1W2Y,2YAY,2CJE,2CIC


Conserved Protein Domain Family
NTP-PPase_dUTPase
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cd11527: NTP-PPase_dUTPase 
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Nucleoside Triphosphate Pyrophosphohydrolase (EC 3.6.1.8) MazG-like domain found in dimeric 2-Deoxyuridine 5'-triphosphate nucleotidohydrolase and similar proteins
dUTPase (dUTP pyrophosphatase; EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. It acts to ensure chromosomal integrity by reducing the effective ratio of dUTP/dTTP. Members in this family are dimeric dUTPases, such as those from Leishmania major, Trypanosoma cruzi, and Campylobacter jejuni, which differ from the monomeric and trimeric forms and adopt an all-alpha topology. A central four-helix bundle, consisting of two alpha-helices from the rigid domain and two helices from the mobile domain and connecting loops, form the active site in dimeric dUTPase-like proteins, requiring the presence of metal ion cofactors to hydrolyze both dUTP and dUDP.
Links
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Statistics
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PSSM-Id: 212134
Aligned: 53 rows
Threshold Bit Score: 56.0705
Created: 29-Aug-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The dimeric dUTPases consist of five active site-forming helices. The divalent metal ions, such as Mg2+(s), are required for activity.
  • Structure:1W2Y; Campylobacter jejuni dUTPase binds dUpNHp/Mg2+ complex, contacts at 4A.
  • Structure:2YAY; Leishmania major dUTpase binds dUPNPP/Ca2+ complex, contacts at 4A.
  • Structure:2CJE; Leishmania major dUTpase binds dUTPase binds dUpNHp/Mg2+ complex, contacts at 4A.
  • Structure:2CIC; Campylobacter jejuni dUTpase binds dUpNHpp/Mg2+ complex, contacts at 4A.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1            #  ##  ##                       #      #  #                               # 
1W2Y_A        10 MLKLQQKLNDETNglnwengy-------tkegkliSWRRCIYMECAELIDSFt--WKHWKNIsslt-----nwenVRIEI 75  Campylobacter j...
NP_853142      8 LIEKQAELDTIIFkktkss----------ykknqkQRRLWLAVEIGNLAKQIe-aYKFWKNGpked------dhaILEAC 70  Mycoplasma gall...
YP_004398476   6 LIASSVYLNRQIEskkqlhw--------sndgrvkNAFVALDVELAEMANTSe-wFKVWKTHrgkhdpnktpretLLYEY 76  Lactobacillus b...
AEV95353       6 MLKTSVDLDRQIRekhnlqe--------siderieNCYLALDTELSEVANTSe-wFKVWKTHagkhdegltvdetVLNEV 76  Pediococcus cla...
CBI48237      13 LLQIQKEFDDRIPtln-----------------lgDSKIAYVVEFFEWFNTLe-tFKNWKKKpgkp------ldvQLDEL 68  Staphylococcus ...
NP_785180      7 LLQQSIRLDRDITarqqihw--------rpqerlqNAMVSLDVELAEMANTSe-wFKVWKIHrgkadpdkthretLLNEY 77  Lactobacillus p...
YP_002560318   9 FSKKQEELDSMIRekfeisee------vwkydlnvQHSIALRVELHELVNEChdlWKYWKQKavn-------pdrIIDEL 75  Macrococcus cas...
YP_004191946  10 LLEGQAKLDKYIFekkgtd----------ylktssFRRLALLVELGELANACk-pFKFWSVSdpn-------meeVLDEY 71  Mycoplasma haem...
EGO87963       6 FLDKQGQLDRYILnnkevni--------sdkelltDTLLALQVEVSELANATr-vFKYWSNKgpes------rerILDEY 70  Clostridium bot...
YP_003936565   6 LVVKQKYLDDVVIknynsnhsdklgfyldhkdfitHRLLGLVVELAEVCNEQd-lHKYWKNNkkv-------ggnTLEEL 77  Clostridium sti...

Feature 1         #  ##                                                                          
1W2Y_A        76 VDIWHFILSLLLEEyrdknnkdfkaiatevnavsvfqdfckeeeypnegdiygilndieliihkcsgfgfnlgellstyf 155 Campylobacter j...
NP_853142     71 ANVLHLSLSFLIQFkteidqdkktelnfvfdvkqqkqkpssliaskrflel--------yelclsmedwwscqkfidell 142 Mycoplasma gall...
YP_004398476  77 VDAMDFYLLISNLKnwnhvilnsqddldkikqfkkednldkqylalkrmlf---------dayfdhsgdsfshswrlflk 147 Lactobacillus b...
AEV95353      77 GDVMDFFLLLAYLNqwnhlivieeheidayqndsrkmnltktflnikkmlf---------gaysynrstdyqhawhlfik 147 Pediococcus cla...
CBI48237      69 ADMLAFGLSIANQVgvsseeikeaiessfkdtefhkmfnfkdkefaqgavv---------stpqiifkefypdqlaivia 139 Staphylococcus ...
NP_785180     78 VDAMDFFFLVSAIQqwthlipvtpeqlttiaakpatdlnkqylaikhlly----------dayfnhrqesyqhawhvflk 147 Lactobacillus p...
YP_002560318  76 VDVIHFLHLILNKMnydadyhvreinknipndeqtkyvtdtlggvfrv---------------nyliksdelhetyahll 140 Macrococcus cas...
YP_004191946  72 ADIMHFVLAISLERnydlsgikfldspevnkevltdvflnlilgfs------------------lcksqddfkncvskfl 133 Mycoplasma haem...
EGO87963      71 SDIVHFLLSIANQLgftvr------------------------------------------------------------- 89  Clostridium bot...
YP_003936565  78 ADVLHFLLSITHTLgysga------------------------------------------------------------- 96  Clostridium sti...

Feature 1                           #   #  #    #
1W2Y_A       156 tlaikcglnleilyktYIGKNVLNIFRQNNGY 187 Campylobacter jejuni
NP_853142    143 tlislmrlnfndlfkeYIRQSANILKRIDEDI 174 Mycoplasma gallisepticum str. R(low)
YP_004398476 148 fglvdfgyteqeieqaFKDKNKINEDRQDQDY 179 Lactobacillus buchneri NRRL B-30929
AEV95353     148 fclnglkftpkqmeeaFFNKNTINYKRQENNY 179 Pediococcus claussenii ATCC BAA-344
CBI48237     140 idiaynlytidqlidaYKKKMKRNHERQDGTA 171 Staphylococcus aureus subsp. aureus TW20
NP_785180    148 iglvdfgftqdeiqtaFTAKNHVNEQRQANNY 179 Lactobacillus plantarum WCFS1
YP_002560318 141 vladryaftlddieqaYDRKNAENHKRQESGY 172 Macrococcus caseolyticus JCSC5402
YP_004191946 134 elgsmlgfsfekiekaYWDKWQVNFDRQNSNY 165 Mycoplasma haemofelis str. Langford 1
EGO87963      90 -----------dieeaYLKKHKENYRRQQEGY 110 Clostridium botulinum C str. Stockholm
YP_003936565  97 -----------dletaYLEKFQENLRRQENGY 117 Clostridium sticklandii DSM 519

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