1KHG


Conserved Protein Domain Family
PEPCK_GTP

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cd00819: PEPCK_GTP 
Click on image for an interactive view with Cn3D
Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate to yield phosphoenolpyruvate and carbon dioxide, using a nucleotide molecule (GTP) for the phosphoryl transfer, and has a strict requirement for divalent metal ions for activity. PEPCK's separate into two phylogenetic groups based on their nucleotide substrate specificity, this model describes the GTP-dependent group.
Statistics
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PSSM-Id: 238417
Aligned: 32 rows
Threshold Bit Score: 714.788
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1KHG_A; Human PEPCK binds divalent cation

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                     #                 
1KHG_A       30 VREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEeGILRRLkky--dnCWLALTDPRDVARIESKTVIVTqeqrdtvp 107 human
AAD24794     28 AQRFVGKWVEICKPTGVYICDGSKKERQELTQKLVElGSLHKLppy--enNYITCTDPRDVARVESKTWICSehehdtvp 105 Schistosoma mansoni
P20007       52 VRAFVEEGIALCQPDQVHICDGSEQENKVLIKSLLEaGTIVPLpky--dnCWLARTNPADVARVESRTFICTerreetip 129 fruit fly
Q05893       42 VQRFVAEKAELMKPSAIFICDGSQNEADELIARCVErGVLVPLkay--knNYLCRTDPRDVARVESKTWMITpekydsvc 119 pig roundworm
Q9HLV2       13 AMKWIEGIKKFTEAEDVVVCDGTPEEFKQISNELIKsGEFIKLnenrypnSFLYRSDRTDVARSEERTFIAApdas---- 88  Thermoplasma aci...
ZP_00000135  26 LNGRLETFVRMLEPANIVVITDPDFYEKKLVGEMATdHELIELnrkyysnSYLYRSNPDDVARTEKDTYISSldek---- 101 Ferroplasma acid...
AAG47713     23 FNELLLDAIELMRPSSLYIQTSSKEEIDYARSNALAqGEEHQLam---pgHTYHFDSPDDQGRDKKNTLILLddqt---- 95  Giardia intestin...
O58050       24 LHEFLAEWIEWLEPSKVFVCTDSPEDEEYVRWKALYyGEEKMLei---pkHTVHYDNYYDQARDKTNTKLLVpkgv---- 96  Pyrococcus horik...
Q97VS5       28 LIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKKALEsKEEIRLqt---sgHTIHFDHPLDQARAREDTFILSdtk----- 99  Sulfolobus solfa...
NP_497134    44 VQRFVAEKAELMNPAGIYICDGSQKEYDDIVDKLVErGVLTPLkay--enNYLCRTDPRDVARVESKTWMVTkdkydsvc 121 nematode
Feature 1                                                                                       
1KHG_A      108 -ipktglsqLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGt---PV 183 human
AAD24794    106 nvprgvkgvLGRWISPGDLDVEIKDRYPGCMEGRVLYVIPFSMGPIGSPLSKVGIEITDSIYVVLCMIIMTRMHpdvwKV 185 Schistosoma mansoni
P20007      130 tpvegvkgtLGNWISPSDMDAAVQQRFPGCMKGRTMYVVPFSMGPVGSPLSKIGIELTDSAYVVASMRIMTRMGaavlRQ 209 fruit fly
Q05893      120 htpegvkpmMGQWMSPDEFGKELDDRFPGCMAGRTMYVIPYSMGPVGGPLSKIGIELTDSDYVVLCMRIMTRMGepvlKA 199 pig roundworm
Q9HLV2       89 -----magsLNNHMTLQQVSEVWNKFFRGAYRGKTMFVIPYALGPLNSRFTDYGIEITDSRYVVLNLHYITRMGk---QV 160 Thermoplasma aci...
ZP_00000135 102 -----nagaTNNWMEPEHLKSRIFNLIKGSMKNKTMYIVPFILGPAGSKYSEAGIQITDNPYVVINLIKISLVGk--eAI 174 Ferroplasma acid...
AAG47713     96 -----gldaDVMTRDRKEGTAHVREIMKGCMEGKQMLIAFYCLGPVNCSFSKTAVQFTDSWYILHSENVLYRNGfa-qMV 169 Giardia intestin...
O58050       97 ------elpFLNTMNREEGLKEIREIMKGIMRGKELFICFFVLGPTNSIFTIPAVQLTDSAYVAHSEFLLYRKGyeefKR 170 Pyrococcus horik...
Q97VS5      100 -------ipYVNTKPRNEGLSEMLTLLKGSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSENILYRIAf---ED 169 Sulfolobus solfa...
