3KWP,5HW4


Conserved Protein Domain Family
RsmI
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cd11648: RsmI 
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Ribosomal RNA small subunit methyltransferase I (RsmI), also known as rRNA (cytidine-2'-O-)-methyltransferase
RsmI is an S-AdoMet (S-adenosyl-L-methionine or SAM)-dependent methyltransferase responsible for the 2'-O-methylation of cytidine 1402 (C1402) at the P site of bacterial 16S rRNA. Another S-AdoMet-dependent methyltransferase, RsmH (not included in this family), is responsible for N4-methylation at C1402. These methylation reactions may occur at a late step during 30S assembly in the cell. The dimethyl modification is believed to be conserved in bacteria, may play a role in fine-tuning the shape and functions of the P-site to increase the translation fidelity, and has been shown for Staphylococcus aureus, to contribute to virulence in host animals by conferring resistance to oxidative stress.
Links
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Statistics
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PSSM-Id: 381175
Aligned: 199 rows
Threshold Bit Score: 204.925
Created: 4-Feb-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:SAM binding site [chemical binding site]
Evidence:
  • Comment:SAM = S-adenosyl-L-methionine
  • Structure:5HW4; Escherichia coli K-12 ribosomal RNA small subunit methyltransferase I (RsmI), binds S-adenosylmethionine, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #                                                                         
3KWP_A     19 YLVPTPIGNLDDXTFRAVKTLTAVDLIAAEDTRNTQKLLNHf-----eITTKQISFheHNTQERIPQLIAKLkqgXQIAQ 93  Lactobacillus brev...
5HW4_A      5 YIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHf-----gINARLFALhdHNEQQKAETLLAKLqegQNIAL 79  Escherichia coli K-12
OGM34107   20 YIVATPIGNLEDITLRAIRVMGEVDIILAEDTRVAKKLLSSlnakwqiKNAKLVSYhqHSGEGKKLEILNYLldgKNIAL 99  Candidatus Woeseba...
B0SPB4     11 YVVATPIGNMGDITLRAIDVLKEVDLVLCESAKETKSLFHKl-----gISTPVLALykDHSETPFANVLEQLkqgKSMAL 85  Leptospira biflexa...
P47302      5 KVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLlni--dcKQKKFVINnsFKEKQNLTFAEEFIt-nFKCCL 81  Mycoplasma genital...
NP_604101   3 YIVATPIGNLEDMTFRAIRTLKEVDYIFAEDTRVTKKLLDHy-----eIKSTVYRYdeHTKQHQIANIINLLkeeKNIAL 77  Fusobacterium nucl...
NP_713174  18 YVVSTPIGNLEDLTFRALRILKNTDRILCENSGHSKKLFRSy-----sIHTQCSTLykDQSENPYLSILEELkegKTFAL 92  Leptospira interro...
Q661J3      3 YIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRy-----kINKKMISCnaVTENKKIRLLLDYLakgDSVAF 77  Borreliella bavari...
NP_970816   5 FVVATPIGNLKDISFRALETFQEADFIACEDTRHTLGLLTHy-----eISKPLISCraVNEAAASEKIVKLLdegKKIAY 79  Treponema denticol...
PID78105   16 YIVATPIGNIEDITLRALRILKEVDLVAAEDTRNTQKLFSMl-----dINTKLTSLheHNEKEKSAYLVELVlsgKKIAV 90  Deltaproteobacteri...

