Conserved Protein Domain Family
Diphthamide_syn

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pfam01866: Diphthamide_syn 
Putative diphthamide synthesis protein
Diphthamide_syn, diphthamide synthase, catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP. Swiss:Q16439 is a candidate tumour suppressor gene. DPH2 from yeast, which confers resistance to diphtheria toxin has been found to be involved in diphthamide synthesis. Diphtheria toxin inhibits eukaryotic protein synthesis by ADP-ribosylating diphthamide, a post-translationally modified histidine residue present in EF2. Diphthamide synthase is evolutionarily conserved in eukaryotes. Diphthamide is a post-translationally modified histidine residue found on archaeal and eukaryotic translation elongation factor 2 (eEF-2).
Statistics
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PSSM-Id: 460365
Aligned: 210 rows
Threshold Bit Score: 186.576
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
ERS98388      62 FPDAMLGDSPRVVKLLrEE--LTATaveiesgvaasekVYILADTSYSACCVDEVAAEHADADVVVHYGRSCLSPTS--R 137
XP_001022303  53 FPVENLSDAIKVQSSI-NKnlPATN-------------IYIISDHLKNSCCVDIVGSNHINYDIIFHFGVACLSESEeqQ 118
XP_007400287  60 FPDELLHLSVPIYRTLkAKagAGHD-------------FYVLADTSYGSCCVDEVAAQHVDADAMVHYGHACMSQTS--R 124
XP_003031304  59 FPDELLRYSVPIYRSLmSR--LGPGr-----------eLYVLADTSYGSCCVDEVAAKHADVDAVVHYGHACMSQTY--R 123
EGN97603      70 FPDLLLPDSVPIYRLLkRR--VGEAh-----------eLYVLADTSYGSCCVDEVAAQHVDADFMVHYGHACMSQTY--R 134
EMD35738      60 FPDELLRHSVPIFRRLkARlsPGRE-------------LYVLADTSYGSCCVDEVAAQHVDADAMVHYGHACMTKTS--R 124
EPS94679      58 FPDELLRDSVPIFRRLkAR--LGEGh-----------dLYVLADTSYGSCCVDEVAAQHVDAHAVVHYGHACMSQTS--R 122
XP_007849364  61 FPDELLHVSVSIYRKLkRK--VGSHr-----------eLYVLADTSYGSCCVDEVAAQHVDADAMIHYGYACMSQTY--R 125
XP_002992317   1 FPDNLLADSVAVTSALqSAcgSGVR-------------FFIMADTTFGSCCVDEVAAAHVNADCVVHYGPACLSPVT--N 65 
XP_007406103  57 FPDHLLPISVRIFKLLrSR--LPASnddk------aveIYLMADTTYGSCCVDCLAAQHVAAQAIVHYGPACLTTIA--N 126
ERS98388     138 LPVIYVFTQHELDEDAVVQSFAKELgapqeg---negddSE-GRQn---VVIMADVTYQDHVA-------P-VADRLRNL 202
XP_001022303 119 KKIKYIFPKTHMDLVGFTEQAFKLTk-------------SD-DGKvqnyIILIDQYQYYLSQE---------IFQQFRDQ 175
XP_007400287 125 LPVIYVFGKSLIGAKDCVARLSETLq-------------SL-ETAsl-qILLRTDVRYVHAAAesi--adK-LRAMLPST 186
XP_003031304 124 LPVIYVFGKKQIDVDDCVAKFVAQCrv-----------eDL-KRP----VVLRHDVGYTQQIP-------R-ILSKLRAQ 179
EGN97603     135 LPVLYIFGRRPIDVDDCATQLADTLksscsg---sdpleSN-KKP----VILRHDVAYTYQADti----vAkVREQLEAL 202
EMD35738     125 LPVIYVFGKKPVDSEDCASKLLQSLdas---------stSLeGKT----LVLRHDVAYAHRAD-------Q-ILEKLRAA 183
EPS94679     123 LPAIYVFGKKQVDPADCVDKFLQASgs-----------dIV-RNT----IILRLDVAYCHKAD-------A-ILERLRTA 178
XP_007849364 126 LPVFYVFGKKNLDIDDCVARLVDAFsass------eessPQ-AKT----IVLRHDVVYTHKASesi--syVeLRRFLNTT 192
XP_002992317  66 LPAYFVFLRTPLDINTCGEVVADLV--------------AK-DER----LLVLYDLEYAHAIP-------E-LRETVRQE 118
XP_007406103 127 LAVYYVLPRLPLDDSSILQVATELYsgfkaslerdkdteDGkELQ----LVLMYDVGYHWKVGksftlhsSfCCSQIK-- 200
ERS98388     203 g-------Y--KNV----LA-------TKvVH--RPLGSIP-NR----TIVGARDGae-vkDEDVDlheyAVFHIAQPPT 254
XP_001022303 176 -----------ENVtlalI--------QLdQA--QKINHLFyGYylnkSLDKSKKY--------------QLIFTGHEDS 220