NP_497134   122 htpdgvrpiMGQWMSEEQFGVELDSRFPGCMAGRPMYVVPYSMGPIGGPLSKNGIELTDSPYVVLCMRTMTRMGt---KV 198 nematode
Feature 1                                                                       # #     ##      
1KHG_A      184 LEALgd----geFVKCLHSVGCplplqkplvnnwpcnpeLTLIAHLP------DRREIISFGSGYGGNSLLGKKCFaLRM 253 human
AAD24794    186 IESKk------eFVRCVHSVGCptsshreiinhwpcnpeKTIISHVP------KERLIMSFGSGYGGNSLLGKKCFaLRI 253 Schistosoma mansoni
P20007      210 LAKKe------eFVRALHSVGApangqveq-pswpcdpeRTIILHKP------AENLIVSYGSGYGGNSLLGKKCFaLRI 276 fruit fly
Q05893      200 LAKNng-----eFVRCVHSVGQpkpvatkvinhwpcnpeKTIIAHRP------AEREIWSFGSGYGGNSLLGKKCFaLRI 268 pig roundworm
Q9HLV2      161 IGSMpe-----kFVKGVHATGTld-------------pgNKFIIHIPwdkpegVDADILSVNTNYGGNALLSKKCHaLRI 222 Thermoplasma aci...
ZP_00000135 175 NRIEnt----gkYVVAIHVTGTld-------------knNRYIAHFT------DEDLIISVNTAYGGNALLTKKGYaLRI 231 Ferroplasma acid...
AAG47713    170 NRHMketsanrsFYAFYHSVGRtdsanr------pvdidKRRIYINP------VEHLVYTINNSYAGNALACKKLA-LRL 236 Giardia intestin...
O58050      171 LGPTk------nFFKFVHSAGElderk-------tsknlDKRRIYIDl-----LDETVYSANTQYGGNTIGLKKLA-FRL 231 Pyrococcus horik...
Q97VS5      170 FSNNt------kFLRFVHSKGEpd--------------iKKRRIMIDl-----ANNTVYSVNTTYAGNSVGLKKLA-LRL 223 Sulfolobus solfa...
NP_497134   199 LEALgd----ndFVRCIHSVGLprpvkqkvinhwpcnpeKVMIAHRP------KEREIWSFGSGYGGNSILGKKCFaLRI 268 nematode
Feature 1                       #                       #######                     ##          
1KHG_A      254 ASRLAKEE---GWLAEHMLVLGITNPEGek--kyLAAAFPSACGKTNLAMmnpsl----pgwkVECVGDDIAWMKFDAq- 323 human
AAD24794    254 AGCIARDE---GWLAEHMLIMSVTNPKGee--kfIAAAFPSACGKTNMAMlepsl----pgwkVQCVCDDIAWMRFDEh- 323 Schistosoma mansoni
P20007      277 GSTIAKQE---GWLAEHMLILGITDPKGek--kyITAAFPSACGKTNLAMlnpsl----anykVECVGDDIAWMKFDSq- 346 fruit fly
Q05893      269 AMNIGYDE---GWMAEHMLIMGVTSPKGee--rfVAAAFPSACGKTNLAMlepti----pgwkVRVIGDDIAWMKFGAd- 338 pig roundworm
Q9HLV2      223 ASVRARKE---GWLAEHMLLLEVEDPHGrk--vyITGAFPSASGKTNLAMinppkqyaeagwkTRLLSDDIAWMKMKD-- 295 Thermoplasma aci...
ZP_00000135 232 ASVHARDN---SRMAEHMMALEVTSPSGrk--ygISGAFPSASGKTNLSMirtptd--magwdAQLLSDDIIWMHINN-- 302 Ferroplasma acid...
AAG47713    237 AIYDSNHHpekNNLTEHMFLSTFANPCIpndklnVCGAFPSACGKTSTSMap----------gSSIIGDDMVYMQIVDdn 306 Giardia intestin...
O58050      232 TIQRAVKE---GWLSEHMFLMRVNGPNGrk--tyFTGAYPSMCGKTSTAMip----------wENIVGDDLVFIKNVD-- 294 Pyrococcus horik...
Q97VS5      224 TIMKAVEE---GWLSEHMAIVGFNGDKGih---yFTASFPSGSGKTSTSMi------------GNLISDDLAFIREFE-- 283 Sulfolobus solfa...
