5Y81,3QB0,5I9E,5NBL,8A5O


Conserved Protein Domain Family
ASKHA_NBD_Arp4_ACTL6-like

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cd13395: ASKHA_NBD_Arp4_ACTL6-like 
nucleotide-binding domain (NBD) of the actin-related protein 4 (Arp4)-like subfamily
The Arp4-like subfamily includes Arp4, also called actin-like protein 4, from fungi and plants. Saccharomyces cerevisiae Arp4 acts synergistically with Arp8 to depolymerize F-actin; it binds ATP, but unlike conventional actin, does not form filaments. It is a component of the NuA4 histone acetyltransferase complex, the chromatin-remodeling INO80 complex and the SWR1 chromatin remodeling complex. Arabidopsis thaliana Arp4 is involved in several developmental processes including organization of plant organs, flowering time, anther development, flower senescence and fertility, probably by regulating the chromatin structure. This family also includes human homologs of yeast and plant, which are actin-like protein 6A (encoded by the ACTL6A gene; also known as ArpNbeta, 53 kDa BRG1-associated factor A/BRG1-associated factor 53A/BAF35A, and INO80 complex subunit K/INO80K) and actin-like protein 6B (encoded by the ACTL6B gene; also known as ArpNalpha, 53 kDa BRG1-associated factor B/BRG1-associated factor 53B/BAF35B). ACTL6A and ACTL6B are involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). They are components of numerous complexes with chromatin remodeling and histone acetyltransferase activity. ACTL6A is also a putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Schizosaccharomyces pombe actin-related protein 42 (Arp42) is also included in this family. It is also a component of SWI/SNF and RSC complexes.
Statistics
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PSSM-Id: 466846
Aligned: 64 rows
Threshold Bit Score: 436.612
Created: 1-Nov-2012
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Structure:3QB0; Saccharomyces cerevisiae Arp4 binds CaATP, contacts at 4A
  • Structure:5I9E; Saccharomyces cerevisiae Arp4 binds MgATP, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    ####                                                                 
5Y81_F         12 GDEVSAVVIDPGSYTTNIGYSGSDFPQSILPSVYGKYTadegnkkif------------------------------seq 61   Saccharomyces...
3QB0_A         20 GDEVSAVVIDPGSYTTNIGYSGSDFPQSILPSVYGKYTadegnkkif------------------------------seq 69   baker's yeast
XP_002679390    6 GDEVGAVVIDVGTHTIKAGFAGEENPKAIIPSVIGTVFdsddkskissyy------------------------vgnkei 61   
EWM21911        4 GDEVFSVVGDLGTQSSRFGYAGEDLPKVVFPSAIGNVVhrtgseedgdrkkkstggassdvegavvaktkantsgtlgea 83   Nannochlorops...
XP_009492900   19 GDDTPAVVLEIGSYATRAGWAGDLSPEANILSSISVNTsdparasrf-----------------------------vgdh 69   Fonticula alba
XP_013756800    4 GDEVAALVLDIGSSTTRIGFAGEDTPKSVMPSCVGARVepakasgavgssasaneaamdle-dpvhhsgagksryivgsg 82   Thecamonas tr...
OAO16539        4 SDETGGIVVDIGSSSIKLGYGGEDVPKAVYPSSYGFNSqdpptyyl-------------------------------gtq 52   Blastocystis ...
GAX25025        4 GDETGSFVGDVGSHVARFGYGGDDAPRYVVSSRGVEASgkvlsscykspvte-------------------irtvmqpps 64   Fistulifera s...
GBG25981        4 GDETAALVGDLGSRTFKFGFAGEDSPKGWIVSATGSATgtaaamdvegegaes------------------kprslvgds 65   Hondaea ferme...
PXF43717       10 SDDIRAIVADCGTYHIRIGSSGDDTPRAIIPSAVGLEHtkadvemldvnknegkes-----------dkkqnsyvagdvl 78   Gracilariopsi...
Feature 1                          #                                                              
5Y81_F         62 sigipRKDYELKPIIENGLVIDWDTAQEQWQWALQNelyln-------------------------------snsgIPAL 110  Saccharomyces...
3QB0_A         70 sigipRKDYELKPIIENGLVIDWDTAQEQWQWALQNelyln-------------------------------snsgIPAL 118  baker's yeast
XP_002679390   62 sfarpGMEVQNIFNSDTGEIQNIDYFEKALNYIYDErlgv--------------------------------kpneHPLL 109  
EWM21911       84 nigirRTDLELSFPLINGHVQDWDAVECIWAHAFEQlsvke-------------------------------gakgVPYM 132  Nannochlorops...
