2X7M,3LMC


Conserved Protein Domain Family
Peptidase_M54

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cd11375: Peptidase_M54 
Click on image for an interactive view with Cn3D
Peptidase family M54, also called archaemetzincins or archaelysins
Peptidase M54 (archaemetzincin or archaelysin) is a zinc-dependent aminopeptidase that contains the consensus zinc-binding sequence HEXXHXXGXXH/D and a conserved Met residue at the active site, and is thus classified as a metzincin. Archaemetzincins, first identified in archaea, are also found in bacteria and eukaryotes, including two human members, archaemetzincin-1 and -2 (AMZ1 and AMZ2). AMZ1 is mainly found in the liver and heart while AMZ2 is primarily expressed in testis and heart; both have been reported to degrade synthetic substrates and peptides. The Peptidase M54 family contains an extended metzincin concensus sequence of HEXXHXXGX3CX4CXMX17CXXC such that a second zinc ion is bound to four cysteines, thus resembling a zinc finger. Phylogenetic analysis of this family reveals a complex evolutionary process involving a series of lateral gene transfer, gene loss and genetic duplication events.
Statistics
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PSSM-Id: 213029
Aligned: 102 rows
Threshold Bit Score: 95.8264
Created: 5-Apr-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding siteactive site
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1: Zn binding site [ion binding site], 7 residue positions
Conserved feature residue pattern:H H H C C C CClick to see conserved feature residue pattern help
Evidence:
  • Structure:2X7M: Methanopyrus kandleri Archaemetzincin (Amza) binds two Zn ions
  • Comment:Archaemetzincins bind two zinc ions, one is coordinated by histidines and is common to all metzincins, and a second one which is coordinated by 4 cysteine residues resembling a zinc-finger motif.
  • Comment:Archaemetzincins have an extended conserved sequence of HEXXHXXGX3CX4CXMX17CXXC

