2FKM,2H4L,1P5D,2FKF


Conserved Protein Domain Family
PMM_PGM
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cd03089: PMM_PGM 
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The phosphomannomutase/phosphoglucomutase (PMM/PGM) bifunctional enzyme catalyzes the reversible conversion of 1-phospho to 6-phospho-sugars (e.g. between mannose-1-phosphate and mannose-6-phosphate or glucose-1-phosphate and glucose-6-phosphate) via a bisphosphorylated sugar intermediate. The reaction involves two phosphoryl transfers, with an intervening 180 degree reorientation of the reaction intermediate during catalysis. Reorientation of the intermediate occurs without dissociation from the active site of the enzyme and is thus, a simple example of processivity, as defined by multiple rounds of catalysis without release of substrate. Glucose-6-phosphate and glucose-1-phosphate are known to be utilized for energy metabolism and cell surface construction, respectively. PMM/PGM belongs to the alpha-D-phosphohexomutase superfamily which includes several related enzymes that catalyze a reversible intramolecular phosphoryl transfer on their sugar substrates. Other members of this superfamily include phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphomannomutase ManB, the bacterial phosphoglucosamine mutase GlmM, and the phosphoglucomutases (PGM1 and PGM2). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.
Links
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Statistics
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PSSM-Id: 100091
Aligned: 93 rows
Threshold Bit Score: 379.165
Created: 21-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2FKM_X; Pseudomonas aeruginosa PMM/PGM binds alpha-D-glucose 1,6-bisphosphate and Zn; defined at 3.5A contacts.
  • Structure:2H4L_X; Pseudomonas aeruginosa PMM/PGM binds ribose 1-phosphate and Zn; defined at 3.5A contacts.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1          # #  #                                                                        
2FKM_X        12 IFRAYDIRGVVGd-------tLTAETAYWIGRAIGSEslar--------gepCVAVGRDGRLSGPELVKQLIQGLVDCGc 76  Pseudomonas aer...
EAM51160       9 LQNGSDIRGVALegipgesvnLTPEIAKILGQAFISWlekklg---ksasklIISLGRDSRLSGPDLLAAASEGISLLGs 85  Crocosphaera wa...
XP_001415757   6 LQNGSDIRGVALdgvegeaitLDAATASAIGRAFADWlrarnp----nakdiSVGVGRDPRLSGETLRDGMFAGMVEGGa 81  Ostreococcus lu...
EDK32851       8 LQNGTDIRGIATknpe-kevnLTVERVKLITRGFIKWiknkkn---lennkiKIAIGIDSRLSGLEFKRCICEELLNLGc 83  Clostridium klu...
XP_001695635   1 LQNGSDIRGIALegvpnepvtLSAGAVFFIGVAFARWlrskg------hsapKVSVGRDPRLSGPLLESAFAAGLIHGGa 74  Chlamydomonas r...
ZP_02080960    8 FKSGTDIRGVASegvagqkinLTDEVMEKISAGFVLWlskkan---kpadqlIISVGHDSRISASRIQAAVTRALEPAGv 84  Clostridium lep...
EDQ62583      24 LQNGSDIRGVAVdgvegepvnLTEAVAEAIGAAFAAWlldkkna--dfstklRVSLGTDSRVSAKSIQSAVARGISGAGl 101 Physcomitrella ...
YP_001088833   7 LQNGTDIRGIAYends-kevnLTVEEVKKIAKAFHIWlkekt-----kkdkvTVAIGTDSRITGSQFRSTVIETLTNDDc 80  Clostridium dif...
NP_568350     60 LQNGSDIRGVAVtgvegepvsLPEPVTEAIAAAFGQWllhkkk---aesrrlRVSVGHDSRISAQTLLEAVSRGLGVSGl 136 thale cress
NP_814428      7 LQNGSDIRGIALdtee-qtatLTATAVAEIAVGVVRWlqdkkqlprkaqqrlTIAIGHDSRLTAEPIKQALVDTFLSLGi 85  Enterococcus fa...

