc and magnesium and/or potassium. PBGS can be classified into two groups based on differences in their active site metal binding site. The eukaryotic PBGSs represented by this model, which contain a cysteine-rich zinc binding motif (DXCXCX(Y/F)X3G(H/Q)CG), require zinc for their activity, they do not contain an additional allosteric metal binding site and do not bind magnesium.
Statistics
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PSSM-Id: 240128
Aligned: 16 rows
Threshold Bit Score: 568.919
Created: 20-Feb-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1H7O_A, Saccharomyces cerevisiae ALAD_PBGS; Complex of active site with 5-aminolaevulinic acid
  • Comment:The active site of ALAD contains A-site and P-site, each binding one molecule of 5-aminolevulinic acid (ALA); the A-site contains the metal ion binding site

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1YLV_A        19 AGGYNHPLLRQWQs-eRQLTKNMLIFPLFISDnpdDFTEIDSLPNINRIGVnRLKDYLKPLVAKGLRSVILFGVPlipgt 97  baker's yeast
1QNV_A        19 AGGYNHPLLRQWQs-eRQLTKNMLIFPLFISDnpdDFTEIDSLPNINRIGVnRLKDYLKPLVAKGLRSVILFGVPlipgt 97  baker's yeast
1H7O_A        19 AGGYNHPLLRQWQs-eRQLTKNMLIFPLFISDnpdDFTEIDSLPNINRIGVnRLKDYLKPLVAKGLRSVILFGVPlipgt 97  Saccharomyces c...
1EB3_A        19 AGGYNHPLLRQWQs-eRQLTKNMLIFPLFISDnpdDFTEIDSLPNINRIGVnRLKDYLKPLVAKGLRSVILFGVPlipgt 97  baker's yeast
XP_001032351  15 HGGYKHPACRNWVk--QTLSANNLVYPVFVLDepdAEQEIKTMPGIKRFGYkKIVDHLKPLVKKGLQSIIIFGILsddnk 92  Tetrahymena the...
XP_782371     14 HSTFHHEVLRSWQnidTTITPDNLVYPLFIIDnpdLIEPVSSLPGVNRYGVnTLQAALTPLVAKGLKAVLLFGVPtglpk 93  purple urchin
XP_758207     21 HSGYQHPVTRAWQnqgRNITKSMFMYPIFVTDdadAEVPLASMPGQCRWGVnKLEGFLAPLVKKGLSSVILFGVPlktpk 100 Ustilago maydis...
CAD70821      22 HGGYAHPLARSWQa-eRQLTKSMLIYPLFVSDnddEEVLIPSLPNQYRRGInRLVPFLEPLVHKGLRSVILFGVPlkpgt 100 Neurospora crassa
CAK74379       5 HPLFAHPTQKLWNs--CLPRKSQLVYPIFVVDgenQKQPINSMPNINRYSVdLLKEFLEPLVIKGLKAVLIFGVIgnedk 82  Paramecium tetr...
NP_648564      6 HSGMHHATLRQLQesgCEIAPHNLMYPVFIVSnddDVQPIASMPGISRFGLnRLKEHLEPLVAKGLSSVLLFGVVdpdmk 85  fruit fly
Feature 1                                           # # #       #                                
1YLV_A        98 kdp--vgtaaddpAGPVIQGIKFIREKFpeLYIICDVCLCEYTSHGHCGVLYddGTINRERSVSRLAAVAVNYAKAGAHC 175 baker's yeast
1QNV_A        98 kdp--vgtaaddpAGPVIQGIKFIREYFpeLYIICDVCLCEYTSHGHCGVLYddGTINRERSVSRLAAVAVNYAKAGAHC 175 baker's yeast
1H7O_A        98 kdp--vgtaaddpAGPVIQGIKFIREYFpeLYIICDVCLCEYTSHGHCGVLYddGTINRERSVSRLAAVAVNYAKAGAHC 175 Saccharomyces c...
1EB3_A        98 kdp--vgtaaddpAGPVIQGIKFIREYFpeLYIICDVCLCEYTSHGHCGVLYddGTINRERSVSRLAAVAVNYAKAGAHC 175 baker's yeast
XP_001032351  93 kdergshaggngiKGPTNLALEEIKKEIpeLLLIVDVCLCAFTSHGHCGLLNceGYIDNAASLKRLVEVSISYVQSGAQV 172 Tetrahymena the...
XP_782371     94 dtr---gssadteDSPVIQAIHVISEKFpqLLISCDVCLCAYTSHGHCGILNegGTLDNEASIQRLAEVALSYAQAGCHV 170 purple urchin
XP_758207    101 daq---gtladdpNGPVMQAIKALRKAFpsLYIATDVCLCEYTSHGHCGILYedGTINNQPSVERLAEVAVNYAKAGAHC 177 Ustilago maydis...
CAD70821     101 kda--lgtaaddpEGPVIRSIRLLRQRFpqLYITVDVCLCEYTSHGHCGILRddGSLNNQLSVDRISDVALAYAKAGAHC 178 Neurospora crassa
CAK74379      83 ddvg-smagglgkDSCVHKALRVLKQAFpqLLLITDVCLCAYTSHGHCGILTkdGYLDNQASIKRLSECALSYAQAGADI 161 Paramecium tetr...
