Conserved Protein Domain Family
M14_Endopeptidase_I
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cd06229: M14_Endopeptidase_I 
Peptidase M14 carboxypeptidase family-like domain of Endopeptidase I
Peptidase M14-like domain of Gamma-D-glutamyl-L-diamino acid endopeptidase 1 (also known as Gamma-D-glutamyl-meso-diaminopimelate peptidase I, and Endopeptidase I (ENP1); EC 3.4.19.11). ENP1 is a member of the M14 family of metallocarboxypeptidases (MCPs), and is classified as belonging to subfamily C. However it has an exceptional type of activity of hydrolyzing the gamma-D-Glu-(L)meso-diaminopimelic acid (gamma-D-Glu-Dap) bond of L-Ala-gamma-D-Glu-(L)meso-diaminopimelic acid and L-Ala-gamma-D-Glu-(L)meso-diaminopimelic acid(L)-D-Ala peptides. ENP1 has a different substrate specificity and cellular role than MpaA (MpaA does not belong to this group). ENP1 hydrolyzes the gamma-D-Glu-Dap bond of MurNAc-tripeptide and MurNAc-tetrapeptide, as well as the amide bond of free tripeptide and tetrapeptide. ENP1 is active on spore cortex peptidoglycan, and is produced at stage IV of sporulation in forespore and spore integuments.
Links
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Statistics
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PSSM-Id: 349448
Aligned: 18 rows
Threshold Bit Score: 246.868
Created: 11-Mar-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Zn binding siteactive site
Feature 1: Zn binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:[H] [ED] [H]Click to see conserved feature residue pattern help
Evidence:
  • Comment:Metallocarboxypeptidases share the zinc binding motif HXXE...H, where the zinc ion is penta-coordinated to ND1 atoms of the histidines, OE1 and OE2 atoms of the glutamic acid, and to a water molecule in a slightly distorted tetrahedral manner.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1            #  #                                                                     
CAA49259  155 IHYKASFHANEWITTPIIMTFLNDYllaltnqttir-----------------------glsmgplynqTTLSLVPMVNP 211 Lysinibacillus sph...
EDO61364   62 VLLCGAFHGMEWLTSLLLLRFTSQMaaaletgslis-----------------------diklgdflrrRGVTVIPCVNP 118 Clostridium leptum...
CDB87905   55 ILLTAGHHANEYITSMLCWELVEAYlaavvqggsfg-----------------------grdagalyhnAMLYVVPMVNP 111 Firmicutes bacteri...
SCY38221   82 VHINGSFHARERITTNIILKNIEDYangyvnnskil-----------------------gydvkelldeVTIFFVPMVNP 138 Alkaliphilus pepti...
CDE72318   42 ILVVGGVHAREYISTFVCLDLFFDC--------------------------------------------DGVEILPMANP 77  Acidaminococcus sp...
OYP45385  132 IMVQATMHAREYMATQLTMALLEHYaqmcyqdicey----------------------kgqrisnllqqVCFVIIPMVNP 189 Prevotella sp. P4-98
CDB12524  199 VYVETSIHAREYMNTKFMMKVIEEYcrgydtkkyq------------------------gknystifnkVKLVIMPMVNP 254 Eubacterium sp. CA...
CDD35348   56 LVLTASIHGREYINTTLLLDMLAYYlprynhrelpd-----------------------apcyrelwrdVCIIFLPMANP 112 Roseburia sp. CAG:309
CRZ34285   56 ILFCAGVHGRETINPIVLLKIAEYYakiyeefnnatekfenlikkpdgdvqkqynrmifgkcvyellrtYTILIIPLLNP 135 thermophilic bacte...
SFI25618   29 ILITASIHAREYMTSQLVMRMLEEYvlnydvpnad------------------------gityrqrftnTCFYIIPMVNP 84  Pseudobutyrivibrio...

Feature 1                                                                                     
CAA49259  212 DGVNLVINgppanealknkl-------iawnhnsqnfsgWKANINGVDLNDQFPakwelena----rnpqtpgpRDYGGE 280 Lysinibacillus sph...
EDO61364  119 DGVEISLHgsaaageyrel---------vhnvscgdtsrWQANARGVDLNHNFNagwealhtlereqgiyhpapTRYGGE 189 Clostridium leptum...
CDB87905  112 DGIALVTGelspdsaeyraaqe----iaaaypdipfpagWKANLQGTDLNLNYPagwekartikaalgfsgpapRDYPGA 187 Firmicutes bacteri...
SCY38221  139 DGVDYTILgkesikspllreefdkivehpqstwftpglrWKSNIRGIDLNRQWDfgwdeesr----fdidvpadAHFKGF 214 Alkaliphilus pepti...
CDE72318   78 DGVRISDEgfsfidtvrdkisd----dvyslmkrsdlkmWKANLNAVDINVNFDarwgegsq-----nkryispSDYIGK 148 Acidaminococcus sp...
OYP45385  190 DGVEIAQNgsngqltdevkqw-----vrqtedngtshtkIKANANGVDINRNFGngfnnpfts---srkthrsfNFYSGL 261 Prevotella sp. P4-98
CDB12524  255 DGVTISQYgpkkirdinlrqn------lykiakgasfkeWKTNARGVDINRNFAegfgres-------akapgqKLYAGK 321 Eubacterium sp. CA...
CDD35348  113 DGVSISQNtsa--------------------------kkWKGNSAGVDLNRNFPcgfgl----------skdikKHNPGK 156 Roseburia sp. CAG:309
CRZ34285  136 DGYMISLYgynaiqdldlrk--------rvkkmginhteWKYNGRGVDINRNFPcr-----------------sWKPKGP 190 thermophilic bacte...
SFI25618   85 DGVTICQNgt---------------------------ksIKANANGVDLNRNFPlgfgsgpr-----laknpglAYYPGV 132 Pseudobutyrivibrio...