NP_497134   269 ACNIGRDE---GWLAEHMLIMGVTNPEGee--kfIAAAFPSACGKTNLAMltptv----pgwkVRVVGDDIAWMKFGAd- 338 nematode
Feature 1                      # #        #                                                     
1KHG_A      324 ---GHLRAINPENGFFGVAPGTSVKTNPNAIKTIq--kNTIFTNVAETsDGGVYWEGideplasgvtitsw--------- 389 human
AAD24794    324 ---GVLRAINPEAGFFGVAPGTNVKTNPNAMATCm--kNTIFTNVAQTkDGHIFWEGledqyspdteiitwl-------- 390 Schistosoma mansoni
P20007      347 ---GVLRAINPENGFFGVAPGTSMETNPIAMNTVf--kNTIFTNVASTsDGGVFWEGmesslapnvqitdw--------- 412 fruit fly
Q05893      339 ---GRLYAINPEYGFFGVAPGTSHKTNPMAMASFq--eNTIFTNVAETaDGEYFWEGlehevknpkvdminw-------- 405 pig roundworm
Q9HLV2      296 ---GMLYATNPENGFFAVVPGTNYRTNKNAMITLs--rNTIFTNTGMTkTGEPWWEGldplqeelydwk----------- 359 Thermoplasma aci...
ZP_00000135 303 ---DSLYAINPEYGFFGVAPGTNATTNPNAMKAFn--rDTIFTNTALTtDGKPWWYSldpsvsevydwh----------- 366 Ferroplasma acid...
AAG47713    307 mgiRRCKAVNIETGMFGIISGVNPSDDPLIYDCLtserECIFSNVLVGeDGRPYWDGmyygkskndmvnepgllp--ssg 384 Giardia intestin...
O58050      295 ---GIARAVNVEIGVFGIIEGVNQKDDPIIWKVLhspvEIIFSNVLVK-DGKPYWNGmgieipdegenhsgkw------- 363 Pyrococcus horik...
Q97VS5      284 ---GLPKAVNPEIGVFGIIQGINARDDPIIWEVLhkpgEVIFSNVLMTeDGDVYWEGselpkpergynhe---------- 350 Sulfolobus solfa...
NP_497134   339 ---GRLYAINPEAGFFGVAPGTSHKTNAMAMESCr--aNTIFTNVAETaDGEYFWEGlekelkeakgytdeqlkhleitn 413 nematode
Feature 1                          #                                                            
1KHG_A      390 -knkewssedgepcaHPNSRFCTPASQCPIIDAAWe-sPEGVPIEGIIFGGRRPa-GVPLVYEAl-sWQHGVFVGAAMRS 465 human
AAD24794    391 gdhvklsdkskdpkaHPNSRFCCPASQCPIIHPKWe-dPHGVPISAIIFGGRRPe-GIPLVLQSf-dWKHGVMLGAALKS 467 Schistosoma mansoni
P20007      413 -lgkpwtkdsgkpaaHPNSRFCTPAAQCPIIDEAWe-dPAGVPISAMLFGGRRPa-GVPLIYEAr-dWTHGVFIGAAMRS 488 fruit fly
Q05893      406 -lgepwhigdeskaaHPNSRFTAPAGQCPIIHPDWe-kPEGVPIDAIIFGGRRPe-GVPLVFESr-sWVHGIFVGACVKS 481 pig roundworm
Q9HLV2      360 ---gvlrkpdgepiaHPNSRFTSPLSNYPFLSDRSe-dPEGVPVSAILFGGRRAs-LVPLVYEAf-nWNHGVFMGATMGV 433 Thermoplasma aci...
ZP_00000135 367 ----gnlttdlknaaHPNSRFTTPIKNYPYLSSKFy-dNEGLKIDAMLFGGRRSd-LIPLVRQAk-sWAQGVLFGAMIRA 439 Ferroplasma acid...
AAG47713    385 rnykgtwtkpelpimHGNARFTLNLKELRNVSPELd-nPDGVDVDLILYGGRDSn-TCPPVYQAh-nWAHGVYIGSSIES 461 Giardia intestin...
O58050      364 wrgkrdaegkeippsHKNARFTVRLEAFPNLDREAlnnPCGVEVGGMIFGGRDPd-TWPPVREAfdwEHGVITMGASLES 442 Pyrococcus horik...
Q97VS5      351 ---grwdresgkpasHPNARFTVPLTSFSNLDKNWd-nPNGVIIDGIIFGVRDYstLVPVVEAFs-wSHGVITIGASMES 425 Sulfolobus solfa...
