1DRT,1JR7


Conserved Protein Domain Family
CAS_like

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cd00250: CAS_like 
Click on image for an interactive view with Cn3D
Clavaminic acid synthetase (CAS) -like; CAS is a trifunctional Fe(II)/ 2-oxoglutarate (2OG) oxygenase carrying out three reactions in the biosynthesis of clavulanic acid, an inhibitor of class A serine beta-lactamases. In general, Fe(II)-2OG oxygenases catalyze a hydroxylation reaction, which leads to the incorporation of an oxygen atom from dioxygen into a hydroxyl group and conversion of 2OG to succinate and CO2
Statistics
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PSSM-Id: 238154
Aligned: 20 rows
Threshold Bit Score: 163.337
Created: 4-Sep-2001
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:iron coordination sites [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1DRT_A     28 LYAFLDAAHTAAASl----------------pGALATALDTFNAEgs-eDGHLLLRGLPVEadadlpttpsstpapedrs 90  Streptomyces clavu...
1JR7_A     46 KSFLRFRVAKILDDlc---------------aNQLQPLLLKTLLNr--aEGALLINAVGVDdvk---------------q 93  Escherichia coli
6523411   146 VSKLPDVLHFGYDQyfs------------qegFSKVLADVNRYGIs--fVSNIPDPALNIKtq----------------- 194 Pichia farinosa
9937216    51 RIRAELTGMRLYDSpr---------------aLVVTGFGVDDERIg--pTPAARPAPDPERt------------------ 95  Streptomyces verti...
8052402    62 RLRAAVRAFRRNSGec--------------gvTLVTGLPVDEALLp--pTPTADGSVRREAt------------------ 107 Streptomyces coeli...
7481144    59 LRRDLAGFRRDSGPd----------------gAIVLRGLPVDSMGl--pPTPRVNGSVQREa------------------ 102 Streptomyces coeli...
11349129   28 ETLFARLLERRPADs----------------aEALLRQRLHHELEh--gSGVLLHDSALLRgl----------------- 72  Pseudomonas aerugi...
3878668    88 IREGKVEKNVSNDNkiyelwn-----skslkdVPRISKSTLSLQSf--sKNLVKYGVIIVDg------------------ 142 Caenorhabditis ele...
6729662    56 VQRFLREFKLTDHEgh--------------avIRGHEFDQQRIGPt--pDDWRGRQRPGPEf------------------ 101 Streptomyces rochei
731603    127 GSSFVSPATRKQESryrpqlwnkrilkdnvkdLLSVSYNEFIDPKddskLFQTLVNLQKFGi------------------ 188 baker's yeast
Feature 1                                                                   # #               
1DRT_A     91 llTMEAMLGLVGRR---LGLHTGYr-eLRSG----TVYHDVYPspgah-hlsseTSETLLEFHTEMAYHRLQPNYVMLAC 161 Streptomyces clavu...
1JR7_A     94 adEMVKLATAVAHL---IGRSNFD---AMSG----QYYARFVVknvdnsdsylrQPHRVMELHNDGTYVEEITDYVLMMK 163 Escherichia coli
6523411   195 elSESNVGDWPIAK---LARKFGYikeTFYG----TLFDVKNKdeae----niaYTDTFLPLHMDLLYYESPPGLQFLHF 263 Pichia farinosa
9937216    96 -rDLELLLLLHAAL---LGEAFGWa-tQQNG----RLVHDVLPvpgeetaqmgsSSETELLWHTEDAFHPLRCDYVGLLC 166 Streptomyces verti...
8052402   108 -vPAATLLMTAAGG---LGDPAAYa-pEKSG----ALVQDVVPvpgke-dfegnAGSTLLSFHVENAFHVHRPDFVMLLC 177 Streptomyces coeli...
7481144   103 -sLGAAVLLMTACG---LGDPGAFl-pEKNG----ALVQDVVPvpgme-efqgnAGSTLLTFHNENAFHEHRPDFVMLLC 172 Streptomyces coeli...
11349129   73 -aEDEFQRVFRAFC---QWLGRTVa--INRN----GEYLKEVRdlglr--dardAPQRGHLTSQELAFHSDRADLTVLAC 140 Pseudomonas aerugi...
3878668   143 -vEGTSEATEKLCQ---SLVPVHDt-fFGQF----WVFSNSATndepa-yedtaYGSDEIGPHTDGTYFDQTPGIQVFHC 212 Caenorhabditis ele...
6729662   102 -pEELLLMLYAALL---GEPFGWAt--QQDG----HLVHDIFPirqhendqlgmGSKELLTWHTEDAFHPYRSDYLILGA 171 Streptomyces rochei
731603    189 -aFISGTPSSSSEGltiQKICERIg-pIRSTvhgeGTFDVNASqats---vnahYANKDLPLHTDLPFLENVPGFQILQS 263 baker's yeast
Feature 1                                                                                     
1DRT_A    162 SRADHe----rTAATLVAS-VRKAlpll------derTRARLLdRRMPCCVDVafrggv---------------ddpgai 215 Streptomyces clavu...
