2B69


Conserved Protein Domain Family
UGD_SDR_e

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cd05230: UGD_SDR_e 
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UDP-glucuronate decarboxylase (UGD) and related proteins, extended (e) SDRs
UGD catalyzes the formation of UDP-xylose from UDP-glucuronate; it is an extended-SDR, and has the characteristic glycine-rich NAD-binding pattern, TGXXGXXG, and active site tetrad. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.
Statistics
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PSSM-Id: 187541
Aligned: 24 rows
Threshold Bit Score: 470.195
Created: 17-May-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2B69_A     28 KRILITGGAGFVGSHLTDKLXxdGHEVTVVDNFFTGRKRNVEHwigheNFELINHDVVEPLy-----iEVDQIYHLASPA 102 human
YP_594157   1 MKVLLTGSAGFVGSHLAERLLraGHHVTGVDNYLSGQRRNTELlrahpHFRFVEADVSAGLpv---dgSFDAVLHFASPA 77  Deinococcus geothe...
YP_843502  24 KNILVTGGAGFLGSWICDALIaqGANVVCVDNLSSGLISNISHlldadRFEFIQHDVSDLSrplkidqKLDLVIHMASRA 103 Methanosaeta therm...
NP_108106  24 RRALVAGGAGFLGSHLCERLLrdGYDVVALDNFHTGKRYNLNTllrdpRFTCIEHDIVDPLpa---glEVDEIYNLACPA 100 Mesorhizobium loti...
YP_822649   1 MRIVVSGSAGFVGSHMCERLLneGHSVVALDNFLTGSPANLAHlekhpRLQFVEQDITRPFtv---dgAVDCVVNMASPA 77  Solibacter usitatu...
NP_865691  17 QRILVTGGAGFLGSHLCERLVsdGHDVICLDNFFTSQKTNVVHlldkpNFELIRHDITLPIh-----lEVDQIYNMACPA 91  Rhodopirellula bal...
NP_579086  24 KTALVTGGAGFLGSWLCDVLIelGAKVYCVDNFASGRWENISHltseeNFVFIEHDVSKPLei---reKLDFIFHFASRA 100 Pyrococcus furiosu...
XP_820252   8 EVVLITGGSGFIGSHVVDRAMreGYTVVAVDNHYTGREQNIAHhidkeNFHFVKHDVRHPYpeevlrhKYNYIFHLASPA 87  Trypanosoma cruzi ...
XP_667446   4 ITVLVTGASGFIGSHLVEYLLskGYYVLALDNFFSGDVINIGQcrdnpRLEIIRHDIIDSIk-----lEVKEIYHLACPA 78  Cryptosporidium ho...
BAE57067   22 LKILITGAAGFLGSNLADYLLakGQVVIGMDSFQTGSPQNLEHlrnhpDFTFVNQNIQLPLed---vgQIDQIYNLACPA 98  Aspergillus oryzae
Feature 1                    #                      #                            #   #        
2B69_A    103 SPPNYXYNPIKTLKTNTIGTLNXLGLAKRVGARLLLASTSEVYGDPEVHPQSEDYWGHVNPIGPRACYDEGKRVAETXCY 182 human
YP_594157  78 SPPHYQQHPVETLMVGAQGTQHALELARRCGATFLLASTSEVYGDPRVHPQPESYWGHVNPTGLRSCYDEAKRYAEALTM 157 Deinococcus geothe...
YP_843502 104 SPFEFEHYPIEILKANTIGLMASLDIARNHDARLLYTSTSEVYGNPTVVPTPESYNGNVNPIGPRGCYDEAKRCGEAYVM 183 Methanosaeta therm...
NP_108106 101 SPAHYQADPIHTFKTSVLGSLNLLELARRSNAKIFQASTSEVYGDPLVHPQPESYFGNVNTHGPRSCYDEGKRSAETLFF 180 Mesorhizobium loti...
YP_822649  78 SPKDYLEYPIETLDVGSIGSRNMLELALAKGARYLVTSTSECYGDPMVHPQVETYWGNVNPVGPRSCYDESKRFAEAITM 157 Solibacter usitatu...
NP_865691  92 APGHYQFNPIKTIKTSVMGSINMLGIAKRCGARILQASTSEVYGDPEQHPQTESYRGSVNPIGIRACYDEGKRVAETLFM 171 Rhodopirellula bal...
NP_579086 101 SPFEFEHYPLEIIDANTLGTRNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVNPIGIRSCYDESKRLGEALTM 180 Pyrococcus furiosu...
XP_820252  88 SPVHYQADPIGTTLTCVNGTYHSLLLAQRDDCPVLIASTSEVYGDPIQHPQTEEYWGNVNCTGVRSCYDEGKRCAESLCF 167 Trypanosoma cruzi ...
XP_667446  79 SPIHYQKDPIYTLKTCFIGTMNILGLAKRTNSKVVFASTSEIYGDPLVHPQNESYYGNVNTVGTRSCYDEGKRIAETLCM 158 Cryptosporidium ho...
BAE57067   99 SPIQYQKDPISTLRTCFQGTQNVLDLAISKNARVLHTSTSEVYGDPLVHPQPETYWGNVNPFGMRSCYDEGKRVAEALCY 178 Aspergillus oryzae