NP_497134   414 wlgerwhigdegkaaHPNSRFTAPAKQCPNIHPDWe-aPQGVPIDAIVFGGRRPe-GVPLVFESf-sWEHGILVGALVKS 490 nematode
Feature 1                                                                       #  #  #         
1KHG_A      466 EATAAAeHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQhpaakLPKIFHVNWFRkdkegkFLWPGFGENSRVLEWMFN 545 human
AAD24794    468 EATAAAeFKGKQVMHDPMAMRPFVGYNFGHYLNHWLSMDKps-hkLPFIYHVNWFRlnkqgkYVWPGFGQNIRVIDWILR 546 Schistosoma mansoni
P20007      489 EATAAAeHKGKVIMHDPFAMRPFFGYNFGDYVAHWLSMEKrg--qVPKIFHVNWFRksaegkFMWPGYGENSRVLEWILR 566 fruit fly
Q05893      482 EATAAAeHTGKQVMHDPMAMRPFMGYNFGRYMRHWMKLGQpp-hkVPKIFHVNWFRqsadhkFLWPGYGDNIRVIDWILR 560 pig roundworm
Q9HLV2      434 EKTAASeGKVGELRRDPMAMRPFCGYNISDYFRHWIEMGRkl-srRPKIFYVNWFRrrqdgsFIWPGFSENFRVIEWILY 512 Thermoplasma aci...
ZP_00000135 440 ETTAATtGKVGILRNDPMAMIPFCGYNIGDYFQHWLDMGKlv-qhRPEIFYVNWFRkdsdgnFIWPGFSENFRVIEWIAS 518 Ferroplasma acid...
AAG47713    462 ETTAATlGTQGVVVSNPMANLDFLIVPVPMYLENHVRFGRllgsrAPKVFGVNYFLkdpttgEYLNSKLDKKVWVAWACG 541 Giardia intestin...
O58050      443 ETTAATlGKEGVRAFNPMSILDFLSVHIGDYIRNYLEFGRkl-kkTPKIFAVNYFLre--ngRWLNEKLDKAVWLKWMEL 519 Pyrococcus horik...
Q97VS5      426 ARTSAViGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKrl-rkSPKIFGFNYFLkd--dnKFLNSKEDKKVWVSWAVK 502 Sulfolobus solfa...
NP_497134   491 ETTAAAeFTGKNVMHDPMAMRPFMGYNYGKYLEHWIKLGKap-hkAPKIFHVNWFRetkdhkFLWPGFGDNIRVLDWILR 569 nematode
Feature 1                                                                                       
1KHG_A      546 RIDG--KASTKLTPIGYIPkedaLNLKGLGhinmme--lfsisKEFWDKEVEDIEKYLVDQvn--------adLPCEIER 613 human
AAD24794    547 RVNG--EDIGVNSPIGILPkkgsINFDGLDvdwde---nfslpKDYLLEDIDETMKYLHEQvg--------kdLPDVIQN 613 Schistosoma mansoni
P20007      567 RVNG--ESCYVDSAIGHIPaegaLNLDGMKdkvdvk-eifslpKEFWSQEVKDIRTYFESQvg--------adLPASIYQ 635 fruit fly
Q05893      561 RCSGd-ATIAEETPIGFIPkkgtINLEGLPnvnwde--lmsipKSYWLEDMVETKTFFENQvg--------sdLPPEIAK 629 pig roundworm
Q9HLV2      513 RLDH--NDNAIETPIGYIPe--nINTDGLNltkqdmeelfridRDGWREEMKSIGDYFSQIg----------nIPEDLLI 578 Thermoplasma aci...
ZP_00000135 519 RLDA--KANAIETPVGLIPdianFASGNLGknkleq--lfeidYPGWLKELNEIEPFFKKIgd---------sFPEELWR 585 Ferroplasma acid...
AAG47713    542 RTRG--HYRAKCTPIGWIPy--yEDLKSLFrseld---tdyteEQYNIQFTINVENYIQKMvrimkl-yggkqAPTEWVI 613 Giardia intestin...
O58050      520 RVHN--DVDAIETPIGYIPr--yEDLKVLFkqvln---kdysrEDYEKQFKIRVPELLAKIeriwkiyepidnIPEELFK 592 Pyrococcus horik...
Q97VS5      503 RVEE--TANAIYTPIGLIPf--yEDLKALFkrvlg---keygkEEYEKQFTIKLRKYLEKTeriieiylkfedIPSEVIN 575 Sulfolobus solfa...
NP_497134   570 RVAGgeEEIAIETAIGYVPkrgtINLDGLPridwnd--lmsipKDYWVEDVDESRHFLDTQvg--------sdLPQPIRD 639 nematode
Feature 1            
1KHG_A      614 EILAL 618 human
AAD24794    614 ELNNQ 618 Schistosoma mansoni
P20007      636 QLDEL 640 fruit fly
Q05893      630 ELEAQ 634 pig roundworm
Q9HLV2      579 EFEME 583 Thermoplasma acidophilum
ZP_00000135 586 EFHNM 590 Ferroplasma acidarmanus
AAG47713    614 MECRV 618 Giardia intestinalis
O58050      593 QLKDE 597 Pyrococcus horikoshii
Q97VS5      576 ELKMQ 580 Sulfolobus solfataricus
NP_497134   640 ELDKL 644 nematode

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