Conserved Protein Domain Family
Egl_like_exo
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cd06148: Egl_like_exo 
DEDDy 3'-5' exonuclease domain of Drosophila Egalitarian (Egl) and similar proteins
The Egalitarian (Egl) protein subfamily is composed of Drosophila Egl and similar proteins. Egl is a component of an mRNA-binding complex which is required for oocyte specification. Egl contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific YX(3)D pattern at ExoIII. The motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The conservation of this subfamily throughout eukaryotes suggests that its members may be part of ancient RNA processing complexes that are likely to participate in the regulated processing of specific mRNAs. Some members of this subfamily do not have a completely conserved YX(3)D pattern at the ExoIII motif.
Links
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Statistics
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PSSM-Id: 99851
Aligned: 16 rows
Threshold Bit Score: 192.114
Created: 14-Apr-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1:catalytic site [active site]
Evidence:
  • Comment:The catalytic site is characterized by the presence of four negatively charged residues (DEDD) that serve as ligands for the two metal ions required for activity.
  • Comment:Most DEDDy exonucleases contain a conserved tyrosine at the ExoIII motif which is important in catalysis.
  • Citation:PMID 9396823
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                 # #                                                    
NP_726360    541 FVTDIILKYaak-----neSIVVSLDCEGINLGLKGEITLIEIGtt-----------rgEAFLFDVQSCpa----mVTDG 600 fruit fly
XP_001349559  55 KECVDEIKNgnynllkdfnMKMIGLDIEGYKIGKYGIVSIIQICy-------------eDIYIFDIYKCdn---vyLFIN 118 Plasmodium falc...
XP_640019    175 NYAIHEIKK----------EKLIGLDIEAIEMGKKGDISLVQIStp-----------ngRIYLFDIIKMgan--vtPFKY 231 Dictyostelium d...
XP_001349283  86 KECEEAAEEin-------rSEIIAIDFEGTNLGRYGKVCLMQVYvekknmneqsdnilqKYYIFDLLKT-------SVIK 151 Plasmodium falc...
XP_783820    167 DQAIADMEQ----------QSAIGLVLKGSRLGRKGKLSLVLVLcd------------eQVYMFDVLAVp-----sLFTR 219 purple urchin
BAF85213      89 GAAILHIKK----------QNVLSVAAEGANVCRHGKLCWLQVAtn------------cRVYLFDIFLLgs----rAFHN 142 human
NP_001084849 111 GPAIRHLQN----------QKVISIGAVGQNICRHGKLSWLQFAtr------------sRVYLFDVLVLgs----kVFKN 164 African clawed ...
CAL56246     180 KTCVEAMRE----------ADVVAVDCEGVMMSRTGPITVLQCAtr------------dKIYLIDIQALgvkafgaRGSG 237 Ostreococcus tauri
CAG10654      18 GAAVIHIKQ----------QCVIGVGAGGLKMSEHGRLCWLQIAtk------------kRVYLFDILLLgs----mAFRN 71  spotted green p...
XP_001899138 311 VNAEIDSME----------ERVIGVDFKTVTLGVQGEEFLSLVVlat----------tmQIVVFDVVHSdt----iLLES 366 agent of lympha...