Feature 1                                                                                     
2B69_A    183 AYXKQEgVEVRVARIFNTFGPRXHx--NDGRVVSNFILQALQGEPLTVYGSGSQTRAFQYVSDLVNGLVALXNSnv---- 256 human
YP_594157 158 AYHRHHgVDTRIVRIFNTYGPRMRa--DDGRVVTNFINQALAGRPLTVYGDGQQTRSFQYVDDLVEGIMRLLASay---- 231 Deinococcus geothe...
YP_843502 184 AYRNQYgLDVRIARIFNTYGPRIRwdcIYARAVPRFIAQAIRGEPITIFGDGTQTRSFTYVTDQIEGLLRLASIdev--- 260 Methanosaeta therm...
NP_108106 181 DYSRTYgLDIRVARIFNTYGRRMQp--DDGRVVSNFIVQALRGEDLTVYGSGLQTRSFCYADDLIEGFIRLMNApha--- 255 Mesorhizobium loti...
YP_822649 158 AYHRKHgVRTNIARIFNTYGPRMKl--DDGRVVPAFLDQALRGEPMTVFGTGSQTRSFCYVSDLVDGLYRLMQSde---- 231 Solibacter usitatu...
NP_865691 172 DYHRSNnVDVRIVRIFNTYGPRMHp--FDGRVVANFIRQALAGDDITIFGDGSQTRSFCYRDDLVEVIIRMMNCdg---- 245 Rhodopirellula bal...
NP_579086 181 AYYRQFnVDVRIVRIFNTYGPRMRadgVYGRVVPRFISQALNEEPITVFGDGSQTRSFCYVTDLITGVLKFAAVeng--- 257 Pyrococcus furiosu...
XP_820252 168 DFHRKHgVKIRVARIFNTYGPRMCf--NDGRIISNFLIQSLRGEDITVYGTGTQTRSFQYCDDLVEGFFRLIRHpt---- 241 Trypanosoma cruzi ...
XP_667446 159 EYYRSHgVDVRIARIFNTYGPKMLf--NDGRVVSNFILSSLLNQELPIYGDGTQTRSFCYVTDMVYGLYKLMKLdrekil 236 Cryptosporidium ho...
BAE57067  179 AYREQQgADIRIARIFNTYGPRMNg--SDGRVVSNFIVAALSGEDLKITGDGTATRSFQYVTDCMKGLYRLMNSdy---- 252 Aspergillus oryzae
Feature 1                                                                                     
2B69_A    257 -sSPVNLGNPEEHTILEFAQLIKNLVG-----SGSEIQFLseaqDDPQKRKPDIKKAKLxLGWEPVVPLEEGLNKAIHYF 330 human
YP_594157 232 -hGPVNIGNPDEYTILEFAQVIRELID-----PGLEIVHApmpaDDPRQRRPDISLARElLGWEPRVSLLDGLRRTVAHF 305 Deinococcus geothe...
YP_843502 261 kgAVVNIGNDRETMIIELAKIVLKITG-----SDSGIVYQplpeDDPLRRCPDITKARElLGWAPKVALEDGLRRTVEWF 335 Methanosaeta therm...
NP_108106 256 paHPVNLGNPGEFTIMELATLVVGYTN-----SRSKIVHRplpiDDPRQRKPDISFARDnLGWEPRINLAQGLAHTVDYF 330 Mesorhizobium loti...
YP_822649 232 -rYPVNLGNPREMTILEFAEHIRAMTG-----TKSEIIFHplpeDDPKQRKPDITKARSvLGWEPRISLEDGLRDTVEYF 305 Solibacter usitatu...
NP_865691 246 fiGPVNIGNPHEFTIRQLAEKTIELTG-----SSSKLIEAplpaDDPTRRRPDIALAKEkLDWEPKIELEQGLKHTIDWF 320 Rhodopirellula bal...
NP_579086 258 rgEVVNLGNPREISILELAYLIKKLTN-----SDSPIEFHplppDDPPRRCPDISKAQKlLNWKPKVELEEGLKKTIKWF 332 Pyrococcus furiosu...
XP_820252 242 eiGPVNLGNPDEYTVLDMAKKVRDFVPg----TKSNICFLspceDDPKQRCPDISKARRvLGWTPVVPLSEGLRRTAEDF 317 Trypanosoma cruzi ...
XP_667446 237 dnMPINLGNPNEISILELGEVIRELIN-----PNLKISHRkfpmDDPKKRQPDISRAIGiLNWKPTVDIKTGIKETIKDF 311 Cryptosporidium ho...
BAE57067  253 seGPVNIGNDGEFTIQQLAEKVAGLVAemtnqPKVNITYHprpaDDPAVRRPQISLAKAvLNWCPTIPLQEGLRRTIEWH 332 Aspergillus oryzae
Feature 1      
2B69_A    331 R 331 human
YP_594157 306 Q 306 Deinococcus geothermalis DSM 11300
YP_843502 336 R 336 Methanosaeta thermophila PT
NP_108106 331 D 331 Mesorhizobium loti MAFF303099
YP_822649 306 R 306 Solibacter usitatus Ellin6076
NP_865691 321 K 321 Rhodopirellula baltica SH 1
NP_579086 333 G 333 Pyrococcus furiosus DSM 3638
XP_820252 318 A 318 Trypanosoma cruzi strain CL Brener
XP_667446 312 K 312 Cryptosporidium hominis TU502
BAE57067  333 V 333 Aspergillus oryzae

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