Feature 1                                             ###       #                                
2FKM_X        77 -qVSDVGMVPTPVLYYAANVle------gkSGVMLTGDHNPPDYNGFKIVVaGETLANEQIQALRERIEKndla------ 143 Pseudomonas aer...
EAM51160      86 -tVYNFEIASTPAMFMSTINpdl----scdGAIMLTASHLPFNRNGLKFFTkNGGLNKGDITEILNLAENnnfpv----- 155 Crocosphaera wa...
XP_001415757  82 -rVIDMGLATTPACFMACVTega----kydGSVMLTASHLPFNRNGMKFFTsEGGLDKPDITDICARAAAyveagv---- 152 Ostreococcus lu...
EDK32851      84 -yVYDCGLCTTPAMFMTTVTgsy----rchGAIMITGSHLPYYYNGLKFFTeSGGCEKEDIKYILNRALNkydns----- 153 Clostridium klu...
XP_001695635  75 avVHLFGLATTPAMFYSIVLsgergdegggGGDYIHDYHLPYNANGLKFFVaAGGLDKPDIAELLQTAAAaaaeagshll 154 Chlamydomonas r...
ZP_02080960   85 -tVYDCGLASTPSMFMTTVDlk------cdGAVQITASHHPFNRNGLKFFTpDGGLESDDITALLALAEKgdkpe----- 152 Clostridium lep...
EDQ62583     102 -dVIEYGMASTPAMFNSTLTvrddlfcpvqGAIMITASHLPYNRNGLKFFTsDGGLGKKDITDILSRAEKiyhvmsnest 180 Physcomitrella ...
YP_001088833  81 -nVIDCEIATTPAMFMTTIMdgy----nsdGAIMITASHLPYYYNGLKFFTeSGGLEKTDIKEMLDIAVKndsvdy---- 151 Clostridium dif...
NP_568350    137 -dVVQFGLASTPAMFNSTLTedesflcpadGAIMITASHLPYNRNGFKFFTsDGGLGKVDIKNILERAADiykklsden- 214 thale cress
NP_814428     86 -qVIDVGLATTPAMFMATQFptl----qcdAAIMITASHLPYYFNGLKFFTaEGGAEKEDIRYILSHTDPlta------- 153 Enterococcus fa...

Feature 1                                                                                        
2FKM_X       144 -----sgvGSVEQVDILPRYFKQIRDDIama-----------kpmKVVVDCGNGVAGVIAPQLIEALgc-svIPLYCEVD 206 Pseudomonas aer...
EAM51160     156 ----gtekGNIKDYDFISVYSEGLVKKVregvnhtenfeqplkglKIIVDAGNGAGGFYAEKVLKPLgadteGSQFLDPD 231 Crocosphaera wa...
XP_001415757 153 --gsvtapSGVVSAPFLPTYAAQLCDIIrkgvnftdnydkpltgmKIVVDAGNGSGGFFADMVLEALgadtsGSQFLDPN 230 Ostreococcus lu...
EDK32851     154 ----vhsrGNIFKIDFIDEYSGILVKKIreginskvnynrplselKIIVDAGNGAGGFFANKVLEPLgakieGSQFIIPD 229 Clostridium klu...
XP_001695635 155 avslsldpARLARLPFLPTYSASLRDLIkrgvnspanyhfpllgcHVVVDAGNGAGGFFAEQVLAPLgadtsGSVFLDPD 234 Chlamydomonas r...
ZP_02080960  153 ----nvpgGSVKPVAYMRDYAKHLREIIckgvnaad-ydhplkgfHIVVDAGNGAGGFYASDVLEPLgadvaGSQFLDPD 227 Clostridium lep...
EDQ62583     181 eaaaavadKIVHSVDYMEQYTADLVDAVrkgaggv---erplegfHIVVDAGNGAGGFFAGRVLEPLgavttGSQFLDPD 257 Physcomitrella ...
YP_001088833 152 --eevnkkGEVIVKNLIEDYSNLLIDKIrkgvnssknyekpfsglKILVDAGNGAGGFFAEKVLHILgadttGSQFLNPD 229 Clostridium dif...
NP_568350    215 lrksqresSSITKVDYMSVYTSGLVKAVrkaagdl---ekplegfHIVVDAGNGAGGFFAAKVLEPLgaitsGSQFLEPD 291 thale cress
NP_814428    154 -----nenGTLMKQELLPIYAEHLVEKIrqgihspe--ekplqgfRIIVDAGNGAGGFFAEQVLQVLgadttGSQFLEPD 226 Enterococcus fa...

