4P0P,2ZIV,2ZIW,2ZIU


Conserved Protein Domain Family
XPF_nuclease_EME1
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cd20081: XPF_nuclease_EME1 
XPF-like nuclease domain of crossover junction endonuclease EME1
EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. The nuclease domain of EME1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA.
Links
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Statistics
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PSSM-Id: 410857
Aligned: 14 rows
Threshold Bit Score: 277.787
Created: 15-Nov-2018
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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nucleic acidheterodimer
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleic acid substrate binding site [nucleic acid binding site]
Evidence:
  • Comment:DNA-binding induced conformational changes in the linkers connecting the nuclease and nuclease-like domain of Mus81 and Eme1 facilitating the DNA substrate bending and ultimately place the incision strand at an active site of Mus81.
  • Structure:4P0P; Homo sapiens Mus81-Eme1 complex binds flap DNA substrates; contacts at 4A.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
4P0P_B        67 RPEECLKHIIVVLDPVLLQmeGGGQLLGALQTmECRCVIEAQaVPCSVTWRRRagpse-----dredWVEEPTVLVLLRA 141 human
2ZIU_B        15 NSEECLKHIIVVLDPVLLQmeGGGQLLGALQTmECRCVIEAQaVPCSVTWRRRagpse-----dredWVEEPTVLVLLRA 89  human
XP_014346050 276 RPEECMKHMVVAIDPALLEieGGGQLLSALQSiECSYMIEGQrIPRCMTWKRKplrtq----vestvWKDEPNILVQVPL 351 coelacanth
XP_008102713 254 RPEQCLKHIQVVLDPGLLQveGGGQVLTTLQAmECTCVIESQsVPCSLTWRRKtrlaq----aeeesLTEESNLLVLVPL 329 green anole
XP_007656593 168 RPEECLKHIAVLLDPALLQleGGGRILSALQAtECACEIEAQtIPCSMAWKRRaalgq----vaeggWTEEPGVLVVLPA 243 platypus
XP_007887108 103 KPNHCLSHMLLSIDPGLLEedVGGETLSALQAtQSSCVVEEQaVGRSVTWRRRrvqpgaggehtsedWVEEPHVLVHVPL 182 elephant shark
XP_020385312 288 RPGECLKHMLVSIDPGLLQvdGGGQLLSTLQTmEVKCVIENQtIPHSMTWKRRqaciagqlmnektdWVEELHVLIYIPL 367 whale shark
AWP17211     230 RPEECIKHMVVAVDPALLQleGGGTLLASVQAlGCSCAIEKQpLPRSVSWMRRvpcaq----pddgvCEPEACVVMQMTV 305 turbot
Q96AY2       244 RPEECLKHIIVVLDPVLLQmeGGGQLLGALQTmECRCVIEAQaVPCSVTWRRRagpse-----dredWVEEPTVLVLLRA 318 human
Q5NVA9       245 RPEECLKHIIVVLDPVLLQmeGGGQLLGALQSmECRCVIEAQaVPCSVTWRRRagpsevl-wqdgedWVEEPTVLVLLRA 323 Sumatran orangutan

Feature 1                                                                                        
4P0P_B       142 EAFVSMIDNGKQgsldstmkgKETLQGFVTDITAKTaGKALSLVIVDQEKCFSaqnpprrgkqgankqt-------kkqq 214 human
2ZIU_B        90 EAFVSMIDNGKQgsldstmkgKETLQGFVTDITAKTaGKALSLVIVDQEKCFSaqnpprrgkqgankqt-------kkqq 162 human
XP_014346050 352 EEFVAMVHAYKQetvgsytegQVSLKGFVQDIIHKSeGRTPSLAVVEMEKYFRsqkshsqkklrqavlneqr-gignkrr 430 coelacanth
XP_008102713 330 EEFLAMIQNCKQvsm---erpTQTLQTFVANILQKNsGKTLALAVVELEKYFSshkpkglkkvkqavqsgsetqelgkqr 406 green anole
XP_007656593 244 EEFVSLVHGPEQggh---regKGSLRSFVADATARIpGKTLSLAVVGVEKLCRrpgprsrapaqarr-----------rp 309 platypus
XP_007887108 183 EGFLSMVHTYSQevrgadtegQVTLQSFSQSILDRSaGRIPALVVMGLEQYFRtqrkrlvteqggd-------------r 249 elephant shark
XP_020385312 368 EEFVSMVYNSKQevqgastegHTTLQSFAHDILQKSaSRIPCFVVVELERYFRpqkkqrsqkkhqhtvpne---eqdkqr 444 whale shark
AWP17211     306 DDFSSLIHSYIQeerhggsdsGPTLTSWVQQRQGCNpGKNLSLVVIDMEKYFRskksqsqkrlrvavaneeqgggkarkr 385 turbot
Q96AY2       319 EAFVSMIDNGKQgsldstmkgKETLQGFVTDITAKTaGKALSLVIVDQEKCFSaqnpprrgkqgankqt-------kkqq 391 human
Q5NVA9       324 EAFVSMIDNGKQgsldstmkgKETLQGFVTDITARTaGKALSLVIVDQEKCFSaqnpprrgkqgankqt-------kekq 396 Sumatran orangutan

Feature 1                                                           #       #   
4P0P_B       215 qrqpeasigsmVSRVDAEEALVDLQLHT--EAQAQIVQSWKELADFTCAFTKAVAEAPFKKLR 275 human
2ZIU_B       163 qrqpeasigsmVSRVDAEEALVDLQLHT--EAQAQIVQSWKELADFTCAFTKAVAEAPFKKLR 223 human
XP_014346050 431 kqkgtekhlpeISRVDVEEALVDLQLHA--GIQVRFLETWKEFADFISMFTKAVAETPFKRDR 491 coelacanth
XP_008102713 407 nwrgkansaleLSRVDVEEALVDLQLRT--GVQVQMLESWKEFADFASMFTKAVAEAPFKRER 467 green anole
XP_007656593 310 rvaredpsgpsLSRVAVEEALVELQLEAhaPVQVRLLESWQELADYACMFTKAVAEAPYKHLR 372 platypus
XP_007887108 250 pmrrkqksrvrVSRVDVEVALVHLQLTT--GVQVQFLETWKEFADHINRFTKAVAETPFKRGR 310 elephant shark
XP_020385312 445 kakgcdhhlplISRADVELALVHLQLSV--GTQFQLLETWKEFADYISMFTKAVAEAPFKKAR 505 whale shark
AWP17211     386 rknggaealpeVSRVEVEEALVHLQLHT--GVSVRFLPTWKDFSDYITMTTKAVAEAPFKYAR 446 turbot
Q96AY2       392 qrqpeasigsmVSRVDAEEALVDLQLHT--EAQAQIVQSWKELADFTCAFTKAVAEAPFKKLR 452 human
Q5NVA9       397 qrqpeasigsmVSRVDAEEALVDLQLHT--EAQAQIVQSWKELADFTCAFTKAVAEVPFKKLR 457 Sumatran orangutan

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