1LAM,1GYT,1LCP


Conserved Protein Domain Family
Peptidase_M17

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cd00433: Peptidase_M17 
Click on image for an interactive view with Cn3D
Cytosol aminopeptidase family, N-terminal and catalytic domains. Family M17 contains zinc- and manganese-dependent exopeptidases ( EC 3.4.11.1), including leucine aminopeptidase. They catalyze removal of amino acids from the N-terminus of a protein and play a key role in protein degradation and in the metabolism of biologically active peptides. They do not contain HEXXH motif (which is used as one of the signature patterns to group the peptidase families) in the metal-binding site. The two associated zinc ions and the active site are entirely enclosed within the C-terminal catalytic domain in leucine aminopeptidase. The enzyme is a hexamer, with the catalytic domains clustered around the three-fold axis, and the two trimers related to one another by a two-fold rotation. The N-terminal domain is structurally similar to the ADP-ribose binding Macro domain. This family includes proteins from bacteria, archaea, animals and plants.
Statistics
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PSSM-Id: 238247
Aligned: 195 rows
Threshold Bit Score: 317.566
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Substrate-bin..Zn-bindinginterface
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:Substrate-binding/catalytic site
Evidence:
  • Structure:1LCP_A bound to transition state analogue L-leucinephosphonic acid
  • Comment:catalytic mechanism is a two metal ion nucleophilic reaction with a bound water as the attacking hydroxide ion nucleophile
  • Citation:PMID 7619821
  • Citation:PMID 7578088

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1LAM          1 TKGLVLGIYskeke--------------edepQFTSAGENFNKlvs--------gkLREILNISgpplkaGKTRTFYglh 58  cow
1LCP_A        1 TKGLVLGIYskeke--------------edepQFTSAGENFNKlvs--------gkLREILNISgpplkaGKTRTFYglh 58  cow
NP_326206     1 MKGKKVIKVsn-------------------ekEFPISLKLIFKqe---------nmNDFLIQED------HQITEINs-- 44  Mycoplasma pulmo...
CAD84480     18 TSHLLIIFPktdki--------------pgkyQLPAEQVLNDLl------------QRQGIPVNr----lAETPLSAslq 67  Nitrosomonas eur...
AAR38210     18 KKNLHTFIL-----------------------FDEKHSNLFFAg-----------vLKANIKRQngkitdLKKTALSael 63  uncultured bacte...
YP_015871     5 INSKQNIKE-----------------------IELKFISKIDD-------------NKNLINTEd----lAISEFLDq-- 42  Mycoplasma mobil...
ZP_00565941  19 AASHILFVLp---------------------kRRPASLPFASL-------------LEARLRRAr-----SKYTDLAksh 59  Methylobacillus ...
ZP_00640847   5 NKALTAFQVpiidvttdeaifegegwdavvviATSLAEIKQDEv-----------sLLAAHASQidfrvgKSATLLFapg 73  Shewanella frigi...
YP_286161     2 IATLKTFPAlpet---------------aaqaGMSHVLVLLPAmqeipadcpgrasLMATLARRrl--dtDELSDAPvsa 64  Dechloromonas ar...
ZP_00837845  21 WDAVVLVTH-----------------------GLDAIAQEELAm------------LASHGAQVda--rvGRSTTLLfap 63  Shewanella sp. PV-4
Feature 1                                                                                       
1LAM         59 e-----dfpSVVVVGLGKktagideqenwheGKENIRAAVAAGCRQIQDle-----------iPSVEVDpc------gdA 116 cow
1LCP_A       59 e-----dfpSVVVVGLGKktagideqenwheGKENIRAAVAAGCRQIQDle-----------iPSVEVDpc------gdA 116 cow
NP_326206    45 --------eNTLYYFVKDks---------kyDIKFFKKAASKIVENQNRnl-----------nIDISSFshnsi-alesI 95  Mycoplasma pulmo...
CAD84480     68 ------hggLYAWVMVDPg-----------rSLFEQQTSLRKAMQLLLNehp---------aeIHLAVYgdd-----aqR 116 Nitrosomonas eur...
AAR38210     64 d-----ngsLVTWLMVSEk-----------kSTFQIQTLLRKAFDKIISen-----------pKTITIVneskn-ndewL 115 uncultured bacte...
YP_015871    43 ---------NKILIFIKN-------------IDELKQEKLEKIVSKILSsigs------rnvfIDLEFWgks----eekI 90  Mycoplasma mobil...
ZP_00565941  60 vsatlpsggIASWVVLDDk-----------qNTFQRHTLLRKAVSALLDdqp---------qsVTIAVFg--------eE 111 Methylobacillus ...
ZP_00640847  74 l-----aggRLIISPVTDs-----------dNEYSDVRIFAKAAREGIAiakaagatkpllvvPSVTDNrfa-----naE 132 Shewanella frigi...
YP_286161    65 ns---aeggLRVWLRLDPa-----------kSTFDNLSLLREGMAILLDeep--------atvNVLICAp---------T 113 Dechloromonas ar...
ZP_00837845  64 g----laggRLIVAPVADgds-------diqDVRGYSDAARQGIKVAFDaga---------rrPLLIVTpstqaryaqsM 123 Shewanella sp. PV-4
Feature 1                                                                                       
1LAM        117 QAAAEGAVLGLYEYDdlkqk----rkvvVSAKlhg---------------seDQEAWQRGVLFASGQNLARRLMETPANE 177 cow
1LCP_A      117 QAAAEGAVLGLYEYDdlkqk----rkvvVSAKlhg---------------seDQEAWQRGVLFASGQNLARRLMETPANE 177 cow
NP_326206    96 VKIFVEKYEFKHANLfkekdk-ddkqysINLYvsdkq-----------kilvLETLLEKTLILTNALNTVRKLQIYPPNI 163 Mycoplasma pulmo...
CAD84480    117 RHLAELAVYVAWVNGailpvrkqrkpddKPLEricl------------hgivDSTGFAMQCAQAEGNFLARGLTILPPNE 184 Nitrosomonas eur...
AAR38210    116 RQAFYVASVNSQDLPnlks-----gvkkKNLKsint------------ygglVKTNFKDIEALVSGNTLTRELTITPPNQ 178 uncultured bacte...
YP_015871    91 LEIFIEYFEEKFVYDfsyktk-ekdkiiPNLTlvt---------------siSEEIYKKMILISKSKLFVKKYQEMPPNI 154 Mycoplasma mobil...
ZP_00565941 112 ASRARAANEAFYVVSvn----------aVELPvrksrhkayplkqivihgwkQADDYAETRALVAGSALARSLTLTPPNE 181 Methylobacillus ...
ZP_00640847 133 QVAALACGQELWQPLeare-----skslQPSLtaig-------------llnTATHSLLLNAAESGRVLARDLCGTEPER 194 Shewanella frigi...
YP_286161   114 ATSGWMADLATYVGLan----------gTPLPnrkqedapr--ameslniwgSDHASEHARSLADGNLLARSLTALPPNE 181 Dechloromonas ar...
ZP_00837845 124 GVAALGCAQALWQSLerre-----vlgeMDNIsqig-------------llvDDKAGELLCAIEAGKHLARDLCGTEPER 185 Shewanella sp. PV-4
Feature 1                                                                                   #   
1LAM        178 MTPTKFAEIVEENLKsasikTDVFIRpkSWIEEQEMGSFLSVAKGSEe----pPVFLEIHYKGspnasepPLVFVGKGIT 253 cow
1LCP_A      178 MTPTKFAEIVEENLKsasikTDVFIRpkSWIEEQEMGSFLSVAKGSEe----pPVFLEIHYKGspnasepPLVFVGKGIT 253 cow
NP_326206   164 CNSEWLADFVYEDLKki-dsLKINIYnkSQIEDLGMNLFLSVNKGSAy----ePRLVSIEYNGdps-seeKTVLVGKGIT 237 Mycoplasma pulmo...
CAD84480    185 LTPRTYREQIRKLAAse--nWQYEEYelSRLRELGAGAFSAVAQGSLtd---dAAIVHLRRTAghgetgkTIALVGKGIC 259 Nitrosomonas eur...
AAR38210    179 LTPTIYRQKIKKLSKen--gWKLEEFamPKLKKLGAGAFYAVAQGSEpq---dAAIVHLTYSPkga--keTISLVGKGIC 251 uncultured bacte...
YP_015871   155 ATSEYLASVVEKEFEns-plINVKILnkQDIKNLKMGLLLAVNAGSSh----ePRVVELNYNGnle-sknRTVIVGKGIT 228 Mycoplasma mobil...
ZP_00565941 182 LTPAAYREKIKELAGqy--gWRRDEYdlKKLRKIGAGSFVAVAQGSSee---dAAIVHLSYQPkda--rqHVAVVGKGIC 254 Methylobacillus ...
ZP_00640847 195 MSAIGFAQYCVDAFAds--gLAVSVVdqVEQLKAQYPLLSAVGRSSFdvarhhPRVVKLEYVGegp-itkTLFFAGKGVV 271 Shewanella frigi...
YP_286161   182 LTPSAYRARIAELANqh--gWEMEVFdlPRLREMGAGAFASVAQGSEan---dAAIVRLSLIPpna--kaRVALVGKGIC 254 Dechloromonas ar...
ZP_00837845 186 MSAPAFAQYCVDALEgl--dLTMSVVedRDVLERDYPLLSAVGRASFavsrhqPRVVKLEYKAegp-ierSYFFAGKGVV 262 Shewanella sp. PV-4
Feature 1        #      #          #                                                           #
1LAM        254 FDSGGISIKAaANMDLMRADMGGAATICSAIVSAAKLdl-pINIVGLAPLCENMPSGKANKPGDVVRARNGKTIQVDNTD 332 cow
1LCP_A      254 FDSGGISIKAaANMDLMRADMGGAATICSAIVSAAKLdl-pINIVGLAPLCENMPSGKANKPGDVVRARNGKTIQVDNTD 332 cow
NP_326206   238 FDSGGYNIKT-SHMGGMKFDMSGAAIAAYALKAMAQLra-kSNFAALMLITDNRLNSVANIPDSVWKSMNGKTVEVTDTD 315 Mycoplasma pulmo...
CAD84480    260 FDTGGHNLKPaRYMYGMHEDMNGSAVALGVLLAATRAdl-pINIDCWLAIAQNHISPAAFKQNDVITALNGMTIEIVHTD 338 Nitrosomonas eur...
AAR38210    252 FDTGGHNLKPaKFMSGMHEDMNGSAVVLGILKAISDAkv-kIKLDCWLAIAQNHIGPKAYKQNDVVKALNGMTIEIVHTD 330 uncultured bacte...
YP_015871   229 FDTGGYNLKPsKYLSGMKFDMSGAMIASGVMKVVENLsp-kINLSVLMVLTDNKISSRGTTPEEIHYAMNGKSIEITDTD 307 Mycoplasma mobil...
ZP_00565941 255 FDTGGHNLKPaKYMHGMHEDMNGSAVALGILLAVTEAql-pVRIDCWLALAQNHIGPRAYKQSEVVTALNGTTIEIVHTD 333 Methylobacillus ...
ZP_00640847 272 YDTGGADIKVaGSMAGMSRDKGGAAAVAGLMKALSILkpkgIKVIAELGLVRNSIGSDAFVTDEIITSHAGVRVRIGNTD 351 Shewanella frigi...
YP_286161   255 FDTGGHNLKSaDSMIGMHEDMAGSAAVLGILLAASQQtq-pLRIDAWLALASNDISPRASRQGDIVTALNGTTIEIVHTD 333 Dechloromonas ar...
ZP_00837845 263 YDTGGADLKVgGAMAGMSRDKGGAAAVAGLMKTLALLkpkgVRVVAELGLVRNSIGSEAFVTDEIITSHAGVRVRIGNTD 342 Shewanella sp. PV-4
Feature 1        # #                           #                                                
1LAM        333 AEGRLILADALCYAHt----fNPKVIINAATLTGAMDIALGsgATGVFTNSs----WLWNKLFEASIETGDRVWRMPLfe 404 cow
1LCP_A      333 AEGRLILADALCYAHt----fNPKVIINAATLTGAMDIALGsgATGVFTNSs----WLWNKLFEASIETGDRVWRMPLfe 404 cow
NP_326206   316 AEGRLVLADGITFAIrk---lNATRVITIATLTGTILYTLGtsYTGGWATNn----KTWKELLSAAKKHDEPIWRLPLne 388 Mycoplasma pulmo...
CAD84480    339 AEGRLVLADTLTLAAr----eKPDLIIDYATLTGSLITALGsrYSGVFTNRd----YLAQNAVAVGRSSGERVCVLPVds 410 Nitrosomonas eur...
AAR38210    331 AEGRMVLADTLTMASr----kKPKAIIDFATLTGCMHVAMGdrYSGVLSNHs----ALGCMAVGAGSDIGERVTAFPSde 402 uncultured bacte...
YP_015871   308 AEGRLILADGISYASki---lKASEIITIATLTGSVIVALGdkISGVWSTNd----ESWKKLKKAAKISNEKIWRLPLde 380 Mycoplasma mobil...
ZP_00565941 334 AEGRMVLADTLTLASr----vKPDFMIDFATLTGSMQVALGdrYSGVIGNDn----TWLDKAVQAGHAVGERVCRLPFde 405 Methylobacillus ...
ZP_00640847 352 AEGRLVLADLLSHLRlqaqtaVNPQLFSVATLTGHVVRAYGg-YPALVENSaakkqGVAATLAEVGQLWGEPFEVSRLrp 430 Shewanella frigi...
YP_286161   334 AEGRMVLADTLALAGr----sKPDLIIDFGTLTYTMINALGcrYSGIFASSd----ELGALAVRAGSASGERVCTFPMda 405 Dechloromonas ar...
ZP_00837845 343 AEGRLVLADLLSHLRlkaqdaVAPQLFSVATLTGHVVRAYGg-YTAVVENAvarqqQVGDSLQTISEAWGEPIEVSRLrr 421 Shewanella sp. PV-4
Feature 1                                                                                       
1LAM        405 -hYTRQVid-cqLADVNNIGky----rsAGACTAAAFLKEFvth----------pkWAHLDIAGVMTNkdevpylrKGMA 468 cow
1LCP_A      405 -hYTRQVid-cqLADVNNIGky----rsAGACTAAAFLKEFvth----------pkWAHLDIAGVMTNkdevpylrKGMA 468 cow
NP_326206   389 -dYIESYkk-skVADLVNWSsa----skPDSNSAAMFLKEFtee----------kdFVHLDIAGTAEIe-------GEPQ 445 Mycoplasma pulmo...
CAD84480    411 -dYEADLds--kIADIKQCTie----seADHILAACFLRHFvnd----------ipWLHMDLSACRHQdglg-avgTEVT 472 Nitrosomonas eur...
AAR38210    403 -dYEEDLks--qIADIKQCTle----ggPDHIHATRFLGRFven---------kvpWLHVDLSSSNRKgglg-avaSEVN 465 uncultured bacte...
YP_015871   381 -dFLEGLks-srIADLINYDan----nySDSNSAAIFLKQFtnk----------sdLIHLDIAGTVSKk-------SNPN 437 Mycoplasma mobil...
ZP_00565941 406 -dYEEELes--sIADIKQCTle----geADHVLAARFLARFieh---------gvpWVHVDLSSYRRKgglg-avgSDVN 468 Methylobacillus ...
ZP_00640847 431 -eDFDKIadkngAADVVSSNngpssitaRGHQYPAAFLIKAsglte------telaFTHIDIAGSAVEgdp---lyGKPT 500 Shewanella frigi...
YP_286161   406 -dYEDALds--kVADICQCSldd---daPDHIHAARFLKRFvge----------lpWLHMDLSAASCEdglg-aiaTDQT 468 Dechloromonas ar...
ZP_00837845 422 ddYQKIVdp-sgAADVLSSNnapssvtaRGHQYPAAFLLKAsgleahgltsdkplaYTHVDIAGSATEgdp---lyGTPT 497 Shewanella sp. PV-4
Feature 1                  
1LAM        469 GRPTRTLIEFL 479 cow
1LCP_A      469 GRPTRTLIEFL 479 cow
NP_326206   446 GVLVKTLAQLA 456 Mycoplasma pulmonis UAB CTIP
CAD84480    473 GFGVTWSMHFL 483 Nitrosomonas europaea ATCC 19718
AAR38210    466 GFGVSFGVNMI 476 uncultured bacterium 580
YP_015871   438 AVLLKTLVNYL 448 Mycoplasma mobile 163K
ZP_00565941 469 GFGVSLGLQLV 479 Methylobacillus flagellatus KT
ZP_00640847 501 ASPLVALLQFS 511 Shewanella frigidimarina NCIMB 400
YP_286161   469 GFGVAWGISFL 479 Dechloromonas aromatica RCB
ZP_00837845 498 AAPLVSLCQLI 508 Shewanella sp. PV-4

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