Feature 1                                          # # ##                                     #  
2FKM_X       207 GNFPNHHPDPgKPENLKDLIAKVkaenADLGLAFDGDGDRVGVVTntgTIIYPDRLLMLFAKDvvsrnpGADIIFDVKCT 286 Pseudomonas aer...
EAM51160     232 GTFPNHIPNPeNEEAMESICRAVidnkADFGIIFDTDVDRGAAVDnfgKELNRNRLIALISAMilkehpGSTIVTDSITS 311 Crocosphaera wa...
XP_001415757 231 GNFPNHSPNPeDKEAMAAGVQCVlnakADLGIVFDTDVDRSAVIDssgKEINRNKLIALLSEIvlkespGATIVTDSVTS 310 Ostreococcus lu...
EDK32851     230 GKFPNHLPNPeDKEAMHSIKEAViknkADLGIIFDADVDRAAIVDcsgREINRNALIALISTIvleeypGTTIVTDSVTS 309 Clostridium klu...
XP_001695635 235 GTFPNHPPNPeHPAAMASGAAAVkasaAELGIVFDTDVDRSAIVDasgREINSNRFIALMAAVvlrqhpGTTVVTDSVTS 314 Chlamydomonas r...
ZP_02080960  228 GMFPNHIPNPeNEEAMASICEAVkeqkADLGVIFDTDVDRGGAVDhqgEEINRNRLVAVASAIalegneGGTIVTDSITS 307 Clostridium lep...
EDQ62583     258 GMFPNHIPNPeDKKAMESITKAVldqkADLGIIFDTDVDRAAAVDssgKELNRNRLIALLAAIvleqhpGTTIVTDSVTS 337 Physcomitrella ...
YP_001088833 230 GMFPNHIPNPeNKEAMESICKAVldnkSDLGIIFDTDVDRAAIVGkngKPINKNALIAVISSIvleehpKTAIVTDSITS 309 Clostridium dif...
NP_568350    292 GMFPNHIPNPeDKAAMEAITKAVldnkADLGIIFDTDVDRSAAVDssgREFNRNRLIALLSAIvleehpGTTIVTDSVTS 371 thale cress
NP_814428    227 GHFPNHVPNPdNSEAMKSIQTAVlanqADLGIIFDTDVDRSAVVDqsgEVLNRNNLIAVLAAIvlkeapGSYIVTNSPTS 306 Enterococcus fa...

Feature 1                           ###                     # # #                                
2FKM_X       287 RRLIALISGy-GGRPVMWKTGHSLIKKKMKE-----TGALLAGEMSGHVFFKerwFGFDDGIYSAARLLEILSqd----- 355 Pseudomonas aer...
EAM51160     312 DGLSEFITQelNGVHHRFRRGYKNVINEAIRlnnegQESWLAIETSGHGAMKe-nYFLDDGAYLVTKLLIKLAncqq--- 387 Crocosphaera wa...
XP_001415757 311 DGLHKFITAk-GGKHVRFMRGYKNVINKGKElnasgVVTPLMIETSGHGAMKe-nYDLDDGAYLAVKIIIEAVrrrl--- 385 Ostreococcus lu...
EDK32851     310 DGLSEFIQEl-GGKHHRFKRGYRNVINEGIRlnnqnEGCYLAIETSGHAAFKe-nYFLDDGAYLIAKVLIKMArlks--- 384 Clostridium klu...
XP_001695635 315 NGLTDFITAl-GGKHMRYKRGYKNVIGAGVRlnaqgEDCALMMETSGHGALRe-nFFLDDGAYLAVKAIIEHVrrkqe-- 390 Chlamydomonas r...
ZP_02080960  308 SGLKSYIEEtlGGKHHRFKRGYKNVINEAIRlnqegINCPLAIETSGHAALRe-nYFLDDGAYLVTKIIIKMAqlkk--- 383 Clostridium lep...
EDQ62583     338 DGLTTFIEQklGGKHHRFKRGYKNVIDEGIRlntvgEETHLAIETSGHGALKe-nHWLDDGAYLMVKLLIKLAaakvsge 416 Physcomitrella ...
YP_001088833 310 EGLAKFINEl-QGRHHRFKRGYKNVINEAIRlnsegEECHLAIETSGHAALKe-nYFLDDGAYLIAKILIKVAklsl--- 384 Clostridium dif...
NP_568350    372 DGLTSFIEKklGGKHHRFKRGYKNVIDEAIRlnsvgEESHLAIETSGHGALKe-nHWLDDGAYLMVKILNKLAaaraagq 450 thale cress
NP_814428    307 SHLKTFIEEk-GGQQIRYISGYRNVINKMIElnhggFQTPLAIETSGHAAFQe-nYNLDDGAYVVAKILMLLPelkq--- 381 Enterococcus fa...