XP_007400287 187 -----------SSLryhpVP-------RFtLP--ATAEPAI-RS----NSEDADGT-----EHQHD----TILYIGGESL 232
XP_003031304 180 --------L--DDL-------------EVlCE--ETPTRID-PS----SSSEPRHRt----AAENA----TILYVGGESL 221
EGN97603     203 g-------VsvPVLyq-tLP-------SRfSP--PAQKDAD-VT----TSTQLPIKgdlpvTLENG----VILYIGGESL 256
EMD35738     184 msiyapfeY--SPIplmsFP-------VSpNT--STQGETTvAT----NGHDTTSDsqlddAPRND----IILYVGGESL 244
EPS94679     179 l------sKptNIL----YS-------SIpTM--TCPSKQL-DG----PDQSDQNEpehnaPEEIDptdaVILYVGGESL 234
XP_007849364 193 p------gTllQHLqeifRPrgvqvmySQkQP--LNEAKRI-------SEPPLRDC--------------TIFYIGDENL 243
XP_002992317 119 apensgltF--AEI----IG-------RElNPckCDEEPIL-DT----DEHSIGGLkw--kGSTPT----KILWIGKEGP 174
XP_007406103 201 --------HldKHR----PN-------APlRP--NLNDPTV-DV----DSPVID-------TSSYD----AILYLGPCSR 243
ERS98388     255 SLLLALAARvKA-----FYIYATTD-TdnSAAgsLLAPRTMGLLGRRYAKVLTMATAGVIGILVNTLSVNNYLKAVDAIR 328
XP_001022303 221 DLLEKIFFAkDKfniekVYVYNNQI-K-------ESPPVSNKSLIKRLSMIEQARDIKCFGILIQNPNTQFCRAVTERVK 292
XP_007400287 233 GLTNLLLTHsSSn----VYSYDPKL-K--TTR--LESGRTNKLLMRRYAAVQKARDADVFGILVGTLGVASYLPLISRLR 303
XP_003031304 222 SLTNLLMTH-STc---dVYSYLPKS-R--EIR--LESGKTNRMLMRRYAAVQKARDADVFGILVGTLGVASYLPLIKHLR 292
EGN97603     257 GLTNLLMTH-ASc---dIYSYDPSS-K--TVR--QESTRTNRLLMRRYAIVQKARDADVFGILIGTLGVASYLPLISHIR 327
EMD35738     245 SLTNLLMTH-AHc---eVHTYDPKT-G--TAR--LESGRTNKLLMRRYVAVQKARDADVFGILVGTLGVAAYLPLISHLR 315
EPS94679     235 TLTNLLMTH-SSc---qVYSYDPHT-R--SSR--LESARTNRLLMRRYAVVQKARDADVFGILVGTLGVASYIPLISHLR 305
XP_007849364 244 GLTNLLMTH-SSc---nVISYSPSQ-R--TSQ--IESGRMNKMLMRRYAVVQKARDADVFGILVGTLGVASYLPLIKHIR 314
XP_002992317 175 ALTNVMLTYnSAa----TVRYDPASgEqtSIF--YSQYLIFNIEFSRYYLIERAKDANIVGIVVGTLGVAGYKQAIQNVK 248
XP_007406103 244 RLTHIMLTH-SSi---pIFALDPSS-S--PLAcqSQVPLAKKLLMKRYATIQRARDADVFGILIGTLGLKHDLELIHRTK 316
ERS98388     329 KRIADAG-KKSYLIVVGKLNPVKLANFAEIEGWVVVGCWESSLVedD-------AG-FYQPVVTPFELEMALMsd---dE 396
XP_001022303 293 QVIKYAN-KKFYTFYMSNLNEAKLNNFPEVEAFVIVSCYRNSLF--D-------LKkYYKLIVTPFELEMALR------N 356
XP_007400287 304 RLLARAQ-KKSYTITVGKLNPAKLGNFLEIECFVLVACPENSLV--E-------AKeFLRPIITPYELEVALQp-----E 368
XP_003031304 293 ETLKKAQ-KKSYTISVGKLNPAKLANFMEIECFVLVACPENSVI--E-------AKeFLRPIVTPFELQIALQa-----E 357
EGN97603     328 TLLARAR-KKSYTISVGKLNPAKLANFMEIECFVLVACPENSVI--E-------AKeFYRPIITPYELEVALQa-----E 392
EMD35738     316 KVLSRAH-KKSFTISVGKLNPAKLANFLEIECFVLVACPENSLI--E-------AKeFLRPIVTPYELEIALRa-----P 380
EPS94679     306 KMLAKAH-KKSYTISVGKLNPAKLANFLEIECFVLVACLENSVI--E-------AKdFLRPIVTPYELEVALRa-----E 370
XP_007849364 315 ALLAASR-KKSYTISVGKLNPAKLANFMEIECFVLVACPENSLI--D-------AKdFYRPIITPYELEIALQp-----E 379
XP_002992317 249 QMVKSAG-KKSYTLAVGKPNPAKLANFPECDVFVLIACPQTALV--D-------NKeFFAPLLTTFEAQLAFVr-----G 313
XP_007406103 317 SLIEKQFkKKSYIISVGKLKPEKLINFIEIECWVLIACPEHSLL--DegisvtrSKqFNVPVITPWELDLALKgsssirP 394
ERS98388     397 RIWGHKWW 404
XP_001022303 357 QSFSNYFL 364
XP_007400287 369 PLWTGQYV 376
XP_003031304 358 PDWTGRYV 365
EGN97603     393 GTWTGRYV 400
EMD35738     381 QSWTGRYV 388
EPS94679     371 QSWTGRYV 378
XP_007849364 380 PTWTGRYV 387
XP_002992317 314 KQWTGSYS 321
XP_007406103 395 RQWDGHYV 402
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