4P5O,2EDI,3O2U


Conserved Protein Domain Family
UBCc_UBE2F_UBE2M

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cd23794: UBCc_UBE2F_UBE2M 
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzymes E2 F, E2 M and related proteins
The E2F/E2M subfamily includes mammalian ubiquitin-conjugating enzymes E2 F (UBE2F/NCE2, EC 2.3.2.32) and E2 M (UBE2M/UBC12, EC 2.3.2.34), yeast NEDD8-conjugating enzyme UBC12 (EC 2.3.2.24), plant RUB1-conjugating enzyme 1-2 (RCE1/UBC12 and RCE2/UBC12L, EC 2.3.2.-), and similar proteins. UBE2F (also called EDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, or RING-type E3 NEDD8 transferase UBE2F) and UBE2M (also called NEDD8-conjugating enzyme UBC12, or NEDD8 carrier protein) accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. The RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. UBE2M is involved in cell proliferation. Saccharomyces cerevisiae UBC12 and Arabidopsis thaliana RCE1/RCE2 accept the ubiquitin-like protein NEDD8/RUB1 from the UBA3-ULA1 E1 complex and the ECR1-AXR1 E1 complex, respectively.
Statistics
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PSSM-Id: 467414
Aligned: 151 rows
Threshold Bit Score: 83.3806
Created: 10-Aug-2017
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1: active site cysteine [active site], 1 residue position
Conserved feature residue pattern:CClick to see conserved feature residue pattern help
Evidence:
  • Comment:forms a thioester with ubiquitin or ubiquitin-like proteins
  • Comment:serves as the point of attachment of ubiquitin prior to transfer to cellular target
  • Citation:PMID 9497353
  • Citation:PMID 9705497

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
4P5O_I        29 AAQLRIQKDINELNlp------kTCDISFSDpd-----------------------------------------dLLNFK 61  human
ELR14841       4 ALLSRIYKDIIDIPhfp----tvRIKCIFETreekrerrrierakhnlshpleqlpvdessdessseddedldldIKALK 79  Acanthamoeba ca...
KII63498      28 PAFHRLKKEFTANPdt-----vqTYQIKHEDqs-----------------------------------------dPLKYT 61  Thelohanellus k...
OIR58305       3 PAAYRIQKDLNDMKlp------pSIKVSLQK--------------------------------------------LTEIH 32  Amphiamblys sp....
OMJ78051      17 VASLRLKKDMEELAskrylagytNVNISIPYiddi------------------------------------yicvPVVFH 60  Stentor coeruleus
XP_001027688  51 GYHRYLVNEYINLSkqnl--cdeTCSVYTQGysf--------------------------------------eekNLQMR 90  Tetrahymena the...
XP_001452616  18 QSFKRLTNDLLDEKlqdl--ndnTCQILINQn-------------------------------------------CDKLY 52  Paramecium tetr...
XP_001455685  16 CSLRRLQIDMLDHKlnql--nddTCEITIKMd------------------------------------------kDNILQ 51  Paramecium tetr...
XP_001425956  16 QSLKRLQNDMLDEKlqef--dddTCEINFKMv-------------------------------------------SSILQ 50  Paramecium tetr...
ETO36693      22 PGLLRVQVDLDELElp------yNAKLQVPDke-----------------------------------------nLQEFN 54  Reticulomyxa fi...
Feature 1                                                             #                          
4P5O_I        62 LVICPDEgfykSGKFVFSFKVGq--GYPHdPPKVKCEt-MVYHPSIDl-EGNVSLNILRe-DWKPVLtINSIIYGLQYLF 136 human
ELR14841      80 VIITPLGgpwkHASVPFRFDIPk--TYPNdPPKLTCLk-RIYHPSIDl-NGRVCLGLIN--EWMSILnFESIIIELEQLL 153 Acanthamoeba ca...
KII63498      62 MVITPVSgyykDQVYDFLVDIPl--NYPYdPPRVYCLk-KIFHPNIDskTKKVCSHTFGl-QWMPTYdVNSVTAAINNLL 137 Thelohanellus k...
OIR58305      33 VEIDPSTgpysGCTLPFLVRIPe--MYPHcPPRVFCTk-RVYHPNIDa-DGPVCLSILNi-NWRPFYtLETVFVSLLNTI 107 Amphiamblys sp....
OMJ78051      61 VKSDCDSpy-sNANFNTIIKVSs--NYPFkPPEMFVLn-KVYHPNVDldTGAVHMRALQehEWKPVLtLNSIIFAFETLL 136 Stentor coeruleus
XP_001027688  91 IIIQPHKg--fFASFAYQINIIatrQYPYhPLIVQSQk-KIFHPNFDqiTNNLYLEDLDfqKWNPQTsLYMLILKIKWLF 167 Tetrahymena the...
XP_001452616  53 LQITPKIgsfiYNNYQFQLDFRi--NYPYnPPLIHVEs-NIPHPNIDlkNHQLYVKVLDpnVWKPVYgLYDIIQAIKQTI 129 Paramecium tetr...
XP_001455685  52 IQMRPKIgpykLKNYLFLLDFRk--NYPIsPPIITIGs-ETLHPNIDrkNQKFYLRLIEqqNWKPIYgLFEIVEEMKKTL 128 Paramecium tetr...
XP_001425956  51 IVMIPKIgpygFKKFFFFLDFRi--NYPYsPPKIQAEs-DIPHPNIDrrTQTFYLQLLEpqNWRPIYgLTDIIKAMKQTL 127 Paramecium tetr...
ETO36693      55 VAITPDSgfwkGATYTFHFVIPd--NYPYkPPKVTCVekVNFKKNLFf-YKKKGCYHYY--AFLFCH-FYFLILSKMNWA 128 Reticulomyxa fi...
Feature 1                                                                         
4P5O_I       137 LEPNp---------------EDPLNKEAAEvlq--------------------nnRRLFEQNVQR 166 human
ELR14841     154 AQPSp---------------SKPLVKDVGNell--------------------dhPDAFAENVER 183 Acanthamoeba castellanii str. ...
KII63498     138 LEPNf---------------NEPLNNAYIStfk-------------------rttENEFQSDAFQ 168 Thelohanellus kitauei
OIR58305     108 LEPSv---------------VDPLNSTIAEhmq--------------------ndHGRFLEDLEK 137 Amphiamblys sp. WSBS2006
OMJ78051     137 NEPDv--------------dLVPCNPINTDmih--------------------vyITDYEKFYER 167 Stentor coeruleus
XP_001027688 168 LEPK----------------IDYIPENSHDccls-----------------yiydKEDFKRKLND 199 Tetrahymena thermophila SB210
XP_001452616 130 LYIDyty-----------ipNDIQCIKSAQrmgqq---------------dhstdEEESEDKGFE 168 Paramecium tetraurelia
XP_001455685 129 IHVDfti-----------ipNETICLKMAQqiln-----------------qnqlDNECENQINS 165 Paramecium tetraurelia
XP_001425956 128 IYVDfth-----------ipNEATCLLMAQkilqqnqtlqeidfelihfeisdnfKINFELNANE 181 Paramecium tetraurelia
ETO36693     129 LFGGsgwsaqythcckckknLCVHNKFFMKcllel---------------vesglASNFVRTTNH 178 Reticulomyxa filosa

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