5NW4,6F1T,6F38,7Z8F,7Z8I


Conserved Protein Domain Family
ASKHA_NBD_Arp1

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cd10216: ASKHA_NBD_Arp1 
nucleotide-binding domain (NBD) of actin-related protein 1 (Arp1) and similar proteins
Arp1, also called centractin, actin-like protein, alpha-centractin, actin-RPV, or centrosome-associated actin homolog, may be a component of a multi-subunit centrosomal complex involved in microtubule-based vesicle motility. In yeast, actin-related protein is essential for viability and is associated with the centrosome. In vertebrates, Arp1 is a core component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. ARP1 forms the backbone filament of the dynactin rod structure and serves as the scaffold for the remaining subunits. It is required for proper orientation of the mitotic spindle. Arp1 is the only actin-related protein known to form actin-like filaments. Human Arp1/centractin is encoded by the ACTR1A gene.
Statistics
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PSSM-Id: 466820
Aligned: 60 rows
Threshold Bit Score: 557.929
Created: 9-Oct-2006
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Structure:6F1T; Sus scrofa ARP1 in complex with ADP, contacts at 4A
  • Structure:7Z8F; Sus scrofa ARP1 in complex with MgADP, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #### #                                                                  
5NW4_G         9 NQPVVIDNGSGVIKAGFAGDQIPKYCFPnYVGRPKHVRVMAGALe-GDIFIGPKAEEHRGLl-SIRYPMEHGIVKdWNDM 86  pig
6F1T_A         9 NQPVVIDNGSGVIKAGFAGDQIPKYCFPnYVGRPKHVRVMAGALe-GDIFIGPKAEEHRGLl-SIRYPMEHGIVKdWNDM 86  pig
P38696        12 NQPVVIDNGSGIIKAGFSGEERPKALEYcLVGNTKYDKVMLEGLq-GDTFIGNNAQKLRGLl-KLRYPIKHGVVEdWDSM 89  Saccharomyces c...
NP_596704      9 NQPICIDNGSGFIKAGFAGDDIPKCLFPtCVGRIKHERVMPSSIq-KDMFVGSEAQNLRGLl-KIQRPIERGIIQnWSDM 86  fission yeast
O94630         9 NQPICIDNGSGFIKAGFAGDDIPKCLFPtCVGRIKHERVMPSSIq-KDMFVGSEAQNLRGLl-KIQRPIERGIIQnWSDM 86  Schizosaccharom...
CAH69672       8 NNPMIIDMGSSQIKAGFGGEDKPKVIFNsYIGRPKVKALSATNS--QELYVGNQITDSIRGnmKIKYPYQKGIIKdMNDM 85  Paramecium tetr...
EEA04879      12 SQPAIIDNGSGSIKSGFAGEEIPRCIVNsCVGRPKYKRCMAGALe-GDVFVGTKSIENRGIl-RISYPISNGIIQdWYDM 89  Cryptosporidium...
EFC40698       5 GTPLVIDNGSGLIKAGLAGDEIPNYVFNnYVGSTKHVRVMPVAGpkYDFCVGKVAEEKRGIl-KLKYPMEHGVVTdWSAM 83  Naegleria grube...
XP_009495586   6 NQFIVIDNGSFTIKAGFAGEDRPQALLRtCIGHTKYQKVMSGGDfePGRMIGNSLQEYRGLf-RTTSPIKNGLIEnWEDM 84  Fonticula alba
XP_011129506   3 NQVIVLDNGSSTIKAGFAGDVKPLVVLPsYVGRQKNERVMVDAPn-EDAYVGEQARKYRGIl-KLSYPIDRGIIKdETDL 80  Gregarina nipha...
Feature 1                                                                      #                 
5NW4_G        87 ERIWQYVYSkeql-------qtfsEEHPVLLTEAPlNPRKNRERAAEVFFEtfnvPALFISMQAVLSLYATGrTTGVVLD 159 pig
6F1T_A        87 ERIWQYVYSkdql-------qtfsEEHPVLLTEAPlNPRKNRERAAEVFFEtfnvPALFISMQAVLSLYATGrTTGVVLD 159 pig
P38696        90 ELIWSYVLNevlq-------lqniGEHPLLITEAPmNPLKNREQMAQVLFEtfdvSALYVSNPAVLSLYASGrTTGCVVD 162 Saccharomyces c...
