1YWL


Conserved Protein Domain Family
GIY-YIG_SLX1_like

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cd10449: GIY-YIG_SLX1_like 
Click on image for an interactive view with Cn3D
Catalytic GIY-YIG domain of yeast structure-specific endonuclease subunit SLX1 and its homologs
Structure-specific endonuclease subunit SLX1 is a highly conserved protein from yeast to human, with an N-terminal GIY-YIG endonuclease domain and a C-terminal PHD-type zinc finger postulated to mediate protein-protein or protein-DNA interaction. SLX1 forms active heterodimeric complexes with its SLX4 partner, which has additional roles in the DNA damage response that are distinct from the function of the heterodimeric SLX1-SLX4 nuclease. In yeast, the SLX1-SLX4 complex functions as a 5' flap endonuclease that maintains ribosomal DNA copy number, where SLX1 and SLX4 are shown to be catalytic and regulatory subunits, respectively. This endonuclease introduces single-strand cuts in duplex DNA on the 3' side of junctions with single-strand DNA. In addition to 5' flap endonuclease activity, human SLX1-SLX4 complex has been identified as a Holliday junction resolvase that promotes symmetrical cleavage of static and migrating Holliday junctions. SLX1 also associates with MUS81, EME1, C20orf94, PLK1, and ERCC1. Some eukaryotic SLX1 homologs lack the zinc finger domain, but possess intrinsically unstructured extensions of unknown function. These unstructured segments might be involved in interactions with other proteins.
Statistics
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PSSM-Id: 198396
Aligned: 38 rows
Threshold Bit Score: 71.4692
Created: 7-Oct-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative activeputative metalGIY-YIG
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on similarity to the sequence of Enterobacteria phage T4 homing endonuclease I-Tevi (1LN0).
  • Comment:The last conserved motif (invariant Asn residue) of GIY-YIG homing endonuclease I-TevI (1LN0) is completely absent in some of the SLX-1 family members.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #           # #       #   #                                  #       
1YWL_A        8 YFYVLLCq--DGSFYGGYTtEPERRLTEHNSGtgakytrlakrrPVIMIHTEKFEtrsEATKAEAAFKKLT 76  Enterococcus faecalis
ADV49515      4 YVYVLESe-kDQRWYKGHTvDIDKRIKEHNSGktkst---kgfiPWRLVCFEVFEtreEAILREKYFKTGS 70  Cellulophaga algicola DSM...
CBX31218      3 YTYVLYSk-kDGNFYTGFTqDLKLRFEQHTKGqvest---kdrrPLELVYYEACLnrsDATHREKYLKTYH 69  uncultured Desulfobacteri...
YP_001960337  3 YVYVLRSk-kDEHYYIGYTsDLDRRIDEHNQGfskst---ahrrPLELVYYEACRsskDAMHREKYLKSTY 69  Chlorobium phaeobacteroid...
ZP_01062524   3 FVYILESl-sSGRKYVGFTqNLDARLLEHNRGnvtst---kpfrPWKRIYSEEQPtqiEARMREKYLKSAA 69  Leeuwenhoekiella blandens...
YP_676842     3 FIYIIFSa-kVCRKYIGQTeDLALRLEQHNSNfyksy--tnnkgPWVLIHFECYEtrsEALRREKYLKSGA 70  Cytophaga hutchinsonii AT...
YP_004316714  5 YVYAIRSe-rDGRIYVGLSqNVEKRLNEHNEGrtkst---kgyiPWKLIYKEEVGnrlTARKREKYYKSGI 71  Sphingobacterium sp. 21
ZP_03127248   6 YVYILVSeadPTRYYVGRTdDLEDRLRRHNAGevsyt---sdhrPWKINVAIAFHdrtKAIDFEKYLKTHS 73  Chthoniobacter flavus Ell...
EDY83662     12 YVYVLVSg-dGKHRYVGTTqNLEQRLKKHNSGgvpht---srhvPWTIETATAFRaksKALAFEKYLKSGS 78  Verrucomicrobiae bacteriu...
YP_004043408  3 TVYAIRSl-tRNYIYVGMTsDLDERLQRHNAGyeqtt---kpyrPFELIYKEEVEsriEARNREKYFKSGV 69  Paludibacter propionicige...

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