NP_648564     86 deq---asnadsaKNPVVLALPKLREWFpdLLIACDVCICPYSSHGHCGLLGe-TGLENGPSIKRIAEIAVAYAKAGAHI 161 fruit fly
Feature 1           #                              #     #  ##    #      #   #                   
1YLV_A       176 VAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSAPsNGDRKCYQLPPAgRGLARRALERDMSE 255 baker's yeast
1QNV_A       176 VAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSAPsNGDRKCYQLPPAgRGLARRALERDMSE 255 baker's yeast
1H7O_A       176 VAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSAPsNGDRKCYQLPPAgRGLARRALERDMSE 255 Saccharomyces c...
1EB3_A       176 VAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSAPsNGDRKCYQLPPAgRGLARRALERDMSE 255 baker's yeast
XP_001032351 173 IAPSDMMDGRIGAIKQELIRQNl--EIPVMSYAAKFASSYYGPFRDAckSAPgKSDRQAYQLPLDsTQLALKAVQRDLEE 250 Tetrahymena the...
XP_782371    171 VAPSDMMDGRVGAIKHLLMTNGlgnKVSVMSYSAKFASCFYGPFRDAakSAPsFGDRRCYQLPPGaRGLAMRAVDRDVAE 250 purple urchin
XP_758207    178 VAPSDMMDGRVRAIKKGLIDAGfgnKVTLMAYSAKFASSLYGPFRDAvdSAPsFGDRRCYQLPPTaRGLARRAIVRDASE 257 Ustilago maydis...
CAD70821     179 VAPSDMNDGRIRAIKLKLIEEGivhRVTLMSYAAKFSGCLYGPFRDAagSCPsFGDRKCYQLPPGgRGLARRAIVRDINE 258 Neurospora crassa
CAK74379     162 VAPSDMMDNRVRAIKELLDQHPn-lRVPIMSYSSKFCSALYGPFRDVccSAPqKFDRSSYQLPPGaRQLALQAAERDLKE 240 Paramecium tetr...
NP_648564    162 VAPSDMMDNRVKAIKQALIDAQm-nSVSLLAYSAKFTSNFYGPFREAaqSAPkFGDRRCYQLPSGsRSLAMRAIQRDVAE 240 fruit fly
Feature 1               #                       #  #                                      #      
1YLV_A       256 GADGIIVKPSTFYLDIMRDASEIckdLPICAYHVSGEYAMLHAAAEKGvvdLKTIAFESHTGFLRAGARLIITYLAPEFL 335 baker's yeast
1QNV_A       256 GADGIIVKPSTFYLDIMRDASEIckdLPICAYHVSGEYAMLHAAAEKGvvdLKTIAFESHQGFLRAGARLIITYLAPEFL 335 baker's yeast
1H7O_A       256 GADGIIVKPSTFYLDIMRDASEIckdLPICAYHVSGEYAMLHAAAEKGvvdLKTIAFESHQGFLRAGARLIITYLAPEFL 335 Saccharomyces c...
1EB3_A       256 GADGIIVKPSTFYLDIMRDASEIckdLPICAYHVSGEYAMLHAAAEKGvvdLKTIAFESHQGFLRAGARLIITYLAPEFL 335 baker's yeast
XP_001032351 251 GADYIMVKPITTYLDIVKEIKQRf-nPIMACYHVSGEYAMVCFAAQNGacdKKKVVLETMRSFRRAGIDIIITYFTPELL 329 Tetrahymena the...
XP_782371    251 GADYLMVKPGMPYLDIVRDTKEKhpdLKLAVYHVSGEYAMLYHGANQGafdLSKAVMEAVKCMRRAGADIIITYFVPDIL 330 purple urchin
XP_758207    258 GADVLMVKPTLPYLDIVAEARDLapdHPLACYQVSGEFAMIHAGAKAGvyeLRRMAEESVQSMVRAGANIILSYFTPEFL 337 Ustilago maydis...
CAD70821     259 GADIIMVKPASQYLDIISDAKELgkdLPVAAYQVSGEFAMIHAAAKAGvfdLKAMAFEATEGILRAGATIIISYFVPEFL 338 Neurospora crassa
CAK74379     241 GAGYVMVKPITAYLDIAAEIKRRhpdTLLACYHVSGEYSMIHAGAEKKlfdLKRVVLEYMEGFVRSGIDIIITYFTPELL 320 Paramecium tetr...
NP_648564    241 GADMLMVKPGMPYLDILRSTKDSypyHTLYVYQVSGEFAMLYHAAKAGafdLKDAVLEAMKGFRRAGADCIITYYTPFLL 320 fruit fly
Feature 1           
1YLV_A       336 DWL 338 baker's yeast
1QNV_A       336 DWL 338 baker's yeast
1H7O_A       336 DWL 338 Saccharomyces cerevisiae
1EB3_A       336 DWL 338 baker's yeast
XP_001032351 330 DWV 332 Tetrahymena thermophila SB210
XP_782371    331 EWI 333 purple urchin
XP_758207    338 EHK 340 Ustilago maydis 521
CAD70821     339 DWL 341 Neurospora crassa
CAK74379     321 DWL 323 Paramecium tetraurelia
NP_648564    321 DII 323 fruit fly

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