Feature 1                                  #                                                  
CAA49259  281 APl--TQPEAIAMADLTRsr-nFAWVLAFHTqGRVIYWGfenlep---pesqtMVEEFSRVs----GYEPIQsa------ 344 Lysinibacillus sph...
EDO61364  190 YPe--SEPETRLLCDFCRsq-yFRHALAFHSqGEEIYWDfgertp---eksrlMAQVLAASs----GYEMSEpea----- 254 Clostridium leptum...
CDB87905  188 RPl--DQPETAALAAYTCcv-rPELVLAYHTqGSVIYHTygglql---peadvLAEEFAAAs----GYSVEEvpa----- 252 Firmicutes bacteri...
SCY38221  215 EPh--SEAEVKAMEKLSIsn-pFLIYAAYHTqGLEIYWYkyqqged-leevkdITRKISNLt----FFRPVPalnnipqn 286 Alkaliphilus pepti...
CDE72318  149 MPf--SEPETRIIRSLLLd---KKTVICYHSkGEEIYYGfhnd------vgkeLALSLAKVt----GYEAKRad------ 207 Acidaminococcus sp...
OYP45385  262 LPy--SEPESQLMLRVSQry-dYDLFLNYHTsGNIIYYGcqnak----kyvnkLAHDYAKLihchtNYPLYGpds----- 329 Prevotella sp. P4-98
CDB12524  322 KAv--SEPETKAQIAVVKkv-kPNVVICYHQaGEVMYHRkn----------tkLSNMLYSMt----KYTHVIsep----- 379 Eubacterium sp. CA...
CDD35348  157 TAg--DQPETQHLMAFISqlpaPLGVIHYHSqGSLIYYDydvtg-----slrnHIVKIASIahattGYPLIEargknags 229 Roseburia sp. CAG:309
CRZ34285  191 RDyaaSENETQTLIQIFHqy-rIRCFLDFHSrGKSIYYYrnsmtssynenqykIAKKLKEIt----GYELVSpenei-ey 264 thermophilic bacte...
SFI25618  133 EPl--SEPETAAMAALLNen-vFCAGINYHSmGNIIYYGaatntpevaqgcrqMADLVHSIi----GYRREYcg------ 199 Pseudobutyrivibrio...

Feature 1                                                      
CAA49259  345 -nsYAGYKDWFIqdwrrPGFTVELGsgtnpl--pisefdTIYQEALGIF 390 Lysinibacillus sphaericus
EDO61364  255 iatGGGFKDWFIscfcrPAFTIEIGkgenpl--plsdlpGIHRTLEEML 301 Clostridium leptum DSM 753
CDB87905  253 esaNAGFKDWFLqrfrrPGFTIEAGigenpl--pltqleEMVRRNAPLL 299 Firmicutes bacterium CAG:170
SCY38221  287 frsFSGYADWTAvelkkPSFTMEFAyrqys----eadfdKIYEPAKALG 331 Alkaliphilus peptidifermentans DSM 18978
CDE72318  208 -dsAGGVKDYFIykgmgVGITVELGsdglshpiglehkdNIVKKNIGIR 255 Acidaminococcus sp. CAG:917
OYP45385  330 apaGGTWADDVEiiyqcPSVTVELGstnpvp---isefpTIFEKHKNVW 375 Prevotella sp. P4-98
CDB12524  380 -taYGTFSDYLDar-niLNCTLETGkvpapv--knsefkKIYKLNRYVL 424 Eubacterium sp. CAG:192
CDD35348  230 tlpGGGFGDWCVyekkiPSITIETGrfftpv--phwqykGIYDKNLLLL 276 Roseburia sp. CAG:309
CRZ34285  265 gdsGGNTVHYFSerfnkPAITIETVdedagfpldykyrfSTFEEIKLVL 313 thermophilic bacterium T3/55
SFI25618  200 -aaIGSFGDYFGavegvPSVTIEIGtanpvp---isqfdVIYAKNLPVW 244 Pseudobutyrivibrio sp. OR37

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