Feature 1        ###  ###                        ##                   #                       
3KWP_A     94 VSDAGXPSISDPGHELVNACi--DAHIPVVPLPGANAGLTALIASGLApQPFYFYGFLDRKPKDRKAEIAGlaqrpeTLI 171 Lactobacillus brev...
5HW4_A     80 VSDAGTPLINDPGYHLVRTCr--EAGIRVVPLPGPCAAITALSAAGLPsDRFCYEGFLPAKSKGRRDALKAieaeprTLI 157 Escherichia coli K-12
OGM34107  100 VSDAGTPGISDPGNELIDFLlsyKPDLKIIPIPGASAITTALSVSGFRaDKFLFLGFWPKKKVKRLIELIRlt--kmSFV 177 Candidatus Woeseba...
B0SPB4     86 VSDAGTPGVSDPGSQMVRTAr--ENGISIVPVPGASALTALLSVSGFQvNPTYFLGFLSEKPSKKRRELERareiegLIV 163 Leptospira biflexa...
P47302     82 VSDAGYPSLSDPGNEMINWIiskNKEIRIEVINGPSALMCGLITSGFKtTPLLFLGFLSHKQNQLKNYLSTyqnqksTIV 161 Mycoplasma genital...
NP_604101  78 VTDAGTPCISDPGYEVVDEAh--KNNIKVVAIPGTSALTASASIAGISmRRFCFEGFLPKKKGRQTLLKQLaeekerTIV 155 Fusobacterium nucl...
NP_713174  93 VSDAGTPGISDPGSHLIRVVr--EKGFKISPIPGPSALTALLGISGWQaNPFLFLGFLSEKKGKKRNQLYEwkefegLIM 170 Leptospira interro...
Q661J3     78 VSDAGTPGLSDPGSLLVAAAf--REGYKVCPIPGVSSFNTIVSVNPFRdKSVLFEGFLPNKGLKRFKKIAElykrgdAFV 155 Borreliella bavari...
NP_970816  80 ASDAGTPAISDPGSILVRTAr--KAGHTIIPIPGASAFGAIMSIAGTYdKTVVFEGFLSPKAGKRKRRLQElfdfgaGFV 157 Treponema denticol...
PID78105   91 VSDAGTPLVSDPGYRFIEEGl--KKGIRFVPVPGVSAVTALASVSGFFaGKFAFFGFFPSKESLRNKVLDElensdyPIM 168 Deltaproteobacteri...

Feature 1      ##                        # ## #                          ###    
3KWP_A    172 FYEAPHRLKKTLQNLAagfgDERPAVLCRELTKRYEEFLRGSLAELAnwaa--tdtvRGEFVVLVG 235 Lactobacillus brevis ATCC 367
5HW4_A    158 FYESTHRLLDSLEDIVavlgESRYVVLARELTKTWETIHGAPVGELLawvkedenrrKGEMVLIVE 223 Escherichia coli K-12
OGM34107  178 FYESPFRILRTLETLEkafgSDLRVFVGRELTKMHETVYRGRIRKVKekls--kekiKGEMVVVVE 241 Candidatus Woesebacteria bacteri...
B0SPB4    164 FYESVHKLPRLYPILEel-fPETEVLMGRELTKAFEEVVYYANPRELannp---pnaKGEFVFLLN 225 Leptospira biflexa serovar Patoc...
P47302    162 FFEAVHRLENTLETVKnv-fKNNDVFIGRELTKLHESHYWFNTSENTlpd----itlKGEFVIVID 222 Mycoplasma genitalium G37
NP_604101 156 IYESPFRIEKTLKDIEtf-mGKRDVVIVREITKIYEEVLRGSTSELIekle--knpiKGEIVLLIE 218 Fusobacterium nucleatum subsp. n...
NP_713174 171 IFESVHRIEDTLNSIQev-fSDCEFLIGREMTKIHEEILHFSPFATEkske---fasKGEFVVLIN 232 Leptospira interrogans serovar L...
Q661J3    156 LLESGHRLLKLLVEISsv-sLDAKILIGREMTKIYEEYQIGKPLELKkyfesskekvKGEFTILVS 220 Borreliella bavariensis PBi
NP_970816 158 LYESPYRIVKLLADIAei-dSNRELIIGRELTKLHEEIIKGPASEVLqnfek-rpsiKGEFSVFVT 221 Treponema denticola ATCC 35405
PID78105  169 FFESPKRIVKTLGEIIek-kGDRKGVLGRELTKIYEEVIRGNLSEIIeille-kekiKGEICFFVE 232 Deltaproteobacteria bacterium

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