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
2X7M_A        22 KLCLVAFdgr------ipmLSSIVDRFEEHVSEYl------------gEVKVKKKrakl--------------------- 62  Methanopyrus ka...
XP_001630322  96 GPVMWCEsas------epsDGEDKPRSKRRKSSRia-----------sNMHILDH------------------------- 133 starlet sea ane...
EFW41870     134 KVTVLPMlpl-------rsWNDVEDEPKRKATTKgkgksg---slsplGVEVSGSlvr---------------------- 181 Capsaspora owcz...
XP_002670125  92 NIGLLWIekdg----dsndRRKYLTHLKRFVEAFyy----------giNVTILDSvhikkdkhghfves---ediiyrik 154 Naegleria grube...
XP_002673158 175 ALTIQTQdk---------sLNESVSPKQRKKKKRkk-----------eKVEKKKMylk---------------------- 212 Naegleria grube...
XP_001745194 110 IIYLQPIiderq---ptelEQLDLDFVSRFLEAFlp----------tmKVRVMEPwrievpakatktkahyettlkvghg 176 Monosiga brevic...
XP_003348550 196 RVRTRPCpdtvf--ggqlnLNDLLDGALHVLPDDayai------lllvDHDLYEDdeddf-------------------- 247 Sordaria macros...
CBF83223     218 KLYLRQLn-----------LDDLLDVAISILPEDayal------cmlvHHDLFEDdedif-------------------- 260 Aspergillus nid...
XP_003045852 148 NDYLAAFy-----------HPMTIQQSRMQLKWRpykgnppkegqtyiGLEVPTVrdf---------------------- 194 Nectria haemato...
EGZ13274       3 AVRIRARpssdglfggqlnLEDLLDTAIALLPADayal------llivDHDLYEEedidf-------------------- 56  Phytophthora sojae
Feature 1                                                                                        
2X7M_A        63 --pehAYSKv-------RGQYL----ARALLDTLRGmkg-------------eydRVLGLTSe---DLYAPg-------- 105 Methanopyrus ka...
XP_001630322 134 -----RWNTg-------RIQLK----VDGILKLLSRhrpp------------ntlCLIALTMs---DLYEEe-------p 175 starlet sea ane...
EFW41870     182 --vraRASPdg----vgVRQVN----LNDMIDGLLArmetl----------knayAILGVTSe---DLFEGn-------d 231 Capsaspora owcz...
XP_002670125 155 srrchEYDEdka--gedEIQFR----AADVIVPLNKlrkhl---------qdevsCVCGVTMd---DLYIGa-------k 209 Naegleria grube...
XP_002673158 213 --igdSYEAvtcrkaldHDQIQl--sISDVLDVLEMqvlp-----------ddafCMIALTLt---DLYMDe-------d 267 Naegleria grube...
XP_001745194 177 geysvAARRsrdg-pslHPQIG----LNDLADALLYkvpr------------dawCRVGVSMl---DLYEDd-------n 229 Monosiga brevic...
XP_003348550 248 -ccgrAYGGs-------RVCIVsssrYHPALDTLDYyivggidlahmwpashcqaFVDGVCSl--hDPPPPskkrkrtqk 317 Sordaria macros...
CBF83223     261 -vcgrAYGGs-------RVAVVstarYNPLLDDSLYvgrdha--------wpgshCVCYIEDic-kDHDPD--------- 314 Aspergillus nid...
XP_003045852 195 --fliRYRPgkd--gvsRMQVH----GPDLLDAVLNrmpp------------kahATVLLVDf---DLYDLsdd----en 247 Nectria haemato...
EGZ13274      57 -ccgrAYGGs-------RVAVVssarYSPALDTVQGveveh---------awpvsHCQAYVDtcvrNADSGggps-rkka 118 Phytophthora sojae
Feature 1                                                                                        
2X7M_A       106 LNFV---FGQARCp--gREAVVSVARLLDpd------------------------------------------------- 131 Methanopyrus ka...
XP_001630322 176 DLFV---AGMASGn--kGVAVFSFARYDPvitfstefwyei--------------------------------------- 211 starlet sea ane...
EFW41870     232 DLFT---MGRSYGg--sGVALISLARDNPalddvagdpaarewsfag--------------------------------- 273 Capsaspora owcz...
XP_002670125 210 DSFT---CGLAQGg--dSIGVFSMCRYDPlfrksapkkrnkkitvs---------------------------------- 250 Naegleria grube...
XP_002673158 268 DLFV---VGWAGN----FSALFSFARYDPnfthtlnggrtsassh----------------------------------- 305 Naegleria grube...
XP_001745194 230 DDFC---MGRAWGa--sRVAVLGFFRYAAelgkgr--------------------------------------------- 259 Monosiga brevic...
XP_003348550 318 KLEP---KAPADSvp-pGLDVPSTPMGAAlravqpls------------------------------------------- 350 Sordaria macros...
CBF83223     315 DSGTh--SGRKRKrkkpGIRQQPHNRSGPlesalaafist---------------------------------------- 352 Aspergillus nid...
XP_003045852 248 NNFT---VTKAYGg--sRIVLVSTFRLHPaldemqdidrehmwpashckvyaddliaqeeeiqttkrpkmsndepldsdk 322 Nectria haemato...
EGZ13274     119 KTAVrkgTTVAHLssamQAAVTASTSAPAsaq------------------------------------------------ 150 Phytophthora sojae
Feature 1                                                #   #     ##    #                  #  # 
2X7M_A       132 ---------------------------pELYLERVVKELTHELGHtFGLGHCpdrNCVMSFSSslLEVDRKspNFCRRCT 184 Methanopyrus ka...
XP_001630322 212 ----------------ektfnfkeeertSLMLQRSCKLLVHEIAHlFGIDHCiwyACCMNGSGhlGEDFSQpiHLCPVDL 275 starlet sea ane...
EFW41870     274 -----------pvsntpsatsspasntnSIWLYRACCTAVHELGHcLGMDHCiyfHCFMQGSAslLEARSQppYPCPLEL 342 Capsaspora owcz...
XP_002670125 251 ------------weedgiinedtltendLVILERCCKIVVHEIAHmVQVGHCvffDCIMNGSGhlEEDFRQsiHLCPVDL 318 Naegleria grube...
XP_002673158 306 -------------qqteennnlgvnqriDLILKRCLKTVVHETLHiFGLRHCifySCLMNGTAslHEDDETpmFMCPICE 372 Naegleria grube...
XP_001745194 260 -----------------------pprgvPALREAMVKTLTHEILHcFLFDHCtrgSCLMNTTGnlHEDFEAplWLCEECL 316 Monosiga brevic...
XP_003348550 351 ---------------------hsakdlhGLWFSRVARTVSHEIGHcFCLGHCsyyACVMQGTTsvAEDVRQppYLCPVCL 409 Sordaria macros...
CBF83223     353 ------------------ppqaggddlpALWRYRILRTVSHELAHcFGLDHCvyyACIMQGSAslAEDARQppYLCPVDL 414 Aspergillus nid...
XP_003045852 323 ppksisalpaaieaykeapepktideltGVWLARVAFAVSRGLGYcFGMEHCtyyACIMQGVSstRQQSHIppYLCPICT 402 Nectria haemato...
EGZ13274     151 -------------------------sdgALWLARVCRTASHELGHcFGMDHCvyyACSMQGSAglSEDARQppYLCPVDL 205 Phytophthora sojae
Feature 1             
2X7M_A       185 ELLQR 189 Methanopyrus kandleri AV19
XP_001630322 276 HKVQK 280 starlet sea anemone
EFW41870     343 VKMSA 347 Capsaspora owczarzaki ATCC 30864
XP_002670125 319 RKFQY 323 Naegleria gruberi strain NEG-M
XP_002673158 373 RKLQY 377 Naegleria gruberi strain NEG-M
XP_001745194 317 AKLCL 321 Monosiga brevicollis MX1
XP_003348550 410 AKVTH 414 Sordaria macrospora k-hell
CBF83223     415 AKVLA 419 Aspergillus nidulans FGSC A4
XP_003045852 403 TKLSW 407 Nectria haematococca mpVI 77-13-4
EGZ13274     206 AKLLH 210 Phytophthora sojae

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