XP_009492900   70 avftrRDDAELIQPIRAGAVTNWDDAEAIWSQAFNQlgav---------------------------------kgeDPLF 116  Fonticula alba
XP_013756800   83 alcvsRPHVELTPIMHHGLITDWEGTQAILNHAFTSvlqv--------------------------------dpsqHPLL 130  Thecamonas tr...
OAO16539       53 dlntrRDQMDVFSSFMNGKISNYDAIEKLLDYGITAglry--------------------------------kaseHPLL 100  Blastocystis ...
GAX25025       65 mkddaPLIDPVAYLSQGECVYDWDAYQSVWEQALERmmvrhatkhrsrpvslktkntetkseamnddsctldqpvvHPLL 144  Fistulifera s...
GBG25981       66 alqhaCEDVDVTWARKDGKVHSWDAVEDLWTHAVSYglns--------------------------------dareHPIL 113  Hondaea ferme...
PXF43717       79 lnaprRICHVIPIHDFEKQQINWDAMQTCWESGLNSlqi----------------------------------kpdAPFL 124  Gracilariopsi...
Feature 1                                                                 # ####                  
5Y81_F        111 LTEPVWNs-------TENRKKSLEVLLEGMqfEACYLAPTSTCVSFAAGRPNCLVVDIGHDTCSVSPIVDGMTLsk-sTR 182  Saccharomyces...
3QB0_A        119 LTEPVWNs-------TENRKKSLEVLLEGMqfEACYLAPTSTCVSFAAGRPNCLVVDIGHDTCSVSPIVDGMTLsk-sTR 190  baker's yeast
XP_002679390  110 MSEDPLCs-------SSGREKLASLVFEKCniPSFFLSKQPVLSCFANGKYTALVVDSGHNATTVTSVHDGLALrqstIK 182  
EWM21911      133 YALPAWAs-------RAESQKSAELAFERFdlGALYLARSPMLSAFSTGRSSGVVCDCGAGSTSAVPILDGYVLnq-lVG 204  Nannochlorops...
XP_009492900  117 MIEPSFAp-------PSFRNTLAEHMFETYslPAFYVARDAVCAAFASHRATALVVDIGATKTTVTPVLDGIALrk-aIA 188  Fonticula alba
XP_013756800  131 FTESVFNt-------RSAREKLTELAFETYgaPAYFVAKDAVLSTYAVGKPSGVVLSSGGSVTTASVVSDGYVLqktvVR 203  Thecamonas tr...
OAO16539      101 IVEDPFSf-------AEQRMKLAELMFEKLgfPAIYFIKSSSCIGFSLGKTTCMSVDFGASGIRVVPVYDGYPLmt-aSA 172  Blastocystis ...
GAX25025      145 LVAPGCTdlaspsnqRKQIQQMTELCMETWqcPALFVAPTPMLAAFAMGRSTATVVDLGAGGCRVTPLVDGLVLkq-aQR 223  Fistulifera s...
GBG25981      114 AATPTYEp-------EADRAKLAEILLEKLesPAVFMPRSAILSAFSVGRATSLVVDIGAARSCACAVHDGFVVrk-tVR 185  Hondaea ferme...
PXF43717      125 IIEPARMw------sPENRAKALERAFEGLhvPAAFIAKGSAMSAFASARTSACVIDVGYYDSCAVPVVDGYAVqr-kIV 197  Gracilariopsi...
Feature 1              #                                                                          
5Y81_F        183 RNFIAGKFINHLIKKALepk----------------------------------eiiPLFAIKQRKPEFIKktfdy---- 224  Saccharomyces...
3QB0_A        191 RNFIAGKFINHLIKKALepk----------------------------------eiiPLFAIKQRKPEFIKktfdy---- 232  baker's yeast
XP_002679390  183 TTKVCGKVLDEDLRNLVktkrgg-----------------------------kdilaPHQFTKQRSSQQPSskstkive- 232  
EWM21911      205 RSERGGDWLDGKVGEWLesrk---------------------------------akiGLFGFERGTIPEYMp-------- 243  Nannochlorops...
XP_009492900  189 WQPLGGDLLDALALHAVakrevafgvapgedqaaasaattgspqrrtlrpgpnhtitPGYLVLDKRPTNAGqpaqfkt-- 266  Fonticula alba
XP_013756800  204 NHAIGGNSLNKVLHHLVeaag--------------------------------grvtPRHGFKLVTDDEGNttveyt--- 248  Thecamonas tr...