Feature 1                                                                                        
2FKM_X       356 ---qrDSEHVFSAFPSDISTPEINITvte-------dsKFAIIEALQRdaqwge----------gnittLDGVRVDYp-- 413 Pseudomonas aer...
EAM51160     388 --egkNLAEFIAKLREPEESQEFRVKintes---fqdyGNQVIEKLKEfvitqdd-------wqlvpnnYEGVRISCcsg 455 Crocosphaera wa...
XP_001415757 386 -anepSIGQVLETLEEPLEEAEVRLKiadpd---fkayGLNVIENLLAavndpahslf--gkckpapdnYEGLRVCVdeg 459 Ostreococcus lu...
EDK32851     385 --egkSIEDLIQNMKMPVESLEFRFDild-------enFKEYGDKILEelklyiekv---egwsevspnYEGIRINCcsf 452 Clostridium klu...
XP_001695635 391 -gaagGLAELLAGLAEPAESREWRVRiqh-------tdFKAVGGRVLAafhdwgavpaapsswslesvnHEGWRVNMdeg 462 Chlamydomonas r...
ZP_02080960  384 --egkTLESLLAPLQEPKESVEVRMPilted---fracGEQVISGLEAyakvhdg-------wqiapdnHEGIRVSFgkd 451 Clostridium lep...
EDQ62583     417 asgskVLSDLIADLEDPAISSELRFKidqshpdigsggFKAYGEQVLDkldqlvasd---anlkkapvnHEGIRVSGy-- 491 Physcomitrella ...
YP_001088833 385 --egkTIEELIENLEEAQEEKEVRIGink-------edFRPYAEDILKdldshvksi---dgwsvapnnFEGIRVNCdes 452 Clostridium dif...
NP_568350    451 gsgskVLTDLVEGLEEPKVALELRLKidknhpdlegsdFREYGEKVLQhvsnsietn---pnliiapvnYEGIRVSGf-- 525 thale cress
NP_814428    382 --nnqTLGDLIATLKQPAETNEFRFKitaed---vtcyGQQVLRDFELfvenqad-------favdrenQEGVRGNVsgq 449 Enterococcus fa...

Feature 1                 # ###                           
2FKM_X       414 ---kGWGLVRASNTTPVLVLRFEAdteeeLERIKTVFRNQL 451 Pseudomonas aeruginosa
EAM51160     456 -denGWLLLRLSLHDPVIPLNVESnvsggVKKIANRVLEFL 495 Crocosphaera watsonii WH 8501
XP_001415757 460 dgrqGWFLLRCSLHDPVMVLNFESqtqcgVQTMAEEFGTWI 500 Ostreococcus lucimarinus CCE9901
EDK32851     453 -kgsGWLLLRLSLHEPVLCLNIECdlsgySNIIIDKLKSFL 492 Clostridium kluyveri DSM 555
XP_001695635 463 egrrGWLLLRQSLHDPLLVLNVESelpggAAEAAVRVAAWL 503 Chlamydomonas reinhardtii
ZP_02080960  452 -egdGWFLLRLSVHDPIMPLNIESdavggVKLIAGKLYDYV 491 Clostridium leptum DSM 753
EDQ62583     492 ---gGWFLLRLSLHDPVLPLNIEGpsmeaTKKLGEFVLSAV 529 Physcomitrella patens subsp. patens
YP_001088833 453 -ngdGWFLLRISLHEPLLALNIESnkiggAEQIYSKLKLFL 492 Clostridium difficile 630
NP_568350    526 ---gGWFLLRLSLHDPVLPLNIEAqseddAVKLGLVVATTV 563 thale cress
NP_814428    450 -ygsGWFLLRLSLHEPLLVLQVENdqsdkNACVIEKIATFL 489 Enterococcus faecalis V583

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