NP_596704     87 EEIWSYIYSdqql-------ntlpEEHPLLLTEPPlANIRNKEKIAEYFYEtlnvPALSFSLQPVLALYASArTTGIVLE 159 fission yeast
O94630        87 EEIWSYIYSdqql-------ntlpEEHPLLLTEPPlANIRNKEKIAEYFYEtlnvPALSFSLQPVLALYASArTTGIVLE 159 Schizosaccharom...
CAH69672      86 DQLWKYTFQelh---------snpKECPILLTEPSfSSQIQKMEIAKQFFEnydcPALFFAVSGIVSLFASGkTTGVILD 156 Paramecium tetr...
EEA04879      90 ETLWTHIFSelk---------issEEHPVLLTEVAlNPRQHREKMAEIFFEtyrvPALFISTQAVLSLYSSGrTTGIVLD 160 Cryptosporidium...
EFC40698      84 ERVWDYLYAclmdkdtglgetfkrEDSPVLLTEAPlNPRKNREKAADIFFEsynvPKLFISLQAVLSLYASGsMTGVVLD 163 Naegleria grube...
XP_009495586  85 EALWNHLYSqhlk--------vssEDHPVLITDAAlSPHKQRLQIAQVLFEsynvPALSIAVPGVLSLYSSGmTSGIALD 156 Fonticula alba
XP_011129506  81 ELLWKEVFNqln---------vssAGHPLLISDSAlLPTSNKEKLVQIAFEsfgvPMVCVMSQTVLALYATArTTGTILD 151 Gregarina nipha...
Feature 1        ###                                                        ##                   
5NW4_G       160 SGDGVTHAVPIYEGFAMpHSIMRIDIAGRDVSRFLRLYLRKEGy-DFHSSSEFEIVKAIKERACyl-----sINPQKDET 233 pig
6F1T_A       160 SGDGVTHAVPIYEGFAMpHSIMRIDIAGRDVSRFLRLYLRKEGy-DFHSSSEFEIVKAIKERACyl-----sINPQKDET 233 pig
P38696       163 CGEGYCSTVPIYDGFALpASMMRMDIGGADITEQLQFQLRKSAgvSLFSSSEREIVRTMKEKVCyl-----aKNIKKEEE 237 Saccharomyces c...
NP_596704    160 CGDGLTHSVPIYDGFSIpSAIQQEEIGGRDVTDYLQLQLRKSGh-ELVSSAEKEIVREIKEKCCyv-----aSDFRSEIE 233 fission yeast
O94630       160 CGDGLTHSVPIYDGFSIpSAIQQEEIGGRDVTDYLQLQLRKSGh-ELVSSAEKEIVREIKEKCCyv-----aSDFRSEIE 233 Schizosaccharom...
CAH69672     157 VGDTVSQSIPIYDGFYIqHSAQRVDLAGRDVTDNLNNLLRRSGy-IFTKSAELEIIKKIKEKRCfl-----sPTMIAEEK 230 Paramecium tetr...
EEA04879     161 SGDGVTHAVPIYQGFALnNCITRSDIAGRNITEYLNLQLRRSGi-IFHTSAEMEIVKQIKETVCfv-----sSNLQKDEN 234 Cryptosporidium...
EFC40698     164 SGDGVTHCVPIYDGFAIpNAITRIDVAGRDITNYLQLLLRKSGy-NFHTSSEIEVVKSIKESDLmyvaavyeKEMIMDEE 242 Naegleria grube...
XP_009495586 157 SGNGVTAAIPVYNGFIPtGTVRRMDIGGDDITKYLQLLLRKSGy-FFNTSAELDIVREMKERLGkise-vttFNPLHMAT 234 Fonticula alba
XP_011129506 152 VGEGGTRVTSVAEGFALpHSVEATAVGGRDVTMYLQKLLKQQGl-RLETSSELEIVREIKETVCava--ataLDKKNLGT 228 Gregarina nipha...
