1QMV,1E2Y,1KYG,1QQ2,1UUL,1WE0,2BMX


Conserved Protein Domain Family
PRX_Typ2cys
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cd03015: PRX_Typ2cys 
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Peroxiredoxin (PRX) family, Typical 2-Cys PRX subfamily; PRXs are thiol-specific antioxidant (TSA) proteins, which confer a protective role in cells through its peroxidase activity by reducing hydrogen peroxide, peroxynitrite, and organic hydroperoxides. The functional unit of typical 2-cys PRX is a homodimer. A unique intermolecular redox-active disulfide center is utilized for its activity. Upon reaction with peroxides, its peroxidatic cysteine is oxidized into a sulfenic acid intermediate which is resolved by bonding with the resolving cysteine from the other subunit of the homodimer. This intermolecular disulfide bond is then reduced by thioredoxin, tryparedoxin or AhpF. Typical 2-cys PRXs, like 1-cys PRXs, form decamers which are stabilized by reduction of the active site cysteine. Typical 2-cys PRX interacts through beta strands at one edge of the monomer (B-type interface) to form the functional homodimer, and uses an A-type interface (similar to the dimeric interface in atypical 2-cys PRX and PRX5) at the opposite end of the monomer to form the stable decameric (pentamer of dimers) structure.
Links
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Statistics
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PSSM-Id: 239313
Aligned: 88 rows
Threshold Bit Score: 183.475
Created: 4-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:Deprotonation of the catalytic cysteine thiol is facilitated by H-bonding with the threonine or serine hydroxyl group, and the positive charge of the arginine sidechain.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                            #  #                       
1QMV_A        7 IGKPAPDFKATAVVd----------gAFKEVKLSDYKG---KYVVLFFYPLDFTFVXPTEIIAFSNRAEDFRKLGCEVLG 73  human
1QQ2_A        8 IGHPAPSFKATAVMpd---------gQFKDISLSDYKG---KYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIG 75  Norway rat
P19476       43 IGKEAPEFKAPAYCpc---------gSIKEIDINEYRG---KYVVLLFYPLDWTFVCPTEMIGYSELAGQLKEINCEVIG 110 Entamoeba histol...
AAK15374      2 LNTRIKPFKNISYYk----------kKFYEIKEIDLKS---NWNVFFFYPYSYSFICPLELKNISNKIKEFKNLNTKIYA 68  Candidatus Carso...
AAK15383      2 LNTKIKPFNTISYFn----------kKFFNINLNDLKN---KWNIFFFFPYSFTFICPTELKDISNYTFKFKEKNCNIFA 68  Candidatus Carso...
AAK15391      2 LNTKINSFKSIAYFk----------kKFFEITDKELKN---FWNVLFFFSFSYTYICPTELLELSKNINLFKNLKCNIYA 68  Candidatus Carso...
ZP_00308441   1 MGSAFPAFKKTAVVsie------kgkEFTEISSENLVNednQWTVMFWWPKDFTFVCPTEIAEFNNAFGDFRDRDTTLIG 74  Cytophaga hutchi...
YP_108693     4 VGDKLEAFTVVAAKpgfnnheengqsAFETVTEASFPG---KWKIIYFYPKDFTFVCPTEIVEFAKLAKQFEERDAVLLG 80  Burkholderia pse...
YP_156957     4 VGDKLPEFSVIAAKpkfnmpeengesAFETIDNNSFEG---KWKIIFFYPKDFTFVCPTEIVEFAKLNEDFADRDAVVMG 80  Idiomarina loihi...
AAV90356      4 VGDKFPALKLPVQQgvs------alpNGETIDLGETNG---KWKVVFFWPMDFTFVCPTEIVGYNDIKEDLSLRDAVLIG 74  Zymomonas mobili...

Feature 1                                                           #                           
1QMV_A       74 VSVDSQFTHLAWINTPRkegglGPLNIPLLADVTRrLSEDYGVLKTDeGIAYRGLFIIDGKGVLRQITVNDLPVGRSVDE 153 human
1QQ2_A       76 ASVDSHFSHLAWINTPKkqgglGPMNIPLVSDPKRtIAQDYGVLKADeGISFRGLFIIDDKGILRQITINDLPVGRSVDE 155 Norway rat
P19476      111 VSVDSVYCHQAWCEADKskggvGKLTFPLVSDIKRcISIKYGMLNVEaGIARRGYVIIDDKGKVRYIQMNDDGIGRSTEE 190 Entamoeba histol...
AAK15374     69 ISNDSHFVQKNWIENEL-----KFINFPFISDFNHkISNNFNILNKKdGNCLRSTIIIDKNLIIKYINIVDDSIGRSIDE 143 Candidatus Carso...
AAK15383     69 ISTDSHYTQKKWVETEL-----NFVNFPLISDFNKnISKNFNIYNKKdGNCERSTFILDRNLNIKYIEILDINIGRSIND 143 Candidatus Carso...
AAK15391     69 ISTDSHYTHKNWIENEI-----NFINFPFISDFNHkTSKNYKILNKNdGNCYRSTFIIDPNLIIKSIEIIDISISRSIQE 143 Candidatus Carso...
ZP_00308441  75 ASTDSEFVHAAWRRDHEd---lRGLKFPMLADTSKsLAEELGILEAAeKVALRATFIIDPQGIIRWVSVNDLNVGRNVKE 151 Cytophaga hutchi...
YP_108693    81 GSSDNEFVKLAWRREHKd---lDKLNHYSFGDVKGeLIDQLGVRDKEaGVALRATFIVDPDNTIQHVSVNNLNVGRSPEE 157 Burkholderia pse...
YP_156957    81 GSTDNEFVKLAWRREHPd---lDRLNQYSFADDGS-LVDGLGVRDKAeNVALRATFIVDPHNVIQHVSVNNLNVGRNPAE 156 Idiomarina loihi...
AAV90356     75 GSCDTAFVHYAWRKSDEr---lENADFPWIADNSKkLASALGIVNEEaGVAYRATFIVDPNNVIQHVTVNSLNVGRNPAE 151 Zymomonas mobili...

Feature 1                                 
1QMV_A      154 ALRLVQAFQYTdeh-gevCPAGWKPG 178 human
1QQ2_A      156 ILRLVQAFQFTdkh-gevCPAGWKPG 180 Norway rat
P19476      191 TIRIVKAIQFSdeh-gavCPLNWKPG 215 Entamoeba histolytica
AAK15374    144 ILKNIKMLQFIntnenklCPYSWNND 169 Candidatus Carsonella ruddii
AAK15383    144 IYQKLSMIQFTnkninklCPFSWIKD 169 Candidatus Carsonella ruddii
AAK15391    144 ILNKIKMLIFTfknknklCPYNWLKD 169 Candidatus Carsonella ruddii
ZP_00308441 152 VIRVLDALQTDe-----lCPCNWEKG 172 Cytophaga hutchinsonii
YP_108693   158 ILRILDGLQTDe-----lCPCNRAIG 178 Burkholderia pseudomallei K96243
YP_156957   157 IVRILDGLQTDe-----lCPCNRALG 177 Idiomarina loihiensis L2TR
AAV90356    152 ALRVLDALQTDd-----lCPCNWHKG 172 Zymomonas mobilis subsp. mobilis ZM4

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