OAO16539      173 VSPYGGDYLNSLLFDKLssqk--------------------------------iqvtPHCFLKKQIVEGVVaniqri--- 217  Blastocystis ...
GAX25025      224 RNGRGGDWLDHVLWHAAlsqq--------------------------------atlrPRYQIRQTTSSANNn-------- 263  Fistulifera s...
GBG25981      186 TSKVAGDFIDEHLLRLLetsvlphaqqd-------------------vanddsqaqnPSSHSSSSTIVVPKsreel---- 242  Hondaea ferme...
PXF43717      198 KSVVGGKYISDMLYDWAnnildgrlnhdgtehr--------tkrlrddetkkvdwirAHHEIKKERLDGVGtkrefrvtd 269  Gracilariopsi...
Feature 1                                #  ##                                                    
5Y81_F        225 -------evdkSLYDYANNRGFFQECKETLChicpt-------------------------------------------- 253  Saccharomyces...
3QB0_A        233 -------evdkSLYDYANNRGFFQECKETLChicpt-------------------------------------------- 261  baker's yeast
XP_002679390  233 ----hhidgltQSFNDYSINRIVGDMKINLLras---------------------------------------------- 262  
EWM21911      244 -----------TTYWRFQEQRVLRDVKESCClipqvd------------------------------------------- 269  Nannochlorops...
XP_009492900  267 ----rsfpnthPSFHSFHQLRVAADWRQSITavppsplpsfsdt-------------------------------saagc 311  Fonticula alba
XP_013756800  249 -----ddirvtHSYDSFTRSALLDDIKRSVCafap--------------------------------------------- 278  Thecamonas tr...
OAO16539      218 -----dfpntrASFLKYAIMDIVRDMKECCFhla---------------------------------------------- 246  Blastocystis ...
GAX25025      264 -----------SAFHRWAMQDLFQEFRTSEYtwplsltp----------------------------------------- 291  Fistulifera s...
GBG25981      243 -------srikPSFVRMQQLPILTDLKENVCrvwt--------------------------------------------- 270  Hondaea ferme...
PXF43717      270 vsnqgalaqatEYQRQFYRLRIMDDMKVSTFrvsqtrmgengttkesdkgsnndgkaktnkdgaardgngkeddgknske 349  Gracilariopsi...
Feature 1                                                                                         
5Y81_F        254 ---ktleeTKTELSSTAKRSIESPw-NEEIVFDNETRYGFAEELflpkeddipanwprsnsgvvktwrndyvplkrtkps 329  Saccharomyces...
3QB0_A        262 ---ktleeTKTELSSTAKRSIESPw-NEEIVFDNETRYGFAEELflpkeddipanwprsnsgvvktwrndyvplkrtkps 337  baker's yeast
XP_002679390  263 ------edGAVQTGNTPLVSFELNd-GNDIEIGLERFSICERLFnh---------------------------------- 301  
EWM21911      270 --sddgavFDIEENDAEKGAYELPd-GTRVKADKTLCRIPELLMvrgdgagcsisp------------------------ 322  Nannochlorops...
XP_009492900  312 aaraqahtSLSVSAHGSQRLYEFPc-GFNDLYGGLERAVCGEVLfnprdilqayglpisypta----------------- 373  Fonticula alba
XP_013756800  279 -----adaFGASTGSVPVVPYELPd-RTVLDLGPERFLVPEVLFrprwfyedrfrphatasepvamdtgedsvaaasrlp 352  Thecamonas tr...
OAO16539      247 -----skpIKQSPPPTTPISYTLPd-NQEVHLDQDAYYIPEVIMtgngt------------------------------- 289  Blastocystis ...
GAX25025      292 -ptnvpfsSPSLEDTTTPLGYQLPd-GTTLNVSQDMCQVHELLFsdstpfiesss------------------------- 344  Fistulifera s...
GBG25981      271 ----evplDESKAAKYAAVEYELPd-GTIVSLGAERFRSPELLMrphldvaylkek------------------------ 321  Hondaea ferme...
PXF43717      350 gkegkeskSKDKGTTERSTEYCLPdgNILNLNDNDGLAIPDMLFrtdea------------------------------- 398  Gracilariopsi...
Feature 1                                                                                         
5Y81_F        330 gvnksdkkvtpteekeqeavskstspaansadtpnetgkrpleeekppkenneliGLADLVYSSIMSs-DVDLRATLAHN 408  Saccharomyces...
