1VR3,1ZRR,5I8S,4QGN,4QGL


Conserved Protein Domain Family
cupin_ARD

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cd02232: cupin_ARD 
acireductone dioxygenase (ARD), cupin domain
Acireductone dioxygenase (ARD; also known as 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase) catalyzes the oxidation of 1,2-dihydroxy-3-keto-5-methylthiopentene to yield two different products depending on which active site metal is present (Fe2+ or Ni2+) as part of the methionine salvage pathway. The ARD apo-enzyme, obtained after the metal is removed, is catalytically inactive. The Fe(II)-ARD reaction yields an alpha-keto acid and formic acid, while Ni(II)-ARD instead catalyzes a shunt out of the methionine salvage pathway, yielding methylthiocarboxylic acid, formic acid, and CO. ARD belongs to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization
Statistics
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PSSM-Id: 380360
Aligned: 195 rows
Threshold Bit Score: 99.9244
Created: 4-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
metal bindingactive site
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1: metal binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:[QH] [NH] [QDEN] [HQ]Click to see conserved feature residue pattern help
Evidence:
  • Structure:4QGN: Homo sapiens acireductone dioxygenase binds iron; contacts at 4.0A
  • Structure:5I8S: Mus musculus acireductone dioxygenase binds nickel; contacts at 4.0A
  • Comment:The four-coordinate metallocenter usually includes three histidines and one glutamate.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1VR3_A        40 LEQLRTlgVLYWKLDADkye---------------------ndpELEKIRKXRNYsWXDIITICkdtlpnYEEKIKXFfE 98  house mouse
4QGN_A        32 LEQLRRlgVLYWKLDADkye---------------------ndpELEKIRRERNYsWMDIITICkdklpnYEEKIKMFyE 90  human
KIG17697      17 VEHLRSvgVTYECLNLVdna---------------------yqpSLDALKRERGYiEQDVIELHpdt-anLGAICAKFaD 74  Enhygromyxa salina
OGR52579      14 ADKLMEegIYYQKIPTEnf-----------------------qpSLDKIKKSWRYsAQDIVELYpam-pnLDAICAKFdK 69  Elusimicrobia b...
WP_053235162  14 GDTLRAegVHHDHHDVGah-----------------------daPLAALMREHRYvERDQVELRptt-pdLDALLAKFdR 69  Sandaracinus am...
WP_076312791  20 RNYLKDcnVLYEQWDSTklpaelkghklls-deeqqqvldlykeEISELAGRHGYqQWEVISLSnat-anLEGELAELdE 97  Paenibacillus s...
AMV35173      20 SAFLKPfgIWYEHWQVAgrlksdasd---------tdilteyqpEIDAVKAKGGYvTADVINVHpqt-pnLDAMLQKFnK 89  Pirellula sp. S...
OYW75502      20 RSFLLPhgIHYDRWPVAgridrdasq---------eeilaayapEVEALKQSGGYiTADVVNVTpev-pnLQEMLNKFnQ 89  Verrucomicrobia...
WP_081735403  32 HSFLEEqgVYYERWEGNhtpssyeerllls-deektellriyepSIADFAERKGYkQWDIVILSe----qTPDMLQKFgS 106 Paenibacillus s...
WP_094043907  20 ADFCRRqdIYYERWDADrlagwerdpgpklseseqariisayeeEIKRLAERRAYlNWDAVLMSd----aAPELLAKFgT 95  Cohnella
Feature 1         # #   #                                       #                        
1VR3_A        99 EHLHlDEEIRYILeGSGYFDVRdke-dkWIRISXekGDXITLPaGIYHRFTLDEkNYVKAXRLFVGEPVWTP 169 house mouse
4QGN_A        91 EHLHlDDEIRYILdGSGYFDVRdke-dqWIRIFMekGDMVTLPaGIYHRFTVDEkNYTKAMRLFVGEPVWTA 161 human
KIG17697      75 EHLHtDDEVRFVLaGEGIFDIRdda-drWMRVTVeaGDLIVVPkDRYHRFYLTDsQQIRCVRLFQDSAGWVP 145 Enhygromyxa salina
OGR52579      70 EHIHtDDEVRFVLnGEGIFDIRskk-dqWMRLLVekGDFILVPaNRNHLFYLTPtKTIQCVRLFKENPSWTP 140 Elusimicrobia bacterium...
WP_053235162  70 EHTHdEDEVRYVLeGEAIFDIRsrd-drWMRVVVseGDLIVVPkQRHHRFELGAtRTVRAVRLFQDRKGWVP 140 Sandaracinus amylolyticus
WP_076312791  98 MHAHgDEGAHAILsGNGILSMKddn-sgYFDIELtaGDVIVIPeNTPHSFALMKnKEVIAVRLTIKENGWTL 168 Paenibacillus sp. FSL H...
AMV35173      90 EHTHsEDEVRFTVsGKGVFHLHpeg-gpVFGVTVesGDMINVPrGMKHWFNLCDdHHIRCIRFFEDATGWAP 160 Pirellula sp. SH-Sr6A
OYW75502      90 EHRHaEDEVRFIVkGRGIFYINpesgdpVFSIEIeeGDLINVPaGTKHWFDLCAdRTIRAIRLFREKAGWTP 161 Verrucomicrobia bacteri...
WP_081735403 107 LHYHlDDEVRIVLgGAGVFTIKdeggsgYFELTAlpGDVVVVPaHRPHAFRLSErPQFIVVRLFEGEVSSVT 178 Paenibacillus sp. G1
WP_094043907  96 LHTHhEEEVRVLAdGGFAFVIRnvngdgYFELHVeeGDVFALPaNRPHAFKLGPqRKAVVVRLFSGEIGSVM 167 Cohnella

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