2WZV,3PXV,5J62,3GFA,3GR3


Conserved Protein Domain Family
PnbA_NfnB-like
?
cd02136: PnbA_NfnB-like 
nitroreductase similar to Mycobacterium smegmatis NfnB
Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.
Links
?
Statistics
?
PSSM-Id: 380313
Aligned: 56 rows
Threshold Bit Score: 116.533
Created: 13-Jan-2005
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
FMN bindinghomodimer
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:2WZV: The Mycobacterium smegmatis nitroreductase NfnB homodimer binds two molecules of FMN, contacts at 4A
  • Comment:Based on similarity to sequences with known structure and function.
  • Citation:PMID 9654450
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1            ### #                      #####                                            
2WZV_A        22 LIKGRRAVRAFRPDEVPEETXRAVFELAGHAPSNSNTQPWHVEVVSGaa----rDRLAEAlvtahaeervtvdfpyregl 97  Mycolicibacteri...
AAG14888       8 VIATRHSTRKFKPEMVEESLLEKVIEAGRRAPSGKSKNTNHFLVIRDaa---vlNKLVELvqsefakmditpenqd---- 80  Selenomonas rum...
NP_771292     12 AIYSRHAVREFTSQPVDEDVLRKLIDAAIQAPSAVNHQPWLFTVVRDk------TLLARIsreakahmlktsaaal---- 81  Bradyrhizobium ...
AAM05179      37 NIYQRRSVRNYSDKEVSDEIIKEIIRAGTYAPSAVNKQPWRFVVVKNrqlidkyDDLARKaflsvygdtd---------- 106 Methanosarcina ...
NP_953571      6 GILSRRSVRQFTDEPVSPAEVEDILRAGAWAPSGLNNQPWRFAVVRDr------EKLRSLagltr--------------- 64  Geobacter sulfu...
AAM07140      12 TILNRRSVREFTDRPISKEEINTILNAGRWAPSGLNNQPWRFAVVRNp------ETIQKLsecth--------------- 70  Methanosarcina ...
WP_093371814  12 LVFKRRSCRIFTDTPVTPEELDSILRAGCCAPTSKALYPCHFLVVDSr------SEMQKIceyhn--------------- 70  Ruminococcaceae...
AAL95419       7 VIRERRSIRKFRSDMLPKEIIDKVIESGLYAASGKGQQSPIIISITNke---lrDKLSKMnck----------------- 66  Fusobacterium n...
CDB41895       8 MIKTRRSTRSFKPDSIPEDLLDAVLEAGTYAPTGMNRQSPVIIAVSDkk---yrEKLSKLna------------------ 66  Ruminococcus sp...
WP_002702184   6 ELKSRRSVRAFSEEKIAKEDIDKIIEAGIFAASGMNKQPTKIVCVTDks---vrDTLSRLnae----------------- 65  Treponema sacch...

Feature 1                                                #                           #  #        
2WZV_A        98 fqgvlqerradfgsrlyaalgiardqtdllqgynteslrFYGAPHVAXLFAPNn-------TEARIAGDXGIYAQTLXLA 170 Mycolicibacteri...
AAG14888      81 -----------------------nvggaiklskkgnyvfKYNAPVLIVVANKK--------DYGNNMADACAAVENMLLA 129 Selenomonas rum...
NP_771292     82 ------------------------ashhfqsilenpdfdIFYHAPALVVISAP--------EGPWVVEDCALAAENLMLA 129 Bradyrhizobium ...
AAM05179     107 ------------------------------npdligyvqYLSKPTTRILYGAPvfilvfaaPDVIDDRDCALAAENMMLA 156 Methanosarcina ...
NP_953571     65 -----------------------------------yrhiVEGAPVSIAVFCDRda----myHETKDHQAIGACLQNMLLA 105 Geobacter sulfu...
AAM07140      71 -----------------------------------ysgiVAGAALLIAAFLDTeh----syNRTKDIQAIGASIQNMLLA 111 Methanosarcina ...
WP_093371814  71 -----------------------------------mskaLVTAPLCVITCADTa------rSWASWRDDAAAATMNMSLC 109 Ruminococcaceae...
AAL95419      67 -------------------------------------igGWKEDFDPFYNAPVvlvvlapkDWPTYIYDGSLVIGNMMLA 109 Fusobacterium n...
CDB41895      67 -------------------------------------aiMGVDKDPYYGAPTVvlvl-adpSANTFVEDGSCVLENLMLA 108 Ruminococcus sp...
WP_002702184  66 -------------------------------------amGKSGIDPFYGAPVVlavl-sdsESHTYIEDGALVMGNMMNE 107 Treponema sacch...

Feature 1                #####                             #                  # #       
2WZV_A       171 XTAHGIASCPQALLS------FYADTVRAELGVe---nRKLLXGISFGYADdtaa-vngVRIPRAGLSETT 231 Mycolicibacterium smegma...
AAG14888     130 ANALDLGACWINQLRwl---rDNPVLLAYLQSLgmkedEWVCASVSLGYPDmpdglpnrNITTIKGNEVTY 197 Selenomonas ruminantium ...
NP_771292    130 ACAAGLGTCWIGFAQgw---lGTSEGKSSLRLSg---tTVPVAPIIVGHPKta----aaPVPRKAPKIDWI 190 Bradyrhizobium diazoeffi...
AAM05179     157 ARSLGIGSCWIGLAAgl---gSDTEFLKEVGVPe---gYKLIAPLIFGYPAk------dNQKAPARNADVI 215 Methanosarcina acetivora...
NP_953571    106 AHALGLGAVWLGEILk-----SAGQVRDLLGLPp---nLELMAVIAVGRPSgv-----pHHSDRKPLSELI 163 Geobacter sulfurreducens...
AAM07140     112 CCGLSLGAVWLGEILn-----QHEKVNSILGCSs---kLELMAVLAIGEPApgkrtstrKALPELVFEEKY 174 Methanosarcina acetivora...
WP_093371814 110 AEALGLNSCWLGVYPrv---eRVNRMKEQFSLPd---pIIPYSILALGHADspkpplgrFDPSCVHYNRWQ 174 Ruminococcaceae bacteriu...
AAL95419     110 AHSLNIGSCWIHRAKqefeseEGKEILKSLGINg---eYEGIGHCVLGYIDgn----ylNTPARKENRVFY 173 Fusobacterium nucleatum ...
CDB41895     109 AHACDLGSVWVHREReif-dsEEGKKLLREWNLp----ETLRGVGSIALGYsaa---epTARPPRKDGYIV 171 Ruminococcus sp. CAG:177
WP_002702184 108 AHSIGIGSCWIHRGRevf-dsDEGKALKEKWGIp----EGYRGIAFLILGHaak---egPAPARKENRVLY 170 Treponema saccharophilum

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap