1VZ8,1VZ8,1VZ6,1VZ7


Conserved Protein Domain Family
OAT

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cd02152: OAT 
Click on image for an interactive view with Cn3D
Ornithine acetyltransferase (OAT) family; also referred to as ArgJ. OAT catalyzes the first and fifth steps in arginine biosynthesis, coupling acetylation of glutamate with deacetylation of N-acetylornithine, which allows recycling of the acetyl group in the arginine biosynthetic pathway. Members of this family may experience feedback inhibition by L-arginine. The active enzyme is a heterotetramer of two alpha and two beta chains, where the alpha and beta chains are the result of autocatalytic cleavage. OATs found in the clavulanic acid biosynthesis gene cluster catalyze the fifth step only, and may utilize acetyl acceptors other than glutamate.
Statistics
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PSSM-Id: 239065
Aligned: 131 rows
Threshold Bit Score: 394.14
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site pocket [active site]
Evidence:
  • Structure:1VZ8_AB: Streptomyces clavuligerus OAT's active site pocket is formed by residues from two subunits; a sulfate molecule is in the pocket.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1VZ8_A      9 PRGFVVHTAPVGlad-----dgRDDFTVLASta-pATVSAVFTRSRFAGPSVVLCREAVAdGQARGVVVLARNANVATgL 82  Streptomyces clavu...
1VZ8_D      9 PRGFVVHTAPVGlad-----dgRDDFTVLASta-pATVSAVFTRSRFAGPSVVLCREAVAdGQARGVVVLARNANVATgL 82  Streptomyces clavu...
1VZ6_A      9 PRGFVVHTAPVGlad-----dgRDDFTVLASta-pATVSAVFTRSRFAGPSVVLCREAVAdGQARGVVVLARNANVATgL 82  Streptomyces clavu...
1VZ7_D      9 PRGFVVHTAPVGlad-----dgRDDFTVLASta-pATVSAVFTRSRFAGPSVVLCREAVAdGQARGVVVLARNANVATgL 82  Streptomyces clavu...
AAR05433    6 PRGFRVHHGHAGir------gsHADLAVIASdv-pAAVGAVFTRSRFAAPSVLLSRDAVAdGIARGVVVLSGNANAGTgP 78  Streptomyces clavu...
YP_464367  25 PEGFRIGTCAFEfvpvevpkpgRMRLTLVALdrptPAFAAKFTRNAFPGAPVLVGRRRLDgAALGAVVINNKVANVCA-P 103 Anaeromyxobacter d...
Q97ET6      7 PKGFLCCGKHVGik------krKLDLGVVYSek-lCSAAAVFTKNKFCGIPIIVGKENIKdNKLQSIVVTSGVANVATgE 79  Clostridium acetob...
P96426     23 PQGFHCWTTHLGlad-----egKDDFSMVISdr-pCTSSVVFTKSLFAGPCVTLSKTSIEqTSPRGVLVLAKNANVATgA 96  Rhodococcus fascians
Q8EYV8      4 PKGFLSFGINIGik------ddTKDFGVIYSei-pCKATAVFTKNNFPGAPVIVGKEHVRsGVLQAIVINSKNSNVATgE 76  Leptospira interro...
Q8TX15      4 PEGFLLGGIKRE----------GIGVGLIFSer-rCAVAGTFTENTLRAAPVEHSEEVCDrGVARGVIVNSGHANAMTgE 72  Methanopyrus kandleri
Feature 1                                 ##                                     ##           
1VZ8_A     83 EGEENAREVREAVARALglpegEXLIASTGVIGrQYPXESIREHLKTLewpa--gegGFDRAARAIXTTDTR-PKEVRVS 159 Streptomyces clavu...
1VZ8_D     83 EGEENAREVREAVARALglpegEXLIASTGVIGrQYPXESIREHLKTLewpa--gegGFDRAARAIXTTDTR-PKEVRVS 159 Streptomyces clavu...
1VZ6_A     83 EGEENAREVREAVARALglpegEMLIASTGVIGrQYPMESIREHLKTLewpa--gegGFDRAARAIMTTDTR-PKEVRVS 159 Streptomyces clavu...
1VZ7_D     83 EGEENAREVREAVARALglpegEMLIASTGVIGrQYPMESIREHLKTLewpa--gegGFDRAARAIMTTDTR-PKEVRVS 159 Streptomyces clavu...
AAR05433   79 RGYEDAAEVRHLVAGIVdcderDVLIASTGPVGeRYPMSRVRAHLRAVrgpl--pgaDFDGAAAAVLGTAGArPTIRRAR 156 Streptomyces clavu...
YP_464367 104 DGVEAAERVCAEAARLLglapeEVLPSSTGVIGwRLPVERMVEALPAAaaal--qggSALAGAEAIMTTDLY-PKLRRAE 180 Anaeromyxobacter d...
Q97ET6     80 EGLKNTYRILNKLSCELnikseNILPSSTGIIGkQLPIDCIITGINGIkssl--skyNWEEFNRAIMTTDKE-LKIKSCK 156 Clostridium acetob...
P96426     97 EGLRNASEIRASVARTVgiepdALIMASTGVIGvQYPMDTIRAGLDGLgqg---tplDAHAVARAIMTTDTR-AKVSERA 172 Rhodococcus fascians
Q8EYV8     77 KGIQNSREICKIIGESLgiketLVLPSSTGVIGvPLKMEIILPACKKAksll--kpgNLEEVAEAIMTTDTR-KKISSRN 153 Leptospira interro...
Q8TX15     73 EGYQDVLRTAEAIAELMgapedEIVVCSTGVIGeRPPVDKIVRYAREVwedigpterHVREFSRAIMTTDTE-EKIALYE 151 Methanopyrus kandleri
Feature 1                   #  #         #                                                    
1VZ8_A    160 Vg----gATLVGIAKGVGXLEPDX--ATLLTFFATDARLDPAEQDRLFRRVXDRTFNa-VSIDTDTSTSDTAVLFANGLA 232 Streptomyces clavu...
1VZ8_D    160 Vg----gATLVGIAKGVGXLEPDX--ATLLTFFATDARLDPAEQDRLFRRVXDRTFNa-VSIDTDTSTSDTAVLFANGLA 232 Streptomyces clavu...
1VZ6_A    160 Vg----gATLVGIAKGVGMLEPDM--ATLLTFFATDARLDPAEQDRLFRRVMDRTFNa-VSIDTDTSTSDTAVLFANGLA 232 Streptomyces clavu...
1VZ7_D    160 Vg----gATLVGIAKGVGMLEPDM--ATLLTFFATDARLDPAEQDRLFRRVMDRTFNa-VSIDTDTSTSDTAVLFANGLA 232 Streptomyces clavu...
AAR05433  157 Cg----dATLIGVAKGPGTGPAEQddRSTLAFFCTDAQVSPVVLDDIFRRVADRAFHg-LGFGADASTGDTAAVLANGLA 231 Streptomyces clavu...
YP_464367 181 Vg----gGSIVGFAKGAGMVEPNL--ATMLVYLLTDLDVPRDALRAALDRAVEGSFNr-ISVDSDTSTSDTVVLLSSRKR 253 Anaeromyxobacter d...
Q97ET6    157 Ig----dATVLGIAKGSGMIEPNM--ATMLAYFFTDASIKAVELKGILKRAVDKSFNm-ISIDHDTSTSDTAAILANGYV 229 Clostridium acetob...
P96426    173 Vg----tSTIVGIAKGVGMIEPDM--ATMLSFVFTDADVPQDTLNRIFRDVVDRTYNs-VSIDTDTSTSDTAAVFANGSA 245 Rhodococcus fascians
Q8EYV8    154 IktksgqGTIYGIAKGAGMIEPNM--ATMLCYILSDVSLPEGTDLYSILKSSVDQSFncLTIDSDTSTSDTVALLCNGLS 231 Leptospira interro...
Q8TX15    152 Gd----gWSLLGIAKGAGMIHPNM--STMLAFLLTDVGAKPKELQMWLRDVVNDTFNm-ITVDGDESTNDSVVLLANGSS 224 Methanopyrus kandleri
Feature 1                                                                                     
1VZ8_A    233 ge-----vDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVA 307 Streptomyces clavu...
1VZ8_D    233 ge-----vDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVA 307 Streptomyces clavu...
1VZ6_A    233 ge-----vDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVA 307 Streptomyces clavu...
1VZ7_D    233 ge-----vDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVA 307 Streptomyces clavu...
AAR05433  232 gr-----vDLVAFEQVLGALALDLVRDVVRDSGCGGALVTVRVTGAHDTEQAGRVGRAVVDAPSLRAAVHGPAPDWAPVA 306 Streptomyces clavu...
YP_464367 254 pa-----pPAGDFAAALARVCGDLAEDVVRNGEGVHHVVRVAVRGAPSDAAAHLVGKAVVNSPLLKAAVNGNDPNVGRLL 328 Anaeromyxobacter d...
Q97ET6    230 gn-----vNLEVFEKTFTDMCIDMAKDIVRDGEGVSKLIEATVMGCSSFESAKVIAKSIINSPLVKIAIYGSDPNWGRIA 304 Clostridium acetob...
P96426    246 gp-----vPDTEFEQALEEVCTDLVKMIASDGEGATKLIVTTVSGASSERQARVVGKSIINSPLVKTMIHGEDPNWGRVL 320 Rhodococcus fascians
Q8EYV8    232 ge-----sSVQDFSKALTEICIDLTKLIATDGEGATKLIELTISGAKSEAQARKIGKSILNSPLVKTAIYGGDPNWGRLI 306 Leptospira interro...
Q8TX15    225 nlkvgsdvTITEFQRALEEVCTELAEKIVRDGEGATKLMIVCVHGASNEVEARRAARAIASSNLVKAALFGENPNWGRIG 304 Methanopyrus kandleri
Feature 1                                                                                     
1VZ8_A    308 XAIGKCSddtd-idqERVTIRFG--------EVEVYppkargdqaddaLRAAVAEHLrg--------------------- 357 Streptomyces clavu...
1VZ8_D    308 XAIGKCSddtd-idqERVTIRFG--------EVEVYppkargdqaddaLRAAVAEHLrg--------------------- 357 Streptomyces clavu...
1VZ6_A    308 MAIGKCSddtd-idqERVTIRFG--------EVEVYppkargdqaddaLRAAVAEHLrg--------------------- 357 Streptomyces clavu...
1VZ7_D    308 MAIGKCSddtd-idqERVTIRFG--------EVEVYppkargdqaddaLRAAVAEHLrg--------------------- 357 Streptomyces clavu...
AAR05433  307 AVAGGHGdegpgrspGRITIRVG--------GREVFpa--------prDRARPDAVTayp-------------------- 350 Streptomyces clavu...
YP_464367 329 CAVGKVA--------GAAGIALDparavmrvGGEVVleggam-rldpeKERRLVAHMkaaelyasapppdgvsfrppidh 399 Anaeromyxobacter d...
Q97ET6    305 MAIGKSFedd--vdpLKIEIAFN--------SNVIYdkgk----iyeeNFDCIERYLrd--------------------- 349 Clostridium acetob...
P96426    321 MAIGKCSnevd-iepDRIRVAFA--------DIDVYpsssl---dqhdTVEVVREHLat--------------------- 367 Rhodococcus fascians
Q8EYV8    307 MAVGKVFdep--ipfEGLQIYFG--------TLPVKean-------peTLKKLSEYLkn--------------------- 348 Leptospira interro...
Q8TX15    305 AAVGAARvdv---dpDELRIAFRs------sEGEIVtyegg---pvdfDEEKAKRVLsa--------------------- 351 Methanopyrus kandleri
Feature 1                                          #   ##
1VZ8_A    358 ----dEVVIGIDLa---iADGAFTVYGCDLTEGYVRLNSEYTT 393 Streptomyces clavuligerus
1VZ8_D    358 ----dEVVIGIDLa---iADGAFTVYGCDLTEGYVRLNSEYTT 393 Streptomyces clavuligerus
1VZ6_A    358 ----dEVVIGIDLa---iADGAFTVYGCDLTEGYVRLNSEYTT 393 Streptomyces clavuligerus
1VZ7_D    358 ----dEVVIGIDLa---iADGAFTVYGCDLTEGYVRLNSEYTT 393 Streptomyces clavuligerus
AAR05433  351 --hggEVTVHIDLgvpgrAPGAFTVHGCDLLAGYPRLGAGRAV 391 Streptomyces clavuligerus
YP_464367 400 ppherRVEIDIDLg---mGAGACEVLGGDLSHEYVTENADYRS 439 Anaeromyxobacter dehalogenans 2CP-C
Q97ET6    350 ---nnECKIQANLn---vGDFAATVWGSDFTEDYIRINSYYTK 386 Clostridium acetobutylicum
P96426    368 ----vTVEINVDLg---iGTDTWRVFGCDLTDEYIRINADYTT 403 Rhodococcus fascians
Q8EYV8    349 ---ntEISLNVVLn---vGTISMKFWGCDFTEKYIEENAYYTT 385 Leptospira interrogans
Q8TX15    352 ----sEVEIVVDLg---vGDASARAWGCDLTYEYVRINAEYRT 387 Methanopyrus kandleri

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