Feature 1                             #                                                          
NP_726360    601 GLKTVLEH-DQVIKVIHDCRNDAANLYLQFGILLRNVFDTQAAHAILQYQEsg-kqVYKAKYISLNSLCEQYNAPCNp-- 676 fruit fly
XP_001349559 119 YIKDILEC-DDIIKVTHDCREDCSILYNQYNIHLKNILDTQVAYNLLLKNNn---nYTNTYQISYDDLLKKYLFINNn-- 192 Plasmodium falc...
XP_640019    232 GLKEVLES-VKILKVVHDCRRDSEILFHRYQVALAHVYDVQIAHALVQKKIqgnipIRRYGFNELIDLYTSRKYSEIci- 309 Dictyostelium d...
XP_001349283 152 SAQKIIEN-KKTLKLIHDCREDSSALYNQLGMKLENVYDTSRAHLLLMEKQk----KNDIYQVSFAQLINDYLGINDa-- 224 Plasmodium falc...
XP_783820    220 KFIDILQA-TNITKVIHDCRFVSDLLYHHYGIELNSVFDTQVGDILIKRRQym--gDFPRNVSGTTQCILEYLEISIhd- 295 purple urchin
BAF85213     143 GLQMILED-KRILKVIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMEtg--gYLPNCITTLQESLIKHLQVAPky- 218 human
NP_001084849 165 GLQMVLED-KGILKVIHDCRWLGDILSHQYGIILNNVFDTQVGDVYLFSMEtg--gFLPHGTRTLEECLIHHLSMLPsk- 240 African clawed ...
CAL56246     238 GMRDLLESrEAPLKLMFDCRMDSDALFHQYDVRLENVMDVQILDLATRRALg----LMIDRVAGIAKCTDKHLTEAEtav 313 Ostreococcus tauri
CAG10654      72 GISSILES-KEILKVLHDCREIAGFLMGQFGVKLNNVFDTQVADLMCFHSAtg--gFFPDTVSSLEQALSSHLQVPSs-- 146 spotted green p...
XP_001899138 367 GVKEILES-EKIIKVIHDAKRVATLLAHRYAINLRKIFDTQVAHAILQYEKfg-kaVSDMRTMSFVNLRRVYYPQSI--- 441 agent of lympha...

Feature 1                                          #   #                                         
NP_726360    677 ----IKDQLKQIYRRDQKFWAKRPLT---REMMLYAAGDVLVLIHDQLFGNLArqik------penrALFSELCTEQILM 743 fruit fly
XP_001349559 193 ----HKIYFHKMITLDNYIYLKRPIM---KELISYAIQDVIYLKPLILCILDKfiik-------qkkKEEQEKNKYVNDK 258 Plasmodium falc...
XP_640019    310 ---dIKFKVKQQFENQPEVWAKRPLP---KDFIDYASLDAACLLPIYYHIKPKlqsnfd---rrflkTHFNEQLTYFKDS 380 Dictyostelium d...
XP_001349283 225 ----SLSFIKKEMYKNEKIWETRPLS---NISIIYALKNVKYLYKLYKIFDKLlpkklvl-ekskefVNYCYLNYEYKLP 296 Plasmodium falc...
XP_783820    296 --iaLHLENTQRIEEDESSWFQRPLP---KVNIRCALLDTVYLLRLREAITEQlmae-------ftcAIDIYLTVERDRL 363 purple urchin
BAF85213     219 --lsFLEKRQKLIQENPEVWFIRPVS---PSLLKILALEATYLLPLRLALLDEmmsd-------lttLVDGYLNTYREGS 286 human
NP_001084849 241 --vsFLAHRQTLTKEYHDIWFDRPMD---PTLLKLLSLEVTYLMPLRSAMLDAmfsd-------ftlLVDGYLNAYRRGT 308 African clawed ...
CAL56246     314 aadlKVRVRKLYAVEESQLWAERPLT---EDARRYAALDVWLLIKLYDKMKFDl-------------RDDKDDWTSRSLK 377 Ostreococcus tauri
CAG10654     147 ----HLLALQTQSQLTQGAFALPPELrelERIPQERREQAINRYPVNEQGLLArfspkaqpgpqtspAAQKPIHTEASSS 222 spotted green p...
XP_001899138 442 ----MMSDVTPRKLTHLPSWGLRPIT---EDFLVTVVEEAHCLVTVLYRMISAmlpk-------hllALFEEKCLKALIP 507 agent of lympha...

Feature 1         
NP_726360    744 Q 744 fruit fly
XP_001349559 259 Q 259 Plasmodium falciparum 3D7
XP_640019    381 I 381 Dictyostelium discoideum AX4
XP_001349283 297 N 297 Plasmodium falciparum 3D7
XP_783820    364 E 364 purple urchin
BAF85213     287 A 287 human
NP_001084849 309 A 309 African clawed frog
CAL56246     378 E 378 Ostreococcus tauri
CAG10654     223 S 223 spotted green pufferfish
XP_001899138 508 N 508 agent of lymphatic filariasis

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