1JR7_A    164 IDEQNm----qGGNSLLLH-LDDWehl-------dnyFRHPLArRPMRFAAPPskn---------------------vsk 210 Escherichia coli
6523411   264 IKNSTe-----GGESMFADgFAIArkvke----qdpeAYDALKkVLITYRYENngh----------------------yy 312 Pichia farinosa
9937216   167 LRNHQr-----AATTVGWPdLSRLtt----------eDRAVLL-EPRYLIRPDtshtpaqnatgtrsaerfaaiaemdda 230 Streptomyces verti...
8052402   178 LRADHd-----GAAGLRTGsIRQAlpvl------serTREALFrPEFSTQPPPsfgt-------------------aagt 227 Streptomyces coeli...
7481144   173 LRADPt-----GRAGLRTAcVRRVlpll-----sdstVDALWA-PEFRTAPPPsfql-------------------sgpe 222 Streptomyces coeli...
11349129  141 WSPARr-----GGAFRIRSsADVLatlea----anpaWLPLLG-QPIPHDLRDegd----------------------ad 188 Pseudomonas aerugi...
3878668   213 LTPAKt-----GGDTVLVDsFYCAeklrn----espeDFEILCnTKISHHYLEgsppgs----------------sihsv 267 Caenorhabditis ele...
6729662   172 LRNPDrvpttlGGLDVASLsAEDIdil--------fePRFSIA-PDESHLPKNntitgeeeear------fatiqrmide 236 Streptomyces rochei
731603    264 LPATEg----eDPNTRPMNyFVDAfyatrnvresdfeAYEALQiVPVNYIYENgdk----------------------ry 317 baker's yeast
Feature 1                                                                                     
1DRT_A    216 AQVKPLYGDa---------------------------dDPFLGYDRe--LLAPEDp------------------ADKEAV 248 Streptomyces clavu...
1JR7_A    211 DVFHPVFDVdq-------------------------qgRPVMRYIDq--FVQPKDf------------------EEGVWL 245 Escherichia coli
6523411   313 YHKRPLVVEetsw----------------------etlDYASGIIKevnYSPPFQghfeygihgdkpkesalfkLFLRGY 370 Pichia farinosa
9937216   231 PERVAVLFGdp--------------------------eDPYLRIDPaymSPAPGDa------------------AARRAY 266 Streptomyces verti...
8052402   228 TPPRPVLTGdp--------------------------eDPDLRVDFs-aTTAVTP-------------------EARRAL 261 Streptomyces coeli...
7481144   223 EAPAPVLLGdr--------------------------sDPDLRVDLa--ATEPVTe------------------RAAEAL 256 Streptomyces coeli...
11349129  189 YALVPLLTEtp--------------------------rTFVLRYIRkfnESVTRHgls-------------lapQVRSML 229 Pseudomonas aerugi...
3878668   268 SLEKPVIERnsf------------------------gnITQIRFNPy--DRAPFSclnssea------saaetiKFYEAY 315 Caenorhabditis ele...
6729662   237 RPLGPLLYGsr--------------------------lDPYMRLDPy-fTSVPEGd-----------------tDARRAY 272 Streptomyces rochei
731603    318 YQSKPLIEHhdinedntllgnyealikcinysppyqapFTFGIYDKpsdLNNNLDlnlittpak--lterflfkSFIRGL 395 baker's yeast
Feature 1                                     #                        
1DRT_A    249 AALSKALDe--VTEAVYLEPGDLLIVDNFRTTHARTPFSprwdgkDRWLHRVYIRTD 303 Streptomyces clavuligerus
1JR7_A    246 SELSDAIEtskGILSVPVPVGKFLLINNLFWLHGRDRFTphp-dlRRELMRQRGYFA 301 Escherichia coli
6523411   371 LLFESLANqeqNQLSLKVPAGVCVIFDNRRILHSRKSFSssn-ggQRWLMGCYVDGD 426 Pichia farinosa
9937216   267 DTVTALIEd--ELRHVVLDAGSLLLVDNYQAVHGRKPFAaaydgrDRWLKRVNITRD 321 Streptomyces verticillus
8052402   262 VELEAAVDa--ASHTVRLTPGDLAIVDNRVTTHGRTAFRprydggDRWLLRTFAVAD 316 Streptomyces coelicolor A3(2)
7481144   257 RELQAHFDa--TAVTHRLLPGELAIVDNRVTVHGRTEFTprydgtDRWLQRTFVLTD 311 Streptomyces coelicolor
11349129  230 DGLDEAIEradGYAELDFSKGMLVLVNNHTTLHARTEFSdde-rqRRCLLRCWLSSE 285 Pseudomonas aeruginosa
3878668   316 EKFSKICHnpdNSIEISLRPGSVIFIDNFRILHSRTSFQg-----YRQMCGCYLSRD 367 Caenorhabditis elegans
6729662   273 DALYKLVDa--GMREVVADQGDVLFIDNHRAVHGRLPFKahydgtDRWLKRVCVTAD 327 Streptomyces rochei
731603    396 NLFESHINdfnNQFRLQLPENCCVIFNNRRILHANSLTSs----nQQWLKGCYFDSD 448 baker's yeast

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