Feature 1                                                                                        
5NW4_G       234 Le-----------------tEKAQYYLPDGSTIEIGpsRFRAPELLFRPDLIGEESEGIHEVLVFAIQKsDMDLRRTLFS 296 pig
6F1T_A       234 Le-----------------tEKAQYYLPDGSTIEIGpsRFRAPELLFRPDLIGEESEGIHEVLVFAIQKsDMDLRRTLFS 296 pig
P38696       238 Kylqg------------tqdLISTFKLPDGRCIEVGndRYRAPEILFSPQIIGLGYDGLSDMCMQSIWKvDLDLRKPLLS 305 Saccharomyces c...
NP_596704    234 Swte-------------hkpQIHTYQLPDNQTITLGteCFSAPEVLFNPEMMGSEASGLHIQLFKSILLsDIDLRSTLYS 300 fission yeast
O94630       234 Swte-------------hkpQIHTYQLPDNQTITLGteCFSAPEVLFNPEMMGSEASGLHIQLFKSILLsDIDLRSTLYS 300 Schizosaccharom...
CAH69672     231 Fqee--------------rkIKDQYMLPDNNTIEISyeKQRAPEILMSPEKYGLELNSIPEILANSIQKvDLDLRKSLYN 296 Paramecium tetr...
EEA04879     235 DenqlvnssldsgtgsqrssIGYPYKLPDGQTIFIGkeRFRAPELLFQPNLIGSEYPGLHEVLIHSINKtDMDLRRTLAS 314 Cryptosporidium...
EFC40698     243 Kgm----------------lDPKPYKLPDGSIIQLGseRYMAPEILFKPYLIGLEYMGVHECVLRSIQTsDLDIRKDLYS 306 Naegleria grube...
XP_009495586 235 Ttgsgagad---sttdsaatEPIKYLLPDNQVIEIAdePTRAIEAIFAPHVMGKESPGIHTLLAESIQLcDIDIRKSLYS 311 Fonticula alba
XP_011129506 229 Tlgg-------------aeaGAVSYTLPDGAIVKIAn-RHAAPEILFNPALDAREAHGIPQLVHQAITNcDLDLRRHLCA 294 Gregarina nipha...
Feature 1             ### ##                                                                     
5NW4_G       297 NIVLSGGSTLFKGFGDRLLSEVKKLapk-dvKIRISAPqERLYSTWIGGSILASLdTFKKMWVSKKEYEEDGarSIHRKT 375 pig
6F1T_A       297 NIVLSGGSTLFKGFGDRLLSEVKKLapk-dvKIRISAPqERLYSTWIGGSILASLdTFKKMWVSKKEYEEDGarSIHRKT 375 pig
P38696       306 SIILSGGTTTLKGFGDRMLWDLEALtkg-tsKIKIIAPsERKYTTWIGGSILTGLsTFQRLWTKKSDWLEDSt-RVYSNL 383 Saccharomyces c...
NP_596704    301 NIVLSGGSTLLRGFGERFISELRAIsgk-knQVKIYASpERMHNAWLGGSILASLsTFRRLLITSEEYKNDQnvIFRRRF 379 fission yeast
O94630       301 NIVLSGGSTLLRGFGERFISELRAIsgk-knQVKIYASpERMHNAWLGGSILASLsTFRRLLITSEEYKNDQnvIFRRRF 379 Schizosaccharom...
CAH69672     297 EIVLTGGTSLMQGFPERLIGEVKRLipk-daKVKIWAPpERITLCWQGGSILSKLaSFKSMWILKKEFEDEGerILIKKQ 375 Paramecium tetr...
EEA04879     315 QIVLSGGSTMFPGLGDRLLYELRKAlpk-dvKIRINASsDRTLLPWIGGSILGSLaTFKTIWITKQEFLEEGpnVVHRKT 393 Cryptosporidium...
EFC40698     307 NIFFAGGSTLFDGFCSRMLSELKKSapkdvpSLRISAPnERAYTTWLGGSILASLpSFSDMWITKNDYEEHGssIVHKKT 386 Naegleria grube...
XP_009495586 312 NIILSGGNTLFSGFCSRLLSEIQHSrp--tnKVRIYAAaDRESSAWVGGSILAALsSFRKMTITYAQYDEDRd-ILRRKL 388 Fonticula alba
XP_011129506 295 SIVVTGGSTLFGAFLDRFVNDFKRLqpk-daLIRAYAPvDRQYCAFMGGTVVGMLpSFKSLMISRTQYDTDGvaAVHRNF 373 Gregarina nipha...

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