5LF8


Conserved Protein Domain Family
NUDIX_Nudt17

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cd04694: NUDIX_Nudt17 
nucleoside diphosphate-linked moiety X)) motif 17
Nucleoside diphosphate-linked moiety X)) motif 17 (EC 3.6.1.-) encoded by the NUDT17 gene on chromosome 1q21.1 and encodes an enzyme thought to hydrolyse some nucleoside diphosphate derivatives. Members of the NUDIX hydrolase superfamily catalyze the hydrolysis of NUcleoside DIphosphates linked to other moieties, X. Enzymes belonging to this superfamily require a divalent cation, such as Mg2+ or Mn2+, for their activity and contain a highly conserved 23-residue NUDIX motif (GX5EX7REUXEEXGU, where U = I, L or V), which functions as a metal binding and catalytic site. Substrates of NUDIX hydrolases include intact and oxidatively damaged nucleoside triphosphates, dinucleoside polyphosphates, nucleotide-sugars and dinucleotide enzymes. These substrates are metabolites or cell signaling molecules that require regulation during different stages of the cell cycle or during periods of stress. In general, the role of the NUDIX hydrolase is to sanitize the nucleotide pools and to maintain cell viability, thereby serving as surveillance & "house-cleaning" enzymes. Substrate specificity is used to define families within the superfamily. Differences in substrate specificity are determined by the N-terminal extension or by residues in variable loop regions. Mechanistically, substrate hydrolysis occurs by a nucleophilic substitution reaction, with variation in the numbers and roles of divalent cations required.
Statistics
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PSSM-Id: 467576
Aligned: 16 rows
Threshold Bit Score: 148.596
Created: 6-Dec-2006
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
NUDIX motifputative metal
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:NUDIX motif [structural motif]
Evidence:
  • Comment:G[X5]E[X7]REUXEEXGU, where U is a bulky aliphatic residue (usually Ile, Leu, or Val).
  • Comment:The NUDIX motif contains many of the active site residues of NUDIX hydrolases.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                            #######################                  
5LF8_A     91 VDLGVAVILQSSd---kTVLLTRRARTLsvSPNLWVPPGGHVELEEELLDGGLRELWEESGLHLpqgq------------ 155 human
ELR18670    6 VQIGVVVLIEAHdhdgtKVLVTRRRNDAfiFPSVWVLPGGHAEKGESLLHAGKREVEEETGVVLade------------- 72  Acanthamoeba caste...
CAK63913   79 IRLATVAMLCDKd---dNFLITRRHSQMktFPKTWVFPGGMVERLQDLESECLREVQEETGIDVspil------------ 143 Paramecium tetraur...
EEB10600   88 ISVATSVVIESSd---sHVLLTKRAQHMrtSPNVWVTPGGHIENNESVYSAGIREVQEETGLDIsng------------- 151 human body louse
EFA81789  162 IKVGVSILIEDVf---gRVFLTKRAKSMriFPSVWVLPGGHMEKGETIFETALRELKEETGLVLsdsa------------ 226 Polysphondylium pa...
EAL64607  292 VKVGVSVLVEDSy---nRILLTKRSESLriFPGIWVLPGGHMEIGENFIQTGLRELNEETGITIdmidt----------- 357 Dictyostelium disc...
GAA58138   36 IPVASACLLESPsr--dGLILTRRPRHMrsYPGQWVPPGGHCDPGEPIQLTAVRELCEELGLESdsvlvdqwk------- 106 Clonorchis sinensis
EAR88489  113 TGVAVCGIIIDKn---nYVLLTKRNPEMrtYPRCWVFPGGQVDLGESFLNTVFREIKEEVGLNIllpqsqqeenfdfeee 189 Tetrahymena thermo...
EKX51143   25 VTVAAVTLPVNSn---gDVLVTQRAFRGm-YDGMWVFPGGHVDGGEALSAAAVREVLEETGLRVvkd------------- 87  Guillardia theta C...
ACM19485   11 IVTSVVAVIVDDd---eRVLLTKRNIPP--FMDQWVMPGGQIDLGEPMLEALHREVMEEVGLEVev-------------- 71  Geobacter sp. FRC-32
Feature 1                                                                                     
5LF8_A    156 -----------------------------------------------------------------fswvPLGLWESAYpp 170 human
ELR18670   73 ------------------------------------------------------------------dmrLCGLWESCYpa 86  Acanthamoeba caste...
CAK63913  144 -----------------------------------------------------------------nkmeLKVLYESVYpt 158 Paramecium tetraur...
EEB10600  152 ------------------------------------------------------------------kisVLGLWENVYpl 165 human body louse
EFA81789  227 -----------------------------------------------------------------adisVIGAFESTFpq 241 Polysphondylium pa...
EAL64607  358 -----------------------------------------------------------------ktlqVIGIFESSYpn 372 Dictyostelium disc...
GAA58138  107 -------------------------------------------------------------erdstllhPLCAWEAAYpt 125 Clonorchis sinensis
EAR88489  190 feqqspknqqntieekeggieeeqenmfkrqtrlqkkiqdqkkkvetnqqmkkedfltksavhlmedpkPLFLYESVFpt 269 Tetrahymena thermo...
EKX51143   88 ------------------------------------------------------------------slrPLAVWEGTVt- 100 Guillardia theta C...
ACM19485   72 -------------------------------------------------------------------qgLVDVFEHLTp- 83  Geobacter sp. FRC-32
Feature 1                                                                      
5LF8_A    171 rl-swgLPKYHHIVLYLLVisqesq-------qqlqarIQPNPNEVSALMWLTpdvAAAVAAAED 227 human
ELR18670   87 qv-dhgPMKKHHLVVFYRAivhkp---------ttdlsLHLQEEEVDGACWMSmakLAEWIEVEE 141 Acanthamoeba castellanii str. Neff
CAK63913  159 klqagqLPQKQTLCIFYEVklnet---------cnniqVKIQETEVDDFKWIPkkqLLEIMNAQT 214 Paramecium tetraurelia
EEB10600  166 ll-gigSPKNHNLVVYTHVlldenh-------keltnkIKPDINEVDSFCWLHgsvILQMTKTKS 222 human body louse
EFA81789  242 yldddrLPSDHHVVLFTKIklhsdi--------snlqkVELEPDEVEVAAWVPtssLAHLLTDIS 298 Polysphondylium pallidum PN500
EAL64607  373 llpvdkLPIDHHIVVFIYVklalptnnn--nnnnnkviIKLQESEVQLAAWTPkslLLKLLEGTH 435 Dictyostelium discoideum AX4
GAA58138  126 ----ltTPKSHHLVIYYAVmwpqldhpqsktsdpclpqLNIQEDEVSSAVCLPisvLQSLVNDWT 186 Clonorchis sinensis
EAR88489  270 di-kinYPQRSSLVIFYALkikes---------fkdiqLSQQRKEIDYSAWIPlelLNKLIEENA 324 Tetrahymena thermophila SB210
EKX51143  101 ------SKKRQFCVVFFAAdvsg-----------nsenMYLQTKEVHRATWIPadlLPRMLDTHV 148 Guillardia theta CCMP2712
ACM19485   84 -----gPHNSHFVILYYRCrpl-------------ycdITHNPQEVAEARWVPcgeLARYDMPDG 130 Geobacter sp. FRC-32

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