3QB0_A        338 gvnksdkkvtpteekeqeavskstspaansadtpnetgkrpleeekppkenneliGLADLVYSSIMSs-DVDLRATLAHN 416  baker's yeast
XP_002679390  302 -----------------------------------------------------eyGLAKLTCDSINKc-DVDLRKELYSN 327  
EWM21911      323 -------------------------------------------advspsregafhDLPSMVYTAVEKa-DVDVRKELLGN 358  Nannochlorops...
XP_009492900  374 -------------------------------------glppsvshtpgaessnylGLPELIERCLANv-DVDNRAALASH 415  Fonticula alba
XP_013756800  353 aap--------------pltpddsirkpewlskpgtryndadpisgdlghrddslGVHEMVYKSVSLa-DVDLRRDLFGS 417  Thecamonas tr...
OAO16539      290 ---------------------------------------------------degpPLPNTIYSCIQNc-DVEIRKELTSN 317  Blastocystis ...
GAX25025      345 --------------------------------------------tmpphatlsnaPLHQLLHESLSAvgDVPMRKELASQ 380  Fistulifera s...
GBG25981      322 --------------------------------------------isaselekeptSVPEVLRDALQSs-HVDLRRELVQQ 356  Hondaea ferme...
PXF43717      399 ---------------------------------------------------kgmlSISEMAFKAVSGc-DVDLRRDLFNS 426  Gracilariopsi...
Feature 1             ### ##                                                           #          
5Y81_F        409 VVLTGGTsSIPGLSDRLMTELNKILPs-LKFRILTTGht---------------------------iERQYQSWLGGSIL 460  Saccharomyces...
3QB0_A        417 VVLTGGTsSIPGLSDRLMTELNKILPs-LKFRILTTGht---------------------------iERQYQSWLGGSIL 468  baker's yeast
XP_002679390  328 ILLVGGNtLIPGVPTRYEHELIRMAPnnVKVKHPLNTkisanihtlstsmgnsstqstmqtavaqlqERKYASFIGGSIL 407  
EWM21911      359 VILTGGGsCFRGSSERLQYELTEKVPvaYKVKIVTATp----------------------------lERRFSAWIGGSIL 410  Nannochlorops...
XP_009492900  416 IALAGGCsAIDGLASRLSAELGQRLQsfQQPRIIAEAsp---------------------------mDRRNMAWTGGSIV 468  Fonticula alba
XP_013756800  418 VIVTGGNtLFPDFTPRLRKHLAPLLPqtFKVKVSSINtp---------------------------tEQRFAPWIGGSIM 470  Thecamonas tr...
OAO16539      318 IVLAGGGcCMKGLSARLDSELSSRLNasFKPKLYSSEp----------------------------iYSPFNAWTGGSII 369  Blastocystis ...
GAX25025      381 ILLVGGMaGVSQLAPALSSRLRWPSA--FTPKIHCSPyl---------------------------eERTSAGWIGGSIL 431  Fistulifera s...
GBG25981      357 IMLVGGGaLVPGTLERLTKELGVCLPsaLKPRFLSPGk----------------------------yERLFSTFTGGSIL 408  Hondaea ferme...
PXF43717      427 VVITGGTsLMPGLVERVTRELALMSPqaYKLKVYAPSny---------------------------vERTCASWIGGSIV 479  Gracilariopsi...
Feature 1                             
5Y81_F        461 TSLGTFHQLWVGKKEYEEVG 480  Saccharomyces cerevisiae S288C
3QB0_A        469 TSLGTFHQLWVGKKEYEEVG 488  baker's yeast
XP_002679390  408 ASLGSFQSMWISKSEFDEMG 427  
EWM21911      411 ASLGSFQQLWLSKAEYEEMG 430  Nannochloropsis gaditana
XP_009492900  469 TSLSSFGTKWVSRAEWEESG 488  Fonticula alba
XP_013756800  471 ASLGSFHSLWISKQEYEEHG 490  Thecamonas trahens ATCC 50062
OAO16539      370 SSLGSFQQMWISKEEYKEKG 389  Blastocystis sp. ATCC 50177/Nand II
GAX25025      432 TSLGSFQQLWLSQAEYEEYG 451  Fistulifera solaris
GBG25981      409 ASLGSFQQLWISKAEYEEHG 428  Hondaea fermentalgiana
PXF43717      480 SSLGTFQQAWISRAEYDELG 499  